Elektronenmikroskopische Befunde am Inselorgan

Gusek, W; Kracht, J

doi:10.1007/978-3-642-86241-0_15

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…Therefore, it has been concluded that hyperinsnlinism in patients with an insulinoma is not due to an excessive production of insulin but to an inability to control its storage and release [12,34]. Reports about ultrastruetural investigations of insulinomas are usually confined to single or few cases [3,14,20,21,25,26,28,41] and, therefore, do not give sufficient information about the broad scale from nearly normal B-cells [3,21,25,26,28] to atypical cells [3,14,20] and the frequency in which they occur in different insulinomas. Of special interest is the correlation of the histology and ultrastrnctm'e of insulinomas with the insulin content of the tissue; this i The expression "insulinoma" is used throughout this article instead of "insulin-producing islet tumour" or "insulin-producing insulinoma" or "insulin-producing pancreatic tumour".…”

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confidence: 99%

Biochemical and morphological investigations of 30 human insulinomas

et al. 1973

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Thirty human insulinomas have been investigated histologically and their immunoreaetive insulin (IRI) content estimated. In most eases immunohistological and ultrastruetural studies were also performed and the percentage of proinsulin-like components (PLC) in the tumour determined. Except for 1 ease the II~I concentration in the tumours was lower (0.01-89.0 U/g) than in the islet tissue. Histologically, immunohistologically and ultrastrueturally a variable number of tumour cells contained few and often no beta-granules, indicating a decreased storage capacity for insulin. This defective storage capacity seems to be the major functional abnormality of insulinoma cells. Ultrastrueturally four types of insulinoma can be distinguished. The ultrastructural diagnosis of an insulinoma can only be made in t?~e I (typicai beta-granules, 13 cases) and type II (typieM and at~2pical granules, 7 eases) but, not in type III (atypical granules only, 4 eases) and type IV (virtually agranular, 4 eases). The type IV tumours had the lowest IRI concentration and did not respond to diazoxide treat-merit. The IRI concentration of the uninvolved pancreas of 19 patients was 2.0~0.2 U/g and in the range of nondiabetic adults.-The percentage PLC in 19 insulinomas was higher (5.3-22%) than in the pancreas of human adults with and without insulinoma (1.7-4.8%). The percentage of PLC in the serum of patients with insulinoma was always higher than in their tumours (33-61%). It is suggested that the higher PLC levels found in the tumour and serum of insulinoma patients are the consequence of the reduced storage capacity of the tumour cells resulting in a rapid passage through the granular route or even a non-granular release of newly synthesized insulin.

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confidence: 99%

Biochemical and morphological investigations of 30 human insulinomas

et al. 1973

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“…The above mentioned (X-cell changes after IPTD first described by v. HOLT and recently confirmed by KRACHT (1959) in the rat, gave rise to divergent opinions. This question must be left open despite the fact that no (X-cell damage was found after IPTD administration in several histological investigations (BENCOSME, MAruz and FREI; DE BASTIANI and GRANATA; MIYAKE also LUNDBAEK and NIELSEN).…”

Section: Methodsmentioning

confidence: 87%

“…The morphological substrate of this augmented islet volume consists of islet enlargement and neo-formation of islets from the epithelia of the small ductules (GEPTS; KRACHT, v. HOLT and v. HOLT; BANDER, HAUSSLER and SCHOLZ; CREUTZFELDT and GEGINAT). Also the possibility of acinar-insular transformation is discussed (GEPTS; KRACHT, 1959, GUSEK and KRACHT;BANDER 1958b). There are said to be acinar cells containing single {1-granules in the marginal zone of rat islets following sulphonylurea medication, according to the electron microscopical investigations of GUSEK and KRACHT (1959b).…”

Section: /'I-cells and Insulin Secretionmentioning

confidence: 99%