Electrostatic flocking of chitosan fibres leads to highly porous, elastic and fully biodegradable anisotropic scaffolds

Gossla, Elke; Tonndorf, Robert; Bernhardt, Anne; Kirsten, Martin; Hund, Rolf-Dieter; Aibibu, Dilibar; Cherif, Chokri; Gelinsky, Michael

doi:10.1016/j.actbio.2016.08.022

Cited by 25 publications

(31 citation statements)

References 45 publications

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“…Hence, the flocked chitosan scaffolds prepared in this work are most likely suitable for cartilage TE applications, as stated by Gossla and colleagues as well, who investigated the scaffold type H regarding the proliferation of hMSC and Saos-2 cell lines. 24…”

Section: Resultsmentioning

confidence: 99%

“…Scaffolds were prepared by electrostatic flocking as described by Gossla and colleagues. 24 Flocking from bottom to top was achieved using an electrostatic flocking device (SPG 1000 from Maag Flockmaschinen GmbH, Kusterdingen, Germany), which consisted of a circular top electrode (area 26.42 cm 2 ) and a circular bottom electrode (area 67.93 cm 2 ) made of aluminum alloy and set apart at a distance of 10 cm. The surface of the top electrode was roughened by partial dissolution with 0.1 M NaOH and subsequently thoroughly rinsed with water.…”

Section: Methodsmentioning

confidence: 99%

“…[19][20][21][22][23] Gossla and colleagues recently prepared flocked scaffolds consisting of chitosan only, ensuring full biodegradability. 24 It was found that these scaffolds were suitable for the proliferation of hMSC and Saos-2 cell lines, hence they were proposed as a promising candidate for articular cartilage TE applications.…”

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confidence: 99%

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Factors affecting the mechanical and geometrical properties of electrostatically flocked pure chitosan fiber scaffolds

Tonndorf

Gossla

Kocaman

et al. 2017

Textile Research Journal

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The field of articular cartilage tissue engineering has developed rapidly, and chitosan has become a promising material for scaffold fabrication. For this paper, wet-spun biocompatible chitosan filament yarns were converted into short flock fibers and subsequently electrostatically flocked onto a chitosan substrate, resulting in a pure, highly open, porous, and biodegradable chitosan scaffold. Analyzing the wet-spinning of chitosan revealed its advantages and disadvantages with respect to the fabrication of the fiber-based chitosan scaffolds. The scaffolds were prepared using varying processing parameters and were analyzed in regards to their geometrical and mechanical properties. It was found that the pore sizes were adjustable between 65 and 310 µm, and the compressive strength was in the range 13–57 kPa.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Factors affecting the mechanical and geometrical properties of electrostatically flocked pure chitosan fiber scaffolds

Tonndorf

Gossla

Kocaman

et al. 2017

Textile Research Journal

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“…25,26 Furthermore, one study also shows that stimulation of a immunomodulating pathway can lead to a morphological response in BM-MSCs. 57,58 Thus, culturing hBM-MSCs on biomaterials with specific microstructure 59,60 or stimulation of certain pathways 57 could be used to obtained clinically relevant cell population with high OB differentiation potential or immunomodulatory properties suitable for clinical transplantation.…”

Section: Discussionmentioning

confidence: 99%

Single-cell high-content imaging parameters predict functional phenotype of cultured human bone marrow stromal stem cells

Kowal

Schmal

Halekoh

et al. 2019

Stem Cells Translational Medicine

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Cultured human bone marrow stromal (mesenchymal) stem cells (hBM-MSCs) are heterogenous cell populations exhibiting variable biological properties. Quantitative high-content imaging technology allows identification of morphological markers at a single cell resolution that are determinant for cellular functions. We determined the morphological characteristics of cultured primary hBM-MSCs and examined their predictive value for hBM-MSC functionality. BM-MSCs were isolated from 56 donors and characterized for their proliferative and differentiation potential. We correlated these data with cellular and nuclear morphological features determined by Operetta; a high-content imaging system. Cell area, cell geometry, and nucleus geometry of cultured hBM-MSCs exhibited significant correlation with expression of hBM-MSC membrane markers: ALP, CD146, and CD271. Proliferation capacity correlated negatively with cell and nucleus area and positively with cytoskeleton texture features. In addition, in vitro differentiation to osteoblasts as well as in vivo heterotopic bone formation was associated with decreased ratio of nucleus width to length. Multivariable analysis applying a stability selection procedure identified nuclear geometry and texture as predictors for hBM-MSCs differentiation potential to osteoblasts or adipocytes. Our data demonstrate that by employing a limited number of cell morphological characteristics, it is possible to predict the functional phenotype of cultured hBM-MSCs and thus can be used as a screening test for "quality" of hBM-MSCs prior their use in clinical protocols. K E Y W O R D Scell and nucleus morphology, high-content imaging, human stromal/mesenchymal stem cells, osteoblastic and adipocytic differentiation, proliferation

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“…Coated fiber substrates were placed in the flocking system 5 cm from sieved 4APEGA stock, and various voltages (1, 2, 4, and 6 KV/cm) were applied to produce different flocking concentrations. The applied voltage forces flock particles to propel toward the fiber substrate 33,34 . Postdeposition, samples were exposed to UV light for 5 minutes to permanently bond flocks to the surface by crosslinking.…”

Section: Methodsmentioning

confidence: 99%

Development of a Bioinspired, Self‐Adhering, and Drug‐Eluting Laryngotracheal Patch

et al. 2020

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Objectives/Hypothesis Novel laryngotracheal wound coverage devices are limited by complex anatomy, smooth surfaces, and dynamic pressure changes and airflow during breathing. We hypothesize that a bioinspired mucoadhesive patch mimicking how geckos climb smooth surfaces will permit sutureless wound coverage and also allow drug delivery. Study Design ex‐vivo. Methods Polycaprolactone (PCL) fibers were electrospun onto a substrate and polyethylene glycol (PEG) – acrylate flocks in varying densities were deposited to create a composite patch. Sample topography was assessed with laser profilometry, material stiffness with biaxial mechanical testing, and mucoadhesive testing determined cohesive material failure on porcine tracheal tissue. Degradation rate was measured over 21 days in vitro along with dexamethasone drug release profiles. Material handleability was evaluated via suture retention and in cadaveric larynges. Results Increased flocking density was inversely related to cohesive failure in mucoadhesive testing, with a flocking density of PCL‐PEG‐2XFLK increasing failure strength to 6880 ± 1810 Pa compared to 3028 ± 791 in PCL‐PEG‐4XFLK density and 1182 ± 262 in PCL‐PEG‐6XFLK density. The PCL‐PEG‐2XFLK specimens had a higher failure strength than PCL alone (1404 ± 545 Pa) or PCL‐PEG (2732 ± 840). Flocking progressively reduced composite stiffness from 1347 ± 15 to 763 ± 21 N/m. Degradation increased from 12% at 7 days to 16% after 10 days and 20% after 21 days. Cumulative dexamethasone release at 0.4 mg/cm2 concentration was maintained over 21 days. Optimized PCL‐PEG‐2XFLK density flocked patches were easy to maneuver endoscopically in laryngeal evaluation. Conclusions This novel, sutureless, patch is a mucoadhesive platform suitable to laryngeal and tracheal anatomy with drug delivery capability. Level of Evidence NA Laryngoscope, 131:1958–1966, 2021

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Electrostatic flocking of chitosan fibres leads to highly porous, elastic and fully biodegradable anisotropic scaffolds

Cited by 25 publications

References 45 publications

Factors affecting the mechanical and geometrical properties of electrostatically flocked pure chitosan fiber scaffolds

Factors affecting the mechanical and geometrical properties of electrostatically flocked pure chitosan fiber scaffolds

Single-cell high-content imaging parameters predict functional phenotype of cultured human bone marrow stromal stem cells

Development of a Bioinspired, Self‐Adhering, and Drug‐Eluting Laryngotracheal Patch

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