Elektronenmikroskopische Beobachtungen zur Mastzellendegranulation bei der diffusen Mastocytose des Menschen

Orfanos, Constantin E.; G, Stüttgen

doi:10.1007/bf00489767

Cited by 28 publications

(4 citation statements)

References 33 publications

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“…Our patient was remarkable due to the severity of the cutaneous involvement. Early investigations described the various maturation stages and the degranulation of mast cells in cutaneous mastocytosis under the electron microscope 22 . The fact that our patient had strongly elevated urinary levels of histamine and its metabolite N ‐methylimidazole acetic acid, despite the lack of systemic involvement, underlines the extensive spread of the cutaneous disease.…”

Section: Discussionmentioning

confidence: 54%

“…Early investigations described the various maturation stages and the degranulation of mast cells in cutaneous mastocytosis under the electron microscope. 22 The fact that our patient had strongly elevated urinary levels of histamine and its metabolite N-methylimidazole acetic acid, despite the lack of systemic involvement, underlines the extensive spread of the cutaneous disease. Elevated levels of histamine and its metabolites in 24-h urine samples may be indicative of mastocytosis.…”

Section: Discussionmentioning

confidence: 60%

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Nodular and bullous cutaneous mastocytosis of the xanthelasmoid type: case report

et al. 2001

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Severe generalized nodular and bullous mastocytosis of the xanthelasmoid type is described in a 7-month-old boy. Reddish to yellowish-brown xanthelasmoid papules and nodules first developed in the inguinal region a few weeks after birth and then progressively spread to cover nearly the entire body surface. There was severe pruritus and recurrent episodes of blistering. The diagnosis of cutaneous mastocytosis of the xanthelasmoid type with subepidermal bullae was confirmed by skin biopsies showing solid and deeply penetrating infiltrates of metachromatic mast cells under light and electron microscopy. Systemic involvement of other organs, however, was excluded by bone scintigraphy, abdominal ultrasound, bone marrow aspiration and echocardiography. The extensive skin involvement was reflected in highly elevated urinary levels of histamine (263.4 microg L(-1)) and its metabolite N-methylimidazole acetic acid (20.8 mg L(-1)). The patient was systematically well and received only symptomatic treatment. Over a period of 1 year, the condition gradually improved, and the skin lesions began to flatten and regress.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 60%

Nodular and bullous cutaneous mastocytosis of the xanthelasmoid type: case report

et al. 2001

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“…These nodules, which contain large numbers of mast cells that degranulate upon tactile stimulation, were removed by punch biopsy after stimulation and processed for microscopy . Other studies have been performed on skin obtained by biopsy from a patient with diffuse mastocytosis (36), on nasal and bronchial biopsy cells (43), and on isolated adenoid cells stimulated with calcium ionophore A23187 (4). Several of these analyses utilized primary OS04 fixation (14,18,36), or long periods (hours) of fixation in glutaraldehyde, both of which have resulted in cells with an extracted and distorted cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

“…Other studies have been performed on skin obtained by biopsy from a patient with diffuse mastocytosis (36), on nasal and bronchial biopsy cells (43), and on isolated adenoid cells stimulated with calcium ionophore A23187 (4). Several of these analyses utilized primary OS04 fixation (14,18,36), or long periods (hours) of fixation in glutaraldehyde, both of which have resulted in cells with an extracted and distorted cytoplasm. In unstimulated cells, all authors agree that most of the granules are crystalline but regard the amorphous granules as lipid drop-lets (4,11,18,43).…”

Section: Discussionmentioning

confidence: 99%

Secretion in dissociated human pulmonary mast cells. Evidence for solubilization of granule contents before discharge.

Caulfield¹,

Lewis²,

Hein³

et al. 1980

The Journal of Cell Biology

171

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Mast cells were enzymatically dissociated from human lung fragments that had been sensitized with serum from humans allergic to ragweed and were enriched by isopyknic and velocity gradient sedimentation . Electron microscope examination showed that the mast cells were well preserved at the end of the dissociation and isolation and that the majority of their secretory granules contained crystalline structures . These structures exhibited three patterns-scrolls, gratings, and lattices-which all could be found in the same granule. The period of crystalline structures was found to be bimodal, with maxima at 150 and 75 A. Both periods were observed in gratings that had been tilted and in scrolls that had been cut obliquely, indicating that the various gross patterns are composed of the same basic substructure . After the mast cells were stimulated by rabbit anti-human IgE to release histamine, the contents of the granule were transformed from a crystalline to an amorphous state, and only granules with amorphous contents were seen discharging from the cell. Clusters of intermediate filaments were present around the granules with amorphous contents, both deep in the cytoplasm and discharging at the cell surface . Discharge occurred both by fusion of granule membranes with the plasma membrane and by fusion of granule membranes with other granule membranes that ultimately were continuous with the plasma membrane . After discharge, the granule residue was fibrillar . Cells challenged with anti-human IgE in calcium-free medium neither released histamine nor demonstrated morphologic changes in their granules . We conclude that the crystalline state represents a storage form for materials that are solubilized before fusion of the granule membrane with the plasma membrane and discharge .Tissue mast cells mediate immediate IgE-dependent hypersensitivity reactions through the release of a variety of substances stored in their granules . Detailed morphologic studies of the secretory response of mast cells have, in general, been con-J . CELT . BIOLOGY

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Mastzellenretikulose (Mastocytose)

Grüneberg¹

1969

Nicht Entzündliche Dermatosen II

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Elektronenmikroskopische Beobachtungen zur Mastzellendegranulation bei der diffusen Mastocytose des Menschen

Cited by 28 publications

References 33 publications

Nodular and bullous cutaneous mastocytosis of the xanthelasmoid type: case report

Nodular and bullous cutaneous mastocytosis of the xanthelasmoid type: case report

Secretion in dissociated human pulmonary mast cells. Evidence for solubilization of granule contents before discharge.

Mastzellenretikulose (Mastocytose)

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