Elemental analysis of soil extracts and plant tissue ash by plasma emission spectroscopy

Jones, J. Benton

doi:10.1080/00103627709366727

Cited by 195 publications

(91 citation statements)

References 6 publications

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“…UV-visible spectroscopic studies indicate that CprK does not contain a chromophore with an absorption spectrum in the 300 -640-nm region. Based on 30-element inductively coupled plasma-emission spectrometry (Chemical Analysis Laboratory, University of Georgia, Athens, GA) (26), which includes all metals known to be present in enzymes, the only metal found was zinc, and this was present in only substoichiometric amounts (0.2-0.3 per mol). However, as described below, zinc does not appear to be required for activity.…”

Section: Resultsmentioning

confidence: 99%

Regulation of Anaerobic Dehalorespiration by the Transcriptional Activator CprK

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Kolarik

Ragsdale

2004

Journal of Biological Chemistry

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Desulfomonile, Desulfitobacterium, and Dehalobacter are anaerobic microbes that can derive energy from the reductive dehalogenation of chlorinated organic compounds, many of which are environmental pollutants. There is very little information about how anaerobic dehalorespiration is regulated. An open reading frame within the Desulfitobacterium dehalogenans chlorophenol reductase (cpr) gene cluster (cprK) was proposed to be a transcriptional regulatory protein (Smidt, H., van Leest, M., van der Oost, J., and deVos, W. M. (2000) J. Bacteriol. 182, 5683-5691). We have cloned, actively overexpressed in Escherichia coli, and purified to homogeneity the D. dehalogenans CprK. The results of electrophoretic mobility shift assays, DNA footprinting studies, and promoter-lac fusion experiments indicate that CprK is a transcriptional activator of the cpr gene cluster. CprK binds 3-chloro-4-hydroxyphenylacetate (CHPA) with high affinity (K d ‫؍‬ 3.5 M, determined by isothermal titration calorimetry), which promotes its specific interaction with a DNA sequence (TTAAT-N4-ACTAA) located upstream of the ؊35 and ؊10 promoter regions of several cpr genes and activates transcription of these genes. Binding to the upstream "box" sequence increases the affinity of CprK for CHPA by ϳ10-fold (K d ‫؍‬ 0.4 M, determined by electrophoretic mobility shift assays). Chlorophenylacetate, which lacks the ortho-hydroxy group, and hydroxyphenylacetate, lacking the chlorine group, do not activate transcription or promote DNA binding, even at millimolar concentrations, at least 1000-fold higher than the K d value for CHPA. Lacking metals, CprK is oxygen-sensitive. Oxidation by diamide, which converts thiols to the disulfide, inactivates CprK, and reduction of the oxidized protein by dithiothreitol fully restores DNA binding, indicating that CprK is redox-regulated and is active only when reduced. This is the first reported characterization of a transcriptional regulator of anaerobic dehalorespiration.

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Section: Resultsmentioning

confidence: 99%

Regulation of Anaerobic Dehalorespiration by the Transcriptional Activator CprK

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Kolarik

Ragsdale

2004

Journal of Biological Chemistry

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“…The amount of protein-bound cobamide was determined spectrophotometrically after conversion of the cobamide to the dicyano form (19) as previously described (24). The metal content was analyzed by plasma emission spectroscopy (16) at the Chemical Analysis Laboratory, University of Georgia. Chemicals.…”

Section: Methodsmentioning

confidence: 99%

Electron paramagnetic resonance spectroscopic and electrochemical characterization of the partially purified N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanosarcina mazei Gö1

Becher

Gottschalk

et al. 1995

J Bacteriol

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Methane is a major terminal product of the anaerobic degradation of biomass. It is produced by methanogenic archaea from substrates such as acetate, H 2 ϩ CO 2 , methanol, methylamines, and formate. Two reactions involved in methanogenesis from H 2 ϩ CO 2 are coupled to energy-conserving ion translocations: the H 2 -heterodisulfide reductase functions as a primary proton pump (6) and the corrinoid enzyme N 5

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“…Plant P2O5 uptake (g plant - 효인산분석법이다 (Yoon, 1988 (Munter, 1990;Jones, 1997;Mallarino, 2003 Table 1 과 같다. (Hylander et al, 1995;Eckert and Watson, 1996;Nathan et al, 2002).…”

Section: Soil Testmentioning

confidence: 99%