In this work, a theoretical formula based on Equivalent Circuit Model is proposed to describe the influence of frequency on the droplet actuation behavior in reverse electrowetting. To verify the theory, changes of droplet profile are monitored by CCD camera and simulated by COMSOL. It shows that the threshold voltage required for the droplet to start contracting inward increases firstly and then decreases with the increasing frequency. When the frequency is 1kHz, the threshold voltage required for driving droplet reaches the maximum value. Once the droplet begins to move inward, its contact angle increases with the working voltage. As for 4kHz, its contact angle increases linearly from 60° to 154° when U ranges from 16V to 50V, while the droplet remained stationary when U < 16V. The relative experimental results well accord with the theoretical prediction.