Electrostatic placement of single ferritin molecules

Abstract

We electrostatically placed a single ferritin molecule on a nanometric 3-aminopropyltriethoxysilane (APTES) pattern that was on an oxidized Si substrate. The numerical analysis of the total interaction free energy for ferritin predicted that a quadrilateral array of 15nm diameter APTES nanodisks placed at intervals of 100nm would accommodate a single molecule of ferritin in each disk under a Debye length of 14nm. The experiments we conducted conformed to theoretical predictions and we successfully placed a single ferritin molecule on each ATPES disk without ferritin adsorbing on the SiO2 substrate surface.

Floating nanodot gate memory fabrication with biomineralized nanodot as charge storage node

“…[16][17][18] Artificially biomineralized nanodots are uniform in size and shape due to the restricted inner cavity size and shape of protein shell. Chemical flexibility of protein outer surface made the formation of high-density two dimensional inorganic nanodot arrays 19 and selective deposition 20,21 possible, and vulnerability of protein made the selective removal of organic residues possible. It means that we can produce selectively placed high-density inorganic BND array from protein/BND nanoarchitecture.…”

Protein Based Direct Fabrication of Nano-structures On Si Wafer in Aqueous Solution Bio Nano Process, a Wet Nanotechnology

“…However, electrostatic interaction is a long distance force and, in genral, is thought to be inappropriate for handling nanometric materials. Yoshii and Kumagai solved this issue by carrying out simulation of ferritin-substrate electrostatic interaction and showed that ferritin molecules can be placed on the substrate one by one [7][8][9].…”

Nanoscale device architectures derived from biological assemblies: The case of tobacco mosaic virus and (apo)ferritin

“…6(a) and 6(b)]. 129) SiO 2 substrates and ferritin are negatively charged at neutral pH. Positively charged disks of 15 nm diameter were fabricated by electron beam lithography (EBL), and coated with 3-aminopropyl-triethoxysilane (APTES).…”

“…The nanopattern and size of the positively charged areas can be defined by the electron beam. 129,130) As another placement method for ferritin, diblock copolymers combined with electrochemical modification allow the selective deposition of ferritin in specific nanometer-size areas [ Fig. 6(c)].…”