Electrostatic field can preserve red blood cells in stored blood preparations

Nishiyama, Tomoki; Hayashi, Doubun

doi:10.1007/s00540-006-0460-3

Cited by 4 publications

(2 citation statements)

References 11 publications

(18 reference statements)

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“…HVEF can both accelerate the freezing and thawing speed of meat, and reduce the rate of juice loss, the damage of meat tissue and muscle fibers in the thawing process [11]. Tomoki used electrostatic field to preserve red blood cells, which can improve the survival time of red blood cells and maintain the physiological activity of red blood cells [13]. Therefore, the HVEF has significant effects on the preservation of biological tissues.…”

Section: Introductionmentioning

confidence: 99%

Effect of high-voltage electrostatic field on cryopreservation of human epidermal melanocytes

Cai

Chen

et al. 2021

E3S Web Conf.

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The objective of this research was to evaluate the influence of high-voltage electrostatic field (HVEF) on the freezing of human epidermal melanocytes (HEM) with respect to the degree of cell deformation, survival and proliferation rate after cell resuscitation. As a result, the degree of supercooling is increased by enhancing the strength of the static electric field in the range of 15 kV/m, and the maximum supercooling degree is 7.83±0.05 °C at 15 kV/m. By contrast, the morphology of the electric field assisted freezing cell after resuscitation was significantly improved, and the best electric field strength for cryopreservation is 15 kV/m. The survival rate of human epidermal melanocytes recovered was 88.03%, which was higher than that of the control group. The proliferation rate at 24, 48 and 72 hours are 17%, 28% and 25%, respectively, which are higher than that of the control cells. These findings demonstrate that the freezing HVEF can protect the cell physiological activity, and reduce the freezing damage. Therefore, the optimal HVEF cryopreservation technology be of great significance for the research of tissue engineering in repairing wounds, infections, and promote the development of food, medicine and other fields.

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Section: Introductionmentioning

confidence: 99%

Effect of high-voltage electrostatic field on cryopreservation of human epidermal melanocytes

Cai

Chen

et al. 2021

E3S Web Conf.

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“…Erythrocytes belong to a special class of therapeutic biological products that can only be donated by volunteers and cannot be produced by any other means under the current technological conditions. To maximize the benefit of this hard-toobtain resource in clinical transfusion therapy, it is important to use optimal storage methods to preserve erythrocytes (Hess & Greenwalt, 2002;Kanias & Acker, 2010;Li, Xiong, Wang, Tang, & Wang, 2016;Nishiyama & Hayashi, 2007).…”

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Hypoxic storage of erythrocytes slows down storage lesions and prolongs shelf‐life

Meng

Peng

Zhao³

et al. 2019

Journal Cellular Physiology

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Conventional storage conditions of erythrocytes cause storage lesions. We propose that hypoxic storage conditions, involving removal of oxygen and replacement with helium, the changes in stored erythrocytes under hypoxic condition were observed and assessed. Erythrocytes were divided into two equal parts, then stored in conventional and hypoxic conditions, separately. Blood gas analysis, hemorheology, and hemolysis were performed once a week. Energy metabolism and membrane damage were monitored by enzyme-linked immunosorbent assay. Phosphatidylserine exposure was measured by flow cytometry. P 50 was measured and the oxygen dissociation curve (ODC) plotted accordingly. Erythrocyte morphology was observed microscopically. In the 9th week of storage, the hemolysis of the hypoxia group was 0.7%; lower (p < .05) than that of the control group and still below the threshold of quality requirements. The dissolved oxygen and pO 2 were only 1/4 of that in the control group (p < .01); the adenosine triphosphate, glucose, and lactic acid levels were decreased (p < .05), while the 2,3-diphosphoglycerate levels were increased relative to that in the control group (p < .01). There were no statistically significant differences in membrane damage, deformability, and aggregation between the two groups. In addition, the ODC of the two groups was shifted to the left but this difference was not statistically different. Basically similar to the effect of completely anaerobic conditions. Erythrocytes stored under hypoxic conditions could maintain a relatively stable state with a significant decrease in hemolysis, reduction of storage lesions, and an increase in shelf-life. K E Y W O R D S erythrocyte, hemolysis, hypoxic storage, metabolomics, storage lesions

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Protection of rat artery grafts from tissue damage by voltage-applied supercooling

et al. 2022

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Electrostatic field can preserve red blood cells in stored blood preparations

Abstract: Storing MAP-CRC in an electrostatic field of 500 to 3000 V could decrease hemolysis in the preparation. Considering the lower pH decrease, 500 V might be the field of choice.

Cited by 4 publications

References 11 publications

Effect of high-voltage electrostatic field on cryopreservation of human epidermal melanocytes

Effect of high-voltage electrostatic field on cryopreservation of human epidermal melanocytes

Hypoxic storage of erythrocytes slows down storage lesions and prolongs shelf‐life

Protection of rat artery grafts from tissue damage by voltage-applied supercooling

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