Elemental content in the nucleus and in yolk and yolk‐free cytoplasm of developing <i>Rana pipiens</i> oocytes: An X‐ray microanalysis study

Labadie, D. R. L.; Pool, Thomas B.; Smith, Nancy R.; Cameron, I. L.

doi:10.1002/jcp.1041090111

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…X-ray microanalysis has previously been used for determining chemical composition of oocytes. Increases in Na, Mg, C1, and K concentrations in the nuclei and cytoplasm of growing ovarian frog oocytes were reported [Runa pipiens, LaBadie et al, 1981 andXenopus luevis, LaBadie et al, 19831. Nugent et a1 [1981] used X-ray microanalysis to verify the distribution sites of calcium in the mitochondria, membranes, and cortical vesicles of the zebra fish (Brunchydunio).…”

Section: Discussionmentioning

confidence: 99%

Changes in elemental composition of fertilized and unfertilized northern pike (Esox lucius) eggs incubated in buffer with and without dimethyl sulfoxide: An X‐ray microanalysis study

Schmehl¹,

Graham²

1986

Gamete Research

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Fertilized and unfertilized eggs from the northern pike (Esox lucius) were incubated 2 hr in buffer with 0 and 10% (v/v) dimethyl sulfoxide and then quickly frozen in the wells of aluminum blocks submerged in liquid nitrogen. Control eggs and ovarian fluid were similarly frozen immediately after collection. The frozen eggs were sectioned, freeze dried, mounted on stubs, and carbon coated. X‐ray microanalysis was used to determine changes in element levels and dimethyl sulfoxide (Me2SO) penetration in the zona radiata, cytoplasm, cortical alveoli, and egg yolk. Unfertilized eggs incubated without Me2SO showed decreased levels of Na, Cl, and K in the zona radiata; fertilized eggs, incubated without Me2SO showed decreased levels of Na, P, and Cl in the zona radiata and increased levels of K in the cytoplasm; unfertilized eggs, incubated with 10% Me2SO showed decreased Na and Cl in the zona radiata, decreased K in the cytoplasm and increased K in the cortical alveoli; fertilized eggs incubated in buffer with 10% Me2SO showed decreased levels of Na, P, Cl, and K (zona radiata), P, Cl, and K (cytoplasm), Na (yolk), and increased Cl in the yolk (all P<.01). Me2SO (v/v) levels reached 1.5‐3.1% in the zona radiata, 0‐3.2% in cytoplasm, 2.3‐8.7% in cortical alveoli, and 0‐1.6% in the yolk. Unfertilized eggs showed more Me2SO penetration than fertilized eggs.

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Section: Discussionmentioning

confidence: 99%

Changes in elemental composition of fertilized and unfertilized northern pike (Esox lucius) eggs incubated in buffer with and without dimethyl sulfoxide: An X‐ray microanalysis study

Schmehl¹,

Graham²

1986

Gamete Research

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Changes in water proton relaxation times and in nuclear to cytoplasmic element gradients during meiotic maturation of Xenopus oocytes

Cameron

Labadie

Hunter

et al. 1983

Journal Cellular Physiology

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Fully grown oocytes 1.2 mm in diameter were removed from Xenopus laevis ovaries and were exposed to progesterone (2.5 micrograms/ml in Ringer's solution) to induce completion of the first maturation division or germinal vesicle breakdown (GVBD). This process required 5.5 +/- 0.5 hr. Neither oocyte volume nor water content was observed to change throughout maturation. At selected times, the oocytes were quick frozen in liquid propane and cryosectioned. The sections were freeze-dried, and analyzed for K, Na, Cl, P, S, and Mg in millimolar per kilogram dry weight content in the nucleus and the yolk-free cytoplasm using electron probe X-ray microanalysis. Unstimulated oocytes showed significant nuclear to yolk-free cytoplasmic content gradients (N/C ratio) for the following elements: K (1.84), P (0.65), and S (1.56), but significant N/C content gradients were not found for Na and Mg. By 10 min after progesterone stimulation, a significant change in the N/C ratio of the following elements had occurred due to a rapid increase in nuclear content: K (2.29), Cl (2.11). A significant N/C ratio for Mg (1.35) had developed by 10 min after progesterone stimulation and a significant N/C ratio for Na (2.07) had developed by 45 min. In addition the following elements showed significant content increases in both the nucleus and the yolk-free cytoplasm from the time prior to progesterone stimulation to the time just prior to GVBD at 240 min: K, Na, Cl, P, S, and Mg. Nuclear magnetic resonance measurements of the spin-lattice relaxation time (T1) of water proton in oocytes showed a significant increase in the T1 time after progesterone exposure. The changes in N/C ratios of specific elements and in the physical parameter of water proton relaxation time suggest that progesterone is responsible for inducing changes in the physicochemical interactions between various macromolecules, specific elements, and water.

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Water, potassium, and sodium during amphibian oocyte development

Tluczek¹,

Lau²,

Horowitz³

1984

Developmental Biology

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Elemental content in the nucleus and in yolk and yolk‐free cytoplasm of developing Rana pipiens oocytes: An X‐ray microanalysis study

Cited by 3 publications

References 13 publications

Changes in elemental composition of fertilized and unfertilized northern pike (Esox lucius) eggs incubated in buffer with and without dimethyl sulfoxide: An X‐ray microanalysis study

Changes in elemental composition of fertilized and unfertilized northern pike (Esox lucius) eggs incubated in buffer with and without dimethyl sulfoxide: An X‐ray microanalysis study

Changes in water proton relaxation times and in nuclear to cytoplasmic element gradients during meiotic maturation of Xenopus oocytes

Water, potassium, and sodium during amphibian oocyte development

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