Elektronenmikroskopische Untersuchungen �ber den Einflu� der Hyaluronidase auf die Basalmembran der Glomerulumcapillaren mit besonderer Ber�cksichtigung der Permeabilit�tsfrage

Amon, H.; Gayer, J

doi:10.1007/bf01479427

Cited by 19 publications

(5 citation statements)

References 46 publications

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“…Marked protrusion from the GBM in the case of experimental nephri tis coincided with the disappearances of AMPS possibly related to the remarkable activity of (3-Gase in the epithelial cells with fusion of the foot processes overlying this portion. Some of this protrusion seems to be similar to the one reported by A mon and Gayer [ 18] in the rabbit administered bovine hyaluronidase. This suggests that although in experimental glomerulonephritis admin istered NTX consisted ofy-globulin fraction and may not have hyaluronidase activity, a similar enzymatic reaction might have aris en in the glomeruli.…”

Section: Discussionsupporting

confidence: 70%

“…Some of the investigators [12,20] regard it as sialic acid and others as nonsulfated AMPS, presumably HA [18]. A pre vious study by us [2] supported the possibil ity of the latter, since we have so far failed to stain this substance constantly by Carlo's [19] stain, which is the method of detecting sialic acid.…”

Section: Discussionmentioning

confidence: 83%

“…However, this might be a reason why many investigators after Michael et al [20] hesitate to use hyaluronic acid instead of the glomerular polyanion. Nevertheless, since Amon and Gayer [18] and recently Kawamura et al [21] reported the protru sion of GBM towards the epithelial side in rabbits administered with bovine hyaluron idase, it is almost sure that hyaluronic acid is present in the area inside or adjacent to the GBM, which influences the maintenance of the GBM structure. If we see the colloid iron-stained electron micrograph of the glo meruli, there is no area in the GBM itself where the colloid iron combines as strong as at the surface of the foot processes and endo thelial cells.…”

Section: Discussionmentioning

confidence: 99%

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Acid Mucopolysaccharide and One of Its Glomerular Degrading Enzymes β-Glucuronidase in Experimental and Human Glomerulonephritis

Matsuo¹,

Yamada²

1980

Kidney Blood Press Res

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In 1962, when the immune complex in nephritic glomerular basement membrane (GBM) was clarified as being a type of GBM thickening, Amon and Gayer reported a different type of thickening in the rabbit administered hyaluronidase (an enzyme to degrade hyaluronic acid) and named it ‘herniation’ of the GBM. As we have been interested for a long time in the disappearance of normally present nonsulfated AMPS, presumably hyaluronic acid (HA), from the glomeruli in humans and experimental animals with chronic glomerulonephritis, we wanted to observe the activity of the enzyme in these conditions. Since a suitable histochemical method for the precise evaluation of hyaluronidase is unavailable, we instead chose β-glucuronidase(β-Gase), which is also an enzyme which degrades HA. The principal study was performed by means of light- and electron-microscopic histochemistry of chronic glomerulonephritis produced experimentally in rats and compared the obtained results to those in human chronic glomerulonephritis. The high activity of β-Gase with a coincidental decrease of AMPS in the glomeruli was observed both in experimental and human chronic glomerulonephritis. The herniation type GBM thickening in the rat was coincidental with the enzyme localization with the disappearance of AMPS from foot processes of epithelial cells overlaying the lesion. The results might suggest the key role of β-Gase in the deformation of GBM in chronic glomerulonephritis in general.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

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Acid Mucopolysaccharide and One of Its Glomerular Degrading Enzymes β-Glucuronidase in Experimental and Human Glomerulonephritis

Matsuo¹,

Yamada²

1980

Kidney Blood Press Res

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“…The normal epithelial cytoplasm contains two groups of fine filamen tous elements: the larger are 50 to 85 A long and are more or less uniformly distributed in the cytoplasm while the smaller fibrils, about 30 A long, appear to be continuous with similar structures in the basement membrane. Striking changes of this membrane after intravenous administration of hyaluronidase have been reported by Amon & Gayer (12) three-layer arrangement. Presumably, a mucopolysaccharide stabilizes the configuration of the basement membrane.…”

Section: Glomerular Structure and Functionmentioning

confidence: 89%

Kidney, Water, and Electrolytes

Chinard¹

1964

Annu. Rev. Physiol.

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INTRODUCTIONThe evolution of renal physiology has followed an interesting course in the past few years and has reflected the different approaches made possible by the development of new tools and methods of investigation. The improve ment and extension of the micropipetting techniques have brought the study of the control of electrolyte and water excretion from a consideration of balances of input and outgo for the whole kidney to knowledge of the fluxes and their control in segments of individual nephrons. Studies of slices, of homogenates, and of structural components of cells are dovetailing with the micropipetting experiments to provide data relating metabolic activities with electrolyte transport. Morphological studies of the electron microscopists are rapidly being extended to areas involving the renal circulation and its hor monal control. The investigation of the renal handling of a given substance requires consideration not only of renal structure and function but also of the circulation, of metabolic activities, and of the effects on the handling of other substances. The study of fragments of renal tissue is now directly comple mentary to the study of the intact organ in vivo. GLOMERULAR STRUCTURE AND FUNCTIONSome uncertainty remains concerning the initial step in the formation of urine. The applicability of the pore hypothesis to the passage of water and other substances across capillary walls and to the formation of glomerular fluid has been reviewed by Renkin (1). The calculated frequency of the 30-A pores is so small that their detection by electron micrography is considered unlikely. Winne (2) has presented modified derivations for the dilIusion hypothesis and for the pore filtration hypothesis and has proposed criteria for determining which of the processes is operative. In brief, comparison is made of the rate coefficients for transfer by the Fick diffusion equation for concen tration gradients of solute and of the rate coefficients for the thermodynamic and for the Hagen-PoiseuiUe-Pappenheimer equations for transfer of water. Values for the rate coefficients were calculated from older experimental data and the conclusion was reached that the transfers occurred through pores, albeit of a radius or-about 20 A rather than of 30 A. However, the rate coeffi-1 The survey of literature for this review was concluded in June 1963. No attempt has been made to review systematically material published in abstract form nor to provide complete coverage of all full articles.

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“…Eben-104 y.ollinger, Morphologie der Niere hei StofTwechsclstöruiigci Endothel und im Deckepithel selbst gefunden wurden, scheint es sich um die Folge einer Permeation von Eiweißstoffen zu handeln. Im Unterschied zum lichtoptischen Bild erscheint die Basalmembran selbst (Lamina densa) nach den einen Autoren in tak t (Fisher und llellstrom, 1962;Amon und Gayer, 1963), nach anderen (Lee et al. 1961) Noltenius, 1960).…”

Section: Morphologie Der Niere Bei Stoffwechselstörungenunclassified

Morphologie der Niere bei Stoffwechselstörungen

Zollinger¹

1965

Digestion

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Elektronenmikroskopische Untersuchungen �ber den Einflu� der Hyaluronidase auf die Basalmembran der Glomerulumcapillaren mit besonderer Ber�cksichtigung der Permeabilit�tsfrage

Cited by 19 publications

References 46 publications

Acid Mucopolysaccharide and One of Its Glomerular Degrading Enzymes β-Glucuronidase in Experimental and Human Glomerulonephritis

Acid Mucopolysaccharide and One of Its Glomerular Degrading Enzymes β-Glucuronidase in Experimental and Human Glomerulonephritis

Kidney, Water, and Electrolytes

Morphologie der Niere bei Stoffwechselstörungen

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