Elemental composition of globoid crystals in protein bodies of wheat grain grown on soil treated with sewage sludge

Spitzer, Ernest; Webber, M. P. C.; Lott, John N. A.

doi:10.1139/b81-055

Cited by 6 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2) with more than a 6-fold increase in grain Zn concentration (Table 2). Spitzer et al (1981) found a 2-fold increase in Zn concentration in wheat grain (up to 78 mg kg -t) when grown on sludge-amended soil. Similarly, Welch (1986) reported that increased fertilisation of other grain crops resulted in increased Zn concentration in mature seed.…”

Section: Discussionmentioning

confidence: 93%

Importance of seed Zn content for wheat growth on Zn-deficient soil

1995

View full text Add to dashboard Cite

Two wheat (Triticum aestivum L.) genotypes (Zn-efficient Excalibur and Zn-inefficient Gatcher) were grown from low-Zn (around 250 ng Zn/seed) and high-Zn seed (around 700 ng Zn/seed) in a Zn-deficient siliceous sand fertilised with 0, 0.05, 0.2, 0.8 or 3.2 mg Zn kg -1 soil. At maturity, plants derived from the high-Zn seed had bigger grains and produced more grains than plants grown from the low-Zn seed when fertilised with nil Zn. Plants grown from high-Zn seed produced more grain dry matter per unit of Zn absorbed by the above-ground parts, transported a larger proportion of absorbed Zn to the grain, and approached the maximum harvest index with the fertilisation rate of 0.05 mg Zn kg-l compared to 0.2 mg Zn kg-1 soil required for plants derived from the low-Zn seed. The Zn-efficient cv. Excalibuir had greater fertiliser efficiency when fertilised with 0.05 mg Zn kg-1 soil and a greater harvest index at nil Zn treatment compared to the Zn-inefficient cv. Gatcher. Zn-deficient plants produced seed with higher concentrations of all inorganic nutrients determined except Zn. There was a positive relationship between grain dry weight and Zn concentrations in youngest expanded blades sampled at 6 weeks of growth. It is concluded that crops grown from seed containing higher Zn content have a distinct advantage which culminates in greater yield when grown in soils of low Zn status.

show abstract

Section: Discussionmentioning

confidence: 93%

Importance of seed Zn content for wheat growth on Zn-deficient soil

1995

View full text Add to dashboard Cite

show abstract

“…In this study, GCs rich in Zn were found in maize radicle and coleorhiza. In dry seeds the presence of Zn in GCswas not yet found, even though investigated wheat seeds were from plants grown on soil contaminated by Zn (Spitzer et al, 1981). Existence of the capacity to bind a substantial amount of Zn in dense globular deposits, identified as Zn-phytin salt, was described in roots of Lemna and Deschampsia plants challenged with high levels of Zn (Van Steveninck et al, 1987.…”

Section: Results Of Our Edx Analyses Of Scutellarmentioning

confidence: 99%

Structure of Protein Bodies and Elemental Composition of Phytin from Dry Germ of Maize (Zea mays L.)

1992

View full text Add to dashboard Cite

Protein bodies (PBs) from the epidermis and cortex of the radicle, as well as from coleorhizal and scutellar parenchyma of dry maize seeds were studied with the emphasis on presence and elemental composition of globoid crystals. PBs with variable structure were found in investigated tissues. Radicular epidermis was the only investigated tissue containing structurally uniform populations of PBs. They were filled by a homogeneous matrix without globoid crystals or their remnants. PBs structure and amount of proteinaceous matrix varied in the same cell of coleorhiza and scutellum. In the case of radicular cortex, the structure of PBs differed between analyzed individuals. Globoid crystals of various sizes were found in PBs from scutellum, coleorhiza, and from inner layers of radicular cortex. The largest globoid crystals (up to 2.0 μ in diameter) were seen in the scutellum. Energy‐dispersive X‐ray microanalysis revealed the presence of P,K. Mg, and some traces of Fe and Zn in the majority of globoid crystals from the scutellum. Globoid crystals from coleorhiza and radicular cortex were rich in Zn and Ca. The presence of considerable Zn was usually accompanied by high Ca, at the expense of K and Mg. As EDX analyses revealed significant differences in Zn and Zn/P values between analyzed tissues, it is probable that the majority of Zn is preferably accumulated in globoid crystals of some tissues of maize germ.

show abstract

“…The facts that phytic acid to Zn molar ratio decreases with zinc fertilization, while protein content increases, supports the hypothesis that some proteins are more important in Zn binding than phytic acid [ 28 , 29 ]. Furthermore, in the grain, phytic acid localizes in the protein storage vacuoles of the aleurone cells, and the globoids do not contain zinc, as measured by ICP-MS [ 30 , 31 , 32 ]. On the other hand, increased phytochelatin synthesis correlates with zinc accumulation in vegetative vacuoles only [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Enrichment and Identification of the Most Abundant Zinc Binding Proteins in Developing Barley Grains by Zinc-IMAC Capture and Nano LC-MS/MS

2018

View full text Add to dashboard Cite

Background: Zinc accumulates in the embryo, aleurone, and subaleurone layers at different amounts in cereal grains. Our hypothesis is that zinc could be stored bound, not only to low MW metabolites/proteins, but also to high MW proteins as well. Methods: In order to identify the most abundant zinc binding proteins in different grain tissues, we microdissected barley grains into (1) seed coats; (2) aleurone/subaleurone; (3) embryo; and (4) endosperm. Initial screening for putative zinc binding proteins from the different tissue types was performed by fractionating proteins according to solubility (Osborne fractionation), and resolving those via Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) followed by polyvinylidene fluoride (PVDF) membrane blotting and dithizone staining. Selected protein fractions were subjected to Zn2+-immobilized metal ion affinity chromatography, and the captured proteins were identified using nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS). Results: In the endosperm, the most abundant zinc binding proteins were the storage protein B-hordeins, gamma-, and D-hordeins, while in the embryo, 7S globulins storage proteins exhibited zinc binding. In the aleurone/subaleurone, zinc affinity captured proteins were late abundant embryogenesis proteins, dehydrins, many isoforms of non-specific lipid transfer proteins, and alpha amylase trypsin inhibitor. Conclusions: We have shown evidence that abundant barley grain proteins have been captured by Zn-IMAC, and their zinc binding properties in relationship to the possibility of zinc storage is discussed.

show abstract

Elemental composition of globoid crystals in protein bodies of wheat grain grown on soil treated with sewage sludge

Cited by 6 publications

References 29 publications

Importance of seed Zn content for wheat growth on Zn-deficient soil

Importance of seed Zn content for wheat growth on Zn-deficient soil

Structure of Protein Bodies and Elemental Composition of Phytin from Dry Germ of Maize (Zea mays L.)

Enrichment and Identification of the Most Abundant Zinc Binding Proteins in Developing Barley Grains by Zinc-IMAC Capture and Nano LC-MS/MS

Contact Info

Product

Resources

About