Elemental 2-D mapping and changes in leaf iron and chlorophyll in response to iron re-supply in iron-deficient GF 677 peach-almond hybrid

Jiménez, Sergio; Morales, Fermı́n; Abadı́a, Anunciación; Abadı́a, Javier; Sánchez, María Ángeles Moreno; Gogorcena, Yolanda

doi:10.1007/s11104-008-9735-9

Cited by 40 publications

(25 citation statements)

References 53 publications

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“…Reduction in plant growth of deficient plants would produce apparently higher metabolite concentrations. However, in this study, there were not significant changes in root growth between treatments, as previously reported (Jiménez et al, 2009). Therefore, sucrose accumulation (and any other metabolite accumulation) seems to be independent of a possible concentration effect in deficient plants.…”

Section: Iron Deficiency Induces the Root Accumulation Of Sugars Orgsupporting

confidence: 71%

“…On the other hand, the role suggested for the organic acids comprise its use as chelates of iron in soil or xylem, a source to obtain reductive power and a source of anaplerotic carbon in leaves , although the excretion of organic acids in Prunus rootstocks remain unknown. Indeed, since iron deficiency can induce the accumulation of other metals such as Mn, Zn and Cu (Jiménez et al, 2009), organic and amino acids could be involved in metal binding (Sharma and Dietz, 2006) to avoid oxidative damage due to other catalytic ions.…”

Section: Iron Deficiency Stimulates the Root Pepc Activity In The Tolmentioning

confidence: 99%

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Metabolic response in roots of Prunus rootstocks submitted to iron chlorosis

Jiménez

Ollat

Deborde

et al. 2011

Journal of Plant Physiology

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Iron deficiency induces several responses to iron shortage in plants. Metabolic changes occur to sustain the increased iron uptake capacity of Fe-deficient plants. We evaluated the metabolic changes of three Prunus rootstocks submitted to iron chlorosis and their different responses for tolerance using measurements of metabolites and enzymatic activities. The more tolerant rootstocks Adesoto (Prunus insititia) and GF 677 (Prunus amygdalus×Prunus persica), and the more sensitive Barrier (P. persica×Prunus davidiana) were grown hydroponically in iron-sufficient and -deficient conditions over two weeks. Sugar, organic and amino acid concentrations of root tips were determined after two weeks of iron shortage by proton nuclear magnetic resonance spectroscopy of extracts. Complementary analyses of organic acids were performed by liquid chromatography coupled to mass spectrometry. The major soluble sugars found were glucose and sucrose. The major organic acids were malic and citric acids, and the major amino acid was asparagine. Iron deficiency increased root sucrose, total organic and amino acid concentrations and phosphoenolpyruvate carboxylase activity. After two weeks of iron deficiency, the malic, citric and succinic acid concentrations increased in the three rootstocks, although no significant differences were found among genotypes with different tolerance to iron chlorosis. The tolerant rootstock Adesoto showed higher total organic and amino acid concentrations. In contrast, the susceptible rootstock Barrier showed lower total amino acid concentration and phosphoenolpyruvate carboxylase activity values. These results suggest that the induction of this enzyme activity under iron deficiency, as previously shown in herbaceous plants, indicates the tolerance level of rootstocks to iron chlorosis. The analysis of other metabolic parameters, such as organic and amino acid concentrations, provides complementary information for selection of genotypes tolerant to iron chlorosis.

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Section: Iron Deficiency Induces the Root Accumulation Of Sugars Orgsupporting

confidence: 71%

Section: Iron Deficiency Stimulates the Root Pepc Activity In The Tolmentioning

confidence: 99%

Metabolic response in roots of Prunus rootstocks submitted to iron chlorosis

Jiménez

Ollat

Deborde

et al. 2011

Journal of Plant Physiology

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“…Iron was localized in the main veins; a similar behaviour was observed for K, Zn and Br. Iron localization in the main veins with low concentrations in the interveinal mesophyll areas was also observed by Jimenéz et al (2009) in leaves of Fe-sufficient and Fe-deficient Prunus, by using synchrotron µ-XRF. However, compared to the data presented in the present paper, they used a lower resolution (100 vs 20 µm) with a consequent limitation on the level of detail achieved in elemental distribution maps.…”

Section: Resultsmentioning

confidence: 67%

Micro-analytical, physiological and molecular aspects of Fe acquisition in leaves of Fe-deficient tomato plants re-supplied with natural Fe-complexes in nutrient solution

et al. 2009

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It is well known that in the rhizosphere soluble Fe sources available for plants are mainly a mixture of complexes between the micronutrient and organic ligands such as organic acids and phytosiderophores (PS) released by roots, microbial siderophores as well as fractions of humified organic matter. In the present work, mechanisms of Fe acquisition operating at the leaf level of plants fed with different Fe-complexes were investigated at the micro-analytical, physiological and molecular levels. Fe-deficient tomato plants (Solanum Lycopersicum L., cv. 'Marmande') were fed for 24 h with a solution (pH 7.5) containing 1 A mu M Fe as Fe-PS, Fe-citrate or Fe-WEHS. Thereafter, leaf tissue was used for the visualization of Fe distribution, measurements of Fe content, reduction and uptake, and evaluation of expression of Fe-chelate reductase (LeFRO1), Fe-transporter (LeIRT1) and Ferritin (Ferritin2) genes. Leaf discs isolated from Fe-deficient plants treated for 24 h with Fe-WEHS developed higher rates of translocation, Fe-chelate reduction and Fe-59 uptake as compared to plants supplied with Fe-citrate or Fe-PS. Leaves of plants treated with Fe-WEHS also showed higher transcript levels of LeFRO1, LeIRT1 and Ferritin2 genes with respect to plants fed with the other Fe-sources. Data obtained support the idea that the efficient use of Fe complexed to WEHS-like humic fractions involves, at least in part, also the activation of Fe-acquisition mechanisms operating at the leaf level

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“…In our study, only Mn This effect seems a consequence of the diminished dilution of the normal Fe concentration in leaves (Römheld, 2000). An alternative explanation for the "chlorosis paradox" is the existence of Fe pools which have precipitated in the leaf (Jiménez et al, 2009 (Marschner, 1995 -chelate reductase (FC-R) at the plasma membrane of the rhizodermal cells (Römheld and Marschner, 1986;García-Mina et al, 2013). Proton extrusion and the release of organic compounds could help in the solubilization of Fe compounds.…”

Section: Discussionmentioning

confidence: 40%

Xylem sap mineral analyses as a rapid method for estimation plant-availability of Fe, Zn and Mn in carbonate soils: a case study in cucumber

Bityutskii

Yakkonen

Petrova

et al. 2017

J. Soil Sci. Plant Nutr.

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Low plant-availability of iron (Fe), zinc (Zn) and manganese (Mn) leads to micronutrient deficiency, causing significant yield reductions of crops throughout the world, especially in calcareous soils. This study was performed in order to evaluate the efficiency of xylem sap analysis in the determination of Fe, Zn and Mn availability in plants (Cucumis sativus L.) affected by calcium carbonate (CaCO 3 ) levels. A soil with six levels of CaCO 3 (0−10% DW) was used. We performed a combination approach, including analysis of the soil mobility of micronutrients using different extractants (water, DTPA-TEA and ammonium acetate), as well as xylem and shoot elemental analysis. Generally, application of CaCO 3 resulted in a pH increase of the bulk soil of 1.4−2.2 pH units; extractability of all micronutrients was significantly decreased 1.4−4.2 times, irrespective of the extracting solution. Xylem sap Fe, Zn and Mn concentrations were significantly correlated with the respective concentrations in the soil extracting solutions. By contrast, only shoot concentrations of Zn and Mn, but not of Fe, were linearly correlated with their extractable forms. With electrothermal atomic absorption spectrometry, changes in xylem sap concentrations of micronutrients were detected without preliminary mineralization of plant material, in contrast to shoot analysis. Our results demonstrate that xylem sap analysis offers the advantages of a simple characterization of multi-microelement availability in plants under CaCO 3 stress.

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Elemental 2-D mapping and changes in leaf iron and chlorophyll in response to iron re-supply in iron-deficient GF 677 peach-almond hybrid

Cited by 40 publications

References 53 publications

Metabolic response in roots of Prunus rootstocks submitted to iron chlorosis

Metabolic response in roots of Prunus rootstocks submitted to iron chlorosis

Micro-analytical, physiological and molecular aspects of Fe acquisition in leaves of Fe-deficient tomato plants re-supplied with natural Fe-complexes in nutrient solution

Xylem sap mineral analyses as a rapid method for estimation plant-availability of Fe, Zn and Mn in carbonate soils: a case study in cucumber

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