Elektronenmikroskopische Oberfl�chenabdr�cke und ihr Aufl�sungsverm�gen

Reimer, Ludwig; Schulte, C.

doi:10.1007/bf00622982

Cited by 42 publications

(5 citation statements)

References 34 publications

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“…In an idealized type of object (a sharp step) the resolution of platinum carbon shadowing at the best is in the order of 90 A [9]. No indication of clusters of cholesterolphosphatidylcholine complexes with patches of free phosphatidylcholine, as was detected by other techniques [5 7] was found.…”

Section: The Influence Of Cholesterol On the Crystallization Behavioumentioning

confidence: 90%

The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing

Verkleij

Ververgaert

Kruyff

et al. 1974

Biochimica et Biophysica Acta (BBA) - Biomembranes

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SUMMARYThe crystallization behaviour of bilayers of synthetic phosphatidylcholines in the presence of cholesterol was investigated by freeze fracturing.I. Below the lipid-phase transition, cholesterol is randomly distributed over the lateral plane of a bilayer of the single species dimyristoylphosphatidylcholine, as far as can be detected within the lateral resolution of freeze fracturing.2. in an equimolar mixture of dilauroyl-and dimyristoylphosphatidylcholine, which shows cocrystallization, cholesterol is distributed randomly in the bilayers below the phase-transition temperature.3. In an equimolar mixture of dipalmitoyl and l-palmitoyl-2-oleoylphosphatidylcholine, which shows monotectic behaviour, cholesterol interacts preferentially with the liquid crystalline species 1-palmitoyl-2-oleoylphosphatidylcholine when the other component dipalmitoylphosphatidylcholine passes from the liquid crystalline to the gel state upon cooling the mixture.

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Section: The Influence Of Cholesterol On the Crystallization Behavioumentioning

confidence: 90%

The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing

Verkleij

Ververgaert

Kruyff

et al. 1974

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…Here, we consider polymer replication techniques that are suited for mass production. Polymers have been known for a long time as a replication material with nanometer resolution, although the underlying solidification mechanisms are still being debated . Today, commercial injection molding is routinely used to replicate dense features down to 75 nm in the case of Blu‐Ray discs and 5 nm lateral replication resolution has been achieved .…”

Section: Introductionmentioning

confidence: 99%

Plasmonic Colors: Toward Mass Production of Metasurfaces

Højlund-Nielsen

Clausen

Mäkelä

et al. 2016

Adv Materials Technologies

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Plasmonic metasurface coloration has attracted considerable attention in recent years due to its industrial potential. So far, demonstrations have been limited to small patterned areas fabricated using expensive techniques with limited scalability. This study elevates the technology beyond the common size and volume limitations of nanofabrication and demonstrates aluminum‐coated polymer‐based colored metasurfaces of square‐centimeter size by embossing, injection molding, roll‐to‐roll printing, and film insert molding. Different techniques are compared and the requirements and bottlenecks in terms of master fabrication, replication, metallization, and protection coating for large‐scale production of sub‐wavelength metasurfaces are discussed. Most notably, it is demonstrated that plasmonic metasurface colors are compatible with film insert molding. The results indicate a promising future for plasmonic colors as a viable alternative for decorating mass‐produced polymer parts.

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“…Second, the images represent the distribution of a fairly thick Pt/C deposit (as obtained by the routine freeze-fracture method applied) which reflects more than the relief of the shadowed surface. Rather, the thickness of the deposit and, even more important, self-shadowing and decoration effects limit resolution of relief details to a degree that structural elements smaller than 10 nm may be only vaguely indicated (Reimer and Schulte 1966; Bachmann et al 1985). Third, image analysis reduces random noise in the individual motifs but does not reduce artifacts inherent to the methods of preparation, so that the actual size and form of IMPs or of IMP aggregates will not be precisely reflected by the images obtained.…”

Section: Resultsmentioning

confidence: 99%

Microdomain Arrangement of the SERCA-type Ca²⁺ Pump (Ca²⁺-ATPase) in Subplasmalemmal Calcium Stores of Paramecium Cells

Plattner

Flötenmeyer

Kissmehl

et al. 1999

J Histochem Cytochem.

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SUMMARYWe localized SERCA pumps to the inner region of alveolar sac membranes, facing the cell interior, by combining ultrastructural and biochemical methods. Immunogold labeling largely predominated in the inner alveolar sac region which displayed aggregates of intramembrane particles (IMPs). On image analysis, these represented o ligomeric arrangements of ‫ف‬ 8-nm large IMP subunits, suggesting formation of SERCA aggregates (as known from sarcoplasmic reticulum). We found not only monomers of typical molecular size ( ‫ف‬ 106 kD) but also oligomeric forms on Western blots (using anti-SERCA antibodies, also against endogenous SERCA from alveolar sacs) and on electrophoresis gelautoradiographs of 32 P-labeled phosphoenzyme intermediates. Selective enrichment of SERCA-pump molecules in the inner alveolar sac membrane region may eliminate Ca 2+ after centripetal spread observed during exocytosis activation, while the plasmalemmal Ca 2+ pump may maintain or reestablish [Ca 2+ ] in the narrow subplasmalemmal space between the outer alveolar sac membrane region and the cell membrane. We show for the first time the microzonal arrangement of SERCA molecules in a Ca 2+ store of a secretory system, an intensely discussed issue in stimulus-secretion coupling research.

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Elektronenmikroskopische Oberfl�chenabdr�cke und ihr Aufl�sungsverm�gen

Cited by 42 publications

References 34 publications

The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing

The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing

Plasmonic Colors: Toward Mass Production of Metasurfaces

Microdomain Arrangement of the SERCA-type Ca²⁺ Pump (Ca²⁺-ATPase) in Subplasmalemmal Calcium Stores of Paramecium Cells

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Elektronenmikroskopische Oberfl�chenabdr�cke und ihr Aufl�sungsverm�gen

Cited by 42 publications

References 34 publications

The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing

The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing

Plasmonic Colors: Toward Mass Production of Metasurfaces

Microdomain Arrangement of the SERCA-type Ca2+ Pump (Ca2+-ATPase) in Subplasmalemmal Calcium Stores of Paramecium Cells

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Product

Resources

About

Microdomain Arrangement of the SERCA-type Ca²⁺ Pump (Ca²⁺-ATPase) in Subplasmalemmal Calcium Stores of Paramecium Cells