Elemental bio-imaging using laser ablation-triple quadrupole-ICP-MS

Bishop, David; Clases, David; Fryer, Fred; Williams, Elizabeth D.; Wilkins, Simon; Hare, Dominic J.; Cole, Nerida; Kärst, Uwe; Doble, Philip

doi:10.1039/c5ja00293a

Cited by 66 publications

(54 citation statements)

References 44 publications

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“…The NWR213 was fitted with a standard two-volume cell with a 10 cm x 10 cm scanning area. Standard operating parameters for this system were used as previously reported (Bishop et al, 2015). Mass-to-charge (m/z) ratios for carbon (13), phosphorus (31) and iron (56) were acquired.…”

Section: La-icp-ms Analysismentioning

confidence: 99%

Laser ablation-inductively coupled plasma-mass spectrometry imaging of white and gray matter iron distribution in Alzheimer's disease frontal cortex

et al. 2016

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Iron deposition in the brain is a feature of normal aging, though in several neurodegenerative disorders, including Alzheimer's disease, the rate of iron accumulation is more advanced than in age-matched controls. Using laser ablation-inductively coupled plasma-mass spectrometry imaging we present here a pilot study that quantitatively assessed the iron content of white and gray matter in paraffin-embedded sections from the frontal cortex of Alzheimer's and control subjects. Using the phosphorus image as a confirmed proxy for the white/gray matter boundary, we found that increased intrusion of iron into gray matter occurs in the Alzheimer's brain compared to controls, which may be indicative of either a loss of iron homeostasis in this vulnerable brain region, or provide evidence of increased inflammatory processes as a response to chronic neurodegeneration. We also observed a trend of increasing iron within the white matter of the frontal cortex, potentially indicative of disrupted iron metabolism preceding loss of myelin integrity. Considering the known potential toxicity of excessive iron in the brain, our results provide supporting evidence for the continuous development of novel magnetic resonance imaging approaches for assessing white and gray matter iron accumulation in Alzheimer's disease.

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Section: La-icp-ms Analysismentioning

confidence: 99%

Laser ablation-inductively coupled plasma-mass spectrometry imaging of white and gray matter iron distribution in Alzheimer's disease frontal cortex

et al. 2016

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“…Although the examples reported above demonstrates the possibility to apply LA‐ICP‐MS for bio‐imaging metals concentrations of various brain areas, the limits of this approach must be taken into account. Besides the matrix effects that can be partially overcome with multipoint calibrations constructed from matrix‐matched standards or the double isotope dilution strategy, we highlight that mapping metal ions concentrations of the brain still remains a challenging task also because of sensitivity issues; only results obtained with the same experimental techniques from different areas should be compared. Indeed, systematic differences in data obtained with different experimental approaches are very much expected and must be considered …”

Section: Beyond the Aβ Peptides: The Contribution Of Mass Spectrometrmentioning

confidence: 99%

Mass spectrometry is a multifaceted weapon to be used in the battle against Alzheimer's disease: Amyloid beta peptides and beyond

Grasso

2018

Mass Spectrometry Reviews

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Amyloid-β peptide (Aβ) accumulation and aggregation have been considered for many years the main cause of Alzheimer's disease (AD), and therefore have been the principal target of investigation as well as of the proposed therapeutic approaches (Grasso [2011] Mass Spectrom Rev. 30: 347-365). However, the amyloid cascade hypothesis, which considers Aβ accumulation the only causative agent of the disease, has proven to be incomplete if not wrong. In recent years, actors such as metal ions, oxidative stress, and other cofactors have been proposed as possible co-agents or, in some cases, main causative factors of AD. In this scenario, MS investigation has proven to be fundamental to design possible diagnostic strategies of this elusive disease, as well as to understand the biomolecular mechanisms involved, in the attempt to find a possible therapeutic solution. We review the current applications of MS in the search for possible Aβ biomarkers of AD to help the diagnosis of the disease. Recent examples of the important contributions that MS has given to prove or build theories on the molecular pathways involved with such terrible disease are also reviewed.

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“…Improvements in the limits of detection of LA-ICPMS may yield instruments capable of quantifying endogenous metals at subcellular resolution. 58,64,66,67 …”

Section: Technologies For Imaging Total Metal Poolsmentioning

confidence: 99%

Analytical Methods for Imaging Metals in Biology: From Transition Metal Metabolism to Transition Metal Signaling

2016

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Elemental bio-imaging using laser ablation-triple quadrupole-ICP-MS

Abstract: Elemental bio-imaging using the technological advances of ICP-QQQ-MS.

Cited by 66 publications

References 44 publications

Laser ablation-inductively coupled plasma-mass spectrometry imaging of white and gray matter iron distribution in Alzheimer's disease frontal cortex

Laser ablation-inductively coupled plasma-mass spectrometry imaging of white and gray matter iron distribution in Alzheimer's disease frontal cortex

Mass spectrometry is a multifaceted weapon to be used in the battle against Alzheimer's disease: Amyloid beta peptides and beyond

Analytical Methods for Imaging Metals in Biology: From Transition Metal Metabolism to Transition Metal Signaling

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