Elektronenmikroskopische Untersuchungen von intranucle�ren Einschlu�k�rpern

Wessel, W.

doi:10.1007/bf00955258

Cited by 54 publications

(6 citation statements)

References 12 publications

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“…The presence of whorls of membranes also suggests a transformation of cellular constituents in an unfavorable environment (3). Similar narrow openings and abnormal contents were described in livers of thioacetamide-treated rats (6) and colchicinetreated mice (11). If these inclusions formed in order to compensate for an altered nuclear surfacevolume ratio as we have previously proposed (8), their postulated role in increasing the effective area of the nuclear surface seems to have been lost.…”

Section: Enlarged Hepatic Cells--the Intranuclear In-supporting

confidence: 60%

“…This may be one of the reasons that all of these giant cells became moribund and eventually died (12). In this connection Wessel (11) makes an interesting point that the livers in which intranuclear inclusions occur are abnormal, but the tumor cells in which they occur, e.g. in his work, the Crocker ascites sarcoma, are healthy, growing cells.…”

Section: Enlarged Hepatic Cells--the Intranuclear In-mentioning

confidence: 99%

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An Electron Microscope Study of Intranuclear Inclusions in Mouse Liver and Hepatoma

Leduc

Wilson

1959

The Journal of Cell Biology

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An electron microscope study of intranuclear inclusions which occur in giant cells in a transplantable mouse hepatoma and in enlarged liver cells in mice fed a diet containing bentonite demonstrates that these inclusions are formed by invaginations of the nuclear envelope, and corroborates a previous histochemical study which revealed that the contents of the inclusions are of cytoplasmic origin.In the hepatoma cells the intranuclear inclusions are abundant, small, and situated close to the border of the nucleus, and there are wide openings from the cytoplasm into the invaginations whose contents include lipid droplets, ergastoplasm, and structurally normal mitochondria.In the enlarged liver cells the inclusions are fewer in number, generally much larger than those in the hepatoma, hence they extend deeper into the nucleus, and the interior is continuous with the cytoplasm through only a small opening. Some normal ergastoplasm is present within the inclusions but all other constituents are abnormal. Both normal and degenerating mitochondria occur in the cytoplasm but only degenerating ones are found within the inclusions.Both types of inclusions arise in greatly enlarged ceils in which an attempt is made to maintain the normal nuclear surface/nuclear volume ratio by the development of the invaginations of the nuclear envelope.

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Section: Enlarged Hepatic Cells--the Intranuclear In-supporting

confidence: 60%

Section: Enlarged Hepatic Cells--the Intranuclear In-mentioning

confidence: 99%

An Electron Microscope Study of Intranuclear Inclusions in Mouse Liver and Hepatoma

Leduc

Wilson

1959

The Journal of Cell Biology

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“…Though NI can be observed both in normal and neoplastic cells 6,32,33 , they are more frequently found in neoplastic cells 20 and are associated with clinical conditions such as diabetes, inflammatory, arteriosclerotic and neoplastic lesions, obesity and NAFLD 8,9,35 . It is documented that NI can be caused by damaging external influences, for example NI induction was observed in mouse livers after colchicine application and in kidney epithelia after chronic lead poisoning and X-ray irradiation 17 . Studies of De Oliveira et al in a NAFLD/NASH-HCC zebrafish model showed that glycogen accumulation/ballooning degeneration were associated with inflammation in the liver and with cancer progression 36 .…”

Section: Discussionmentioning

confidence: 99%

“…These early studies were based on mere description of morphological aspects of inclusions in light microscopy. Later, in vivo studies demonstrated the induction of NI by injections of colchicine or thioacetamide in mouse hepatocytes 16,17 . Ultrastructural characterization of NI in mouse liver and HCC revealed inclusions bounded by a double membrane originating from the nuclear envelope 18 .…”

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confidence: 99%

Characterization of two types of intranuclear hepatocellular inclusions in NAFLD

Schwertheim

Kälsch

Jastrow

et al. 2020

Sci Rep

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Nuclear inclusions (NI) are a common finding in hepatocytes from patients with liver disease especially in diabetes mellitus and non-alcoholic fatty liver disease (NAFLD) but studies examining the shape and content of these inclusions in detail are lacking. In this study we define two distinct types of NI in NAFLD: inclusions bounded by the nuclear membrane, containing degenerative cell organelles and heterolysosomes (type1) and inclusions with deposits of glycogen but without any kind of organelles and delimiting membrane (type2). NI in 77 paraffin-embedded patients of NAFLD including NAFL and non-alcoholic steatohepatitis (NASH) were analyzed. In 4–12% of type1 NI immunopositivity for the autophagy-associated proteins LC3B, ubiquitin, p62/sequestosome1, cathepsin D and cathepsin B were detected with co-localizations of ubiquitin and p62; type2 NI showed no immunoreactivity. Three-dimensional reconstructions of isolated nuclei revealed that NI type1 are completely enclosed within the nucleus, suggesting that NI, although probably derived from cytoplasmic invaginations, are not just simple invaginations. Our study demonstrates two morphologically different types of inclusions in NAFLD, whereby both gained significantly in number in advanced stages. We suggest that the presence of autophagy-associated proteins and degenerated organelles within type1 NI plays a role in disease progression.

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“…Examples of other situations where they have been described include liver and spleen cells in reticulum cell leukaemia of the mouse [1], epithelial cells from lichen planus lesions [7], liver cells following colchicine poisoning in the mouse [10], condylomata acuminata [4], cells of the Shope papilloma of rabbits [8] and the lesions of psoriasis [2], In line with the views of several of these authors we feel that the presence of intranuclear particles is an expression of changes in the cellular metabolism, and that a virus infec tion is merely one of the possible agents which may bring about such a change.…”

Section: Discussionmentioning

confidence: 99%

Intranuclear Particles in Keratoacanthoma: Possible Association with Malignant Degeneration

Staak¹,

Bergers²

1979

Dermatology

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Elektronenmikroskopische Untersuchungen von intranucle�ren Einschlu�k�rpern

Cited by 54 publications

References 12 publications

An Electron Microscope Study of Intranuclear Inclusions in Mouse Liver and Hepatoma

An Electron Microscope Study of Intranuclear Inclusions in Mouse Liver and Hepatoma

Characterization of two types of intranuclear hepatocellular inclusions in NAFLD

Intranuclear Particles in Keratoacanthoma: Possible Association with Malignant Degeneration

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