Elektronenmikroskopisch-histochemische Untersuchungen zur Darstellung saurer Mucopolysaccharide und sialins�urehaltiger Glycoproteine in Nierenrinde und innerem Nierenmark der Ratte

Nolte, A; Ohkuma, M

doi:10.1007/bf00277783

Cited by 26 publications

(3 citation statements)

References 12 publications

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“…Glomeruli of human and animal kidneys are rich in sialic acid, as indicated by histochemical staining with cationic dyes, such as colloidal iron and alcian blue (1)(2)(3), and by labeling with lectins with specificity for sialic acid (4,5). The anionic groups responsible for these staining properties have been called collectively the "glomerular epithelial polyanion."…”

Section: Introductionmentioning

confidence: 99%

Identification of a major sialoprotein in the glycocalyx of human visceral glomerular epithelial cells.

Kerjaschki¹,

Poczewski²,

Dekan³

et al. 1986

J. Clin. Invest.

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MethodsGlomerular visceral epithelial cells are endowed with a sialic acid-rich surface coat (the "glomerular epithelial polyanion"), which in rat tissue contains the sialoprotein podocalyxin. We have identified a major membrane sialoprotein in human glomeruli that is similar to rat podocalyxin in its sialic acid-dependent binding of wheat germ agglutinin and in its localization on the surface of glomerular epithelial and endothelial cells, as shown by immunoelectron microscopy, using the monoclonal antibody PHM5. Differences in the sialoproteins of the two species are indicated by the discrepancy of their apparent molecular weights in sodium dodecyl sulfate gels, by the lack of cross reactivity of their specific antibodies, and by the lack of homology of their proteolytic peptide maps. It is therefore possible that the human glomerular sialoprotein and rat podocalyxin are evolutionarily distinct, but have similar functions.

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Section: Introductionmentioning

confidence: 99%

Identification of a major sialoprotein in the glycocalyx of human visceral glomerular epithelial cells.

Kerjaschki¹,

Poczewski²,

Dekan³

et al. 1986

J. Clin. Invest.

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“…Glomerular epithelial cells or "podocytes" are endowed with an unusually highly negatively charged cell surface coat, called "epithelial polyanion," which was originally detected using various cationic histochemical stains such as colloidal iron (1)(2)(3)(4), alcian blue (4) and ruthenium red (5), and was identified as sialic acid-rich based on the sensitivity of the staining to digestion with neuraminidase (1)(2)(3)(4). More recently its sialic acid-rich nature was corroborated by the finding that fluorescent-or peroxidase-labeled wheat germ agglutinin (WGA)' binds to the visceral glomerular epithelium in several species (6)(7)(8)(9), and this binding is sensitive to neuraminidase treatment.…”

mentioning

confidence: 99%

Identification and characterization of podocalyxin--the major sialoprotein of the renal glomerular epithelial cell.

Kerjaschki¹,

Sharkey²,

Farquhar³

1984

The Journal of Cell Biology

462

383

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“…One of the components of the glomerulus associated with selective permeability properties of the filter barrier for macromolecules is the sialoglycoprotein termed glomeru lar polyanion (GPA) [13], which is present predominantly along the epithelial side of the glomerular basement membrane. In some forms of the nephrotic state this GPA, which consists of anionic glycoproteins including sialic acid [14][15][16], is strongly reduced, as can be histochemically demonstrated using the colloidal iron staining method [15]. In this paper we present evidence that human or rat lymphoid cells stimulated with Con-A and cultured on cryostat sections of kidney tissue are able to affect the GPA in vitro in contrast to phytohemagglutinin (PHA)-stimulated, or non-stimulated lymphoid cells.…”

Section: Introductionmentioning

confidence: 99%

The Glomerular Polyanion (GPA) of the Rat Kidney

Bakker¹,

Laan²,

Vos³

et al. 1982

Nephron

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Rat peritoneal exudate cells (PEC) or human peripheral blood cells stimulated with mitogens in vitro were cultured in tissue culture chambers mounted on rat or human cryostat kidney sections. After 20 h, the cultures were discontinued and the glomerular polyanion (GPA) of the glomeruli was studied using the colloidal iron stain. The results show that in contrast to PHA-stimulated cells, rat PEC or human blood cells stimulated with Con-A are able to reduce GPA stainability. It was further shown that rat PEC activated with Con-A for 20 or 48 h were able to suppress in vitro lymphocyte transformation of syngeneic blood lymphocytes in a co-culture system following mitogenic stimulation. In view of recent concepts according to which disturbance of T cell function is associated with in vivo loss of GPA in some forms of the nephrotic state, we conclude that investigation into the possible in vivo significance of the present observations is worthwhile.

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Elektronenmikroskopisch-histochemische Untersuchungen zur Darstellung saurer Mucopolysaccharide und sialins�urehaltiger Glycoproteine in Nierenrinde und innerem Nierenmark der Ratte

Cited by 26 publications

References 12 publications

Identification of a major sialoprotein in the glycocalyx of human visceral glomerular epithelial cells.

Identification of a major sialoprotein in the glycocalyx of human visceral glomerular epithelial cells.

Identification and characterization of podocalyxin--the major sialoprotein of the renal glomerular epithelial cell.

The Glomerular Polyanion (GPA) of the Rat Kidney

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