Electrothermal atomic absorption spectrometric determination of selenium in biological fluids with rhodium modifier compared with hydride generation atomic spectrometric techniques

Tsalev, Dimiter L.; Lampugnani, Leonardo; D’Ulivo, Alessandro; Petrov, Ivan; Georgieva, R.; Marcucci, Katia; Zamboni, R.

doi:10.1016/s0026-265x(01)00105-9

Cited by 20 publications

(6 citation statements)

References 24 publications

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“…To measure lead (Pb), blood was diluted 10-fold with 0.25% v/v Triton X-100; 10 μL of the sample and 25 μg of (NH 4 ) 2 HPO 4 as a chemical modifier were injected into the THGA, pre-treated with 250 μg of zirconium and 20 μg of iridium [ 43 ]. For selenium (Se), serum was diluted 5-fold with 0.1% v/v Triton X-100 in 0.2% HNO 3 ; 10 μL injections of the sample and 10 μg of rhodium modifier were injected into the THGA; standard addition calibration was applied [ 44 ]. For cadmium (Cd) in urine, the samples were diluted 5-fold with 0.05 v/v Triton X-100 in 0.2% HNO 3 ; 10 μL of the sample and 25 μg of (NH 4 ) 2 HPO 4 as a chemical modifier were injected into the THGA, pre-treated with 250 μg of zirconium and 20 μg of iridium [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

Metals and kidney markers in adult offspring of endemic nephropathy patients and controls: a two-year follow-up study

Karmaus

Dimitrov

Simeonov³

et al. 2008

Environ Health

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Background: The etiology of Balkan Endemic Nephropathy, (BEN), a tubulointerstitial kidney disease, is unknown. Although this disease is endemic in rural areas of Bosnia, Bulgaria, Croatia, Romania, and Serbia, similar manifestations are reported to occur in other regions, for instance Tunisia and Sri Lanka. A number of explanations have been stated including lignites, aristolochic acid, ochratoxin A, metals, and metalloids. Etiologic claims are often based on one or a few studies without sound scientific evidence. In this systematic study, we tested whether exposures to metals (cadmium and lead) and metalloids (arsenic and selenium) are related to Balkan Endemic Nephropathy.

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Section: Methodsmentioning

confidence: 99%

Metals and kidney markers in adult offspring of endemic nephropathy patients and controls: a two-year follow-up study

Karmaus

Dimitrov

Simeonov³

et al. 2008

Environ Health

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“…The trace element content was determined by means of electro-thermal atomic absorption spectrometry (AAS) at wavelength 196.0 nm and band pass 2 nm, upon 4-fold dilution of the samples with 1% v/v Triton X-100 in 0.2% v/v HNO3. A Model 4110 ZL Perkin-Elmer AAS with a transverse heated graphite atomizer (THGA) and longitudinal Zeeman-effect background correction was used for direct AAS measurements; 10 µl of the samples and 10 µl of rhodium modifier (10 µg Rh) were injected into the THGA; pyrolysis and atomization temperatures were 1100 C º and 2100 C º , respectively; an extra cleaning step at 2400 Cº (1+5s) was required; matrix-matched calibrations was applied (Tsalev et al, 2001;Karmaus et al, 2008). The final results were calculated as a mean value of two parallels, each with two replicates.…”

Section: Study Protocol Sample Collection and Analysismentioning

confidence: 99%

Selenium Status in Paroxysmal Atrial Fibrillation

Negreva¹,

Georgiev²,

Vitlianova³

et al. 2015

IJMP

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Background: Selenium deficiency is established in a number of socially important diseases. Data about selenium status in paroxysmal a trial fibrillation (PAF) are lacking. Aim: To study the selenium status in patients with PAF by measuring serum selenium levels during the arrhythmia and after the conversion to sinus rhythm. Methods: Selenium levels were measured in 33 patients (17 male, 16 female; mean age 60.03±1.93) and 33 controls (17 male, 16 female; mean age 59.27±1.72).In patients selenium was examinedupon hospitalization (baseline), on 24 th hour and on 28 th day after sinus rhythm restoration.In controls it was determined once. Selenium was quantified by atomic absorption spectrometry. Results: Mean AF duration up to hospitalization was 8.64±1.03 hours. Baseline selenium values in patients were lower than in controls (0.898±0.025 vs 0.972±0.025 µmol/L, p=0.04). On 24 th hour and 28 th day no significant difference was established (0.938±0.026 vs 0.972±0.025 µmol/L, p=0.35; 0.952±0.023 vs 0.972±0.025 µmol/L, p=0.55, respectively). Conclusion: Selenium deficiency is observed in the early hours of PAF that restores quickly after cardio version. This specific dynamics suggests a close relationship between selenium levels and PAF. Further studies are necessary to position selenium deficiency in the complex system of path physiological mechanisms of PAF manifestation and recurrences.

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“…Generation Atomic Absorption Spectrometry After Preconcentration/ Separation with Modified Organobentonite with Dithizone time is required [9][10][11] . Although HG-AAS methods is powerful analytical tools for determining trace selenium, pre-concentration and separation techniques are still necessary, due to high salt content in addition to the presence of a number of organic constituents 12 .…”

Section: Determination Of Trace Selenium In Urine and Water Samples B...mentioning

confidence: 99%

“…Analysis of real samples: The solid-phase extraction method was applied to the preconcentration/ separation and determination of selenium in the human urine samples and tap water. According to normal method 11 , the samples were pretreatment and the selenium level found in the samples. The results obtained are reported in Table-2.…”

Section: Interferencesmentioning

confidence: 99%

Determination of Trace Selenium in Urine and Water Samples by Hydride Generation Atomic Absorption Spectrometry After Preconcentration/ Separation with Modified Organobentonite with Dithizone

Zhang¹,

Zhao²

2013

Asian J. Chem.

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This paper describes a novel application of modified organobentonite with dithizone (D-O-bentonite) to the preconcentration and separation of selenium using a hydride generation-atomic absorption spectrometry (HG-AAS). A new method for the determination of trace selenium in urine and water has been introduced using a solid-phase extraction agent of D-O-bentonite. Optimal experimental conditions for the adsorption and elution of the selenium, including amount of acid, contact time, eluent volume and co-existing ions have been investigated. The result showed that the selenium could be adsorbed on the D-O-bentonite because of the complexation of dithizone with Se(IV). The adsorbed quantity was affected by the type and amount of acid and contact time. In 50 mL solution, when the dosage of HCl was 20-30 mL and the shaking time was 10 min, the adsorption capacity was 12.09 mg/g. The selenium adsorbed on the D-O-bentonite could be completely eluated by using 5 mLof 1 g/L sodium hydroxide solution. With a preconcentration factor of 100, the detection limit of this method for selenium was 0.0076 µg/L. The proposed solid-phase extraction methodology has been successfully applied to the preconcentration/separation of selenium in the human urine and water samples with satisfied results.

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Electrothermal atomic absorption spectrometric determination of selenium in biological fluids with rhodium modifier compared with hydride generation atomic spectrometric techniques

Cited by 20 publications

References 24 publications

Metals and kidney markers in adult offspring of endemic nephropathy patients and controls: a two-year follow-up study

Metals and kidney markers in adult offspring of endemic nephropathy patients and controls: a two-year follow-up study

Selenium Status in Paroxysmal Atrial Fibrillation

Determination of Trace Selenium in Urine and Water Samples by Hydride Generation Atomic Absorption Spectrometry After Preconcentration/ Separation with Modified Organobentonite with Dithizone

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