Electrosynthesis of polydopamine films - tailored matrices for laccase-based biosensors

Almeida, Luís C.; Correia, Rui D.; Marta, Andreia; Squillaci, Giuseppe; Morana, Alessandra; Cara, Francesco La; Correia, J. Pinto; Viana, Ana S.

doi:10.1016/j.apsusc.2019.03.015

Cited by 38 publications

(41 citation statements)

References 52 publications

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“…4 a). The pristine ePDA film cyclic voltammogram displays a well-defined redox process at E 1/2 = 0.14 V attributed to Q/HQ conversions of the polymeric chains of dopamine, as reported previously 15 , 31 . However, as mentioned above this process may also account for iminoquinones/aminophenols redox conversion, as a result of Schiff base reactions 27 .…”

Section: Resultssupporting

confidence: 83%

“…4 b), confirming the incorporation of ethanolamine in ePDA using either a one-step or a post-modification approach. ePDA spectra displays bands at 1631, 1593, 1512 and 1277 cm −1 , previously reported 15 for this polymer. The two most intense peaks, at 1631 and 1593 cm −1 , correspond to aromatic asymmetric C = C stretching modes of the dihydroxyphenol ring, while less intense aromatic symmetric C=C stretching (1512 cm −1 ) and phenolic C–OH stretching (1277 cm −1 ) modes can also be depicted.…”

Section: Resultssupporting

confidence: 78%

“…It is widely acknowledged that oxygen-driven polymerization of dopamine yields disorganized, poorly conductive and chemically heterogeneous films 11 . Catechol and quinone functional groups coexist, as well as different N-substituted rings 11 , 15 , 16 . Recent studies, revealed that electrosynthesis of polydopamine yield more homogeneous films with better electrochemical properties suitable for electrochemical biosensing interfaces than the standard chemical synthesis, highlighting the great potential of electrochemical methods to better tune the chemical and physical properties of polydopamine 15 , 17 .…”

Section: Introductionmentioning

confidence: 99%

“…Catechol and quinone functional groups coexist, as well as different N-substituted rings 11 , 15 , 16 . Recent studies, revealed that electrosynthesis of polydopamine yield more homogeneous films with better electrochemical properties suitable for electrochemical biosensing interfaces than the standard chemical synthesis, highlighting the great potential of electrochemical methods to better tune the chemical and physical properties of polydopamine 15 , 17 . In addition, it was also demonstrated by combining the electropolymerization processes with gravimetry 18 , that PDA has a much higher affinity for immunoglobulin G (IgG) than poly(pyrrole), due to the presence of the adhesive functionalities.…”

Section: Introductionmentioning

confidence: 99%

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Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces

Almeida

Frade

Correia

et al. 2021

Sci Rep

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We report a straightforward and reproducible electrochemical approach to develop polydopamine-ethanolamine (ePDA-ETA) films to be used as immunosensing interfaces. ETA is strongly attached to polydopamine films during the potentiodynamic electropolymerization of dopamine. The great advantage of the electrochemical methods is to generate the oxidized species (quinones), which can readily react with ETA amine groups present in solution, with the subsequent incorporation of this molecule in the polymer. The presence of ETA and its effect on the electrosynthesis of polydopamine was accessed by cyclic voltammetry, ellipsometry, atomic force microscopy, FTIR and X-ray photoelectron spectroscopy. The adhesive and biocompatible films enable a facile protein linkage, are resilient to flow assays, and display intrinsic anti-fouling properties to block non-specific protein interactions, as monitored by real-time surface plasmon resonance, and confirmed by ellipsometry. Immunoglobulin G (IgG) and Anti-IgG were used in this work as model proteins for the affinity sensor. By using the one-step methodology (ePDA-ETA), the lower amount of immobilized biorecognition element, IgG, compared to that deposited on ePDA or on ETA post-modified film (ePDA/ETA), allied to the presence of ETA, improved the antibody-antigen affinity interaction. The great potential of the developed platform is its versatility to be used with any target biorecognition molecules, allowing both optical and electrochemical detection.

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Section: Resultssupporting

confidence: 83%

Section: Resultssupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces

Almeida

Frade

Correia

et al. 2021

Sci Rep

Self Cite

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“…[34] PDA is a promising active material for bioelectronic platforms due to 1) its high biocompatibility and mild pH and temperature polymerization conditions highly compatible with biomolecules, [41] 2) strong adhesion ability, even in wet conditions, to a wide variety of substrates forming robust films without the need of surface pretreatment, [42] 3) efficient semiconducting properties, [43][44][45][46][47] 4) potential covalent functionalization of PDA films by chemical reactions of catechol moiety on the surface groups of PDA. [38,48] PDA has been already used as efficient adhesive biocompatible polymer for immobilization or encapsulation of enzymes (e.g., laccase, [49] glucosidase, [50] peroxidase [51] ) leading to promising biohybrid materials for catalysis, drug delivery, and biosensing.…”

Section: Q2mentioning

confidence: 99%

Photoelectrodes with Polydopamine Thin Films Incorporating a Bacterial Photoenzyme

Presti

Giangregorio

Ragni

et al. 2020

Adv Elect Materials

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A fabrication strategy of photoactive biohybrid electrodes based on the immobilization of the bacterial reaction center (RC) onto indium tin oxide (ITO) is proposed. The RC is an integral photoenzyme that converts photons into stable charge‐separated states with a quantum yield close to one. The photogenerated electron–hole pair can be eventually exploited, with suitable redox mediators, to produce photocurrents. To this purpose, RC must be effectively anchored on the electrode surface and simple strategies for its stable immobilization ensuring prolonged enzyme photoactivity are strongly desired. In this work, polydopamine (PDA), a polymer reminiscent of the natural melanin, is used to anchor the RC on the electrode surface. PDA is easily prepared in situ by spontaneous polymerization of dopamine in slightly alkaline aerated buffered RC solution. This reaction, carried out in the presence of an ITO substrate dipped into the solution, directly leads to a stable RC‐PDA/ITO photoelectrode with 20 nm film thickness and 50% of fully functional RC occupancy. Photocurrents densities recorded using this photoelectrode are comparable to those obtained with far more sophisticated immobilization techniques. The RC‐PDA films are fully characterized by visible–near‐infrared absorption spectroscopy, ellipsometry, atomic force, and scanning electron microscopies.

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Characterizing Electron Flow through Catechol‐Graphene Composite Hydrogels

Kim

Argenziano

Zhao

et al. 2022

Adv Materials Inter

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and electron-transfer, and then controlling these mechanisms for user-defined purposes. For energy applications (e.g., capacitors and batteries), the focus is on storing large quantities of electrons and controlling their discharge. [1][2][3] For information processing applications (e.g., electronics), [4,5] materials are desired that can gate and rectify electron-flow. [6,7] Other applications focus on materials that offer state-dependent properties (e.g., optoelectronics) [8] or memory (e.g., memristors). [9] With the growing emphasis on safety and sustainability, and the expanding interest in life-science applications, there is an increasing interest in the development of electronic materials that function in aqueous systems. [10] In aqueous systems, electron-transfer is constrained by the solvent and typically occurs through at least two distinctly different mechanisms each of which favors different types of materials. [11][12][13] One electron-transfer mechanism is a metal-like conductivity. [14,15] Carbon-based nanomaterials have been especially important for conferring such conductivity [14,16] with benefits that include enhanced double layer charge storage for energy applications [17][18][19][20][21] and electrocatalytic properties for sensing applications. [22][23][24] A second electron-transfer mechanism involves reduction-oxidation (redox) reactions. Redox polymers offer such properties and have been used in applications that include energy Electronic materials that allow the controlled flow of electrons in aqueous media are required for emerging applications that require biocompatibility, safety, and/or sustainability. Here, a composite hydrogel film composed of graphene and catechol is electrofabricated, and that this composite offers synergistic properties is reported. Graphene confers metal-like conductivity and enables charge-storage through an electrical double layer mechanism. Catechol confers redox-activity and enables charge-storage through a redox mechanism. Importantly, there are two functional populations of catechols: conducting-catechols (presumably in intimate contact with graphene) allow direct electron-transfer; and non-conducting-catechols (presumably physically separated from graphene) require diffusible mediators to enable electrontransfer. Using a variety of spectroelectrochemical measurements, that the capacity of the composite for charge-storage increases in proportion to the extent by which the catechol-groups can undergo redox-state switching is demonstrated. To illustrate the broad relevance of this work, how the redoxstate switching can be related to both the charge storage of energy materials and the memory of molecular electronic materials is discussed. The authors believe this work is significant because it demonstrates that: conducting and redox-active components enable distinctly different mechanisms for chargestorage and electron-transfer; these components act synergistically; and mediators provide unique opportunities to extend the capabilities of electronic materials.

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Electrosynthesis of polydopamine films - tailored matrices for laccase-based biosensors

Cited by 38 publications

References 52 publications

Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces

Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces

Photoelectrodes with Polydopamine Thin Films Incorporating a Bacterial Photoenzyme

Characterizing Electron Flow through Catechol‐Graphene Composite Hydrogels

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