Electrostatic Assembly of Conjugated Polymer Thin Layers on Electrospun Nanofibrous Membranes for Biosensors

Abstract

We report a new fabrication approach to highly sensitive optical sensors by combining the techniques of electrospinning and electrostatic layer-by-layer adsorption. A fluorescent probe, hydrolyzed poly[2-(3-thienyl) ethanol butoxy carbonyl-methyl urethane] (H-PURET), was electrostatically assembled onto the surface of cellulose acetate (CA) electrospun nanofibrous membranes. The fluorescence of these membranes can be quenched by extremely low concentrations (ppb) of methyl viologen (MV 2+) and cytochrome c (cyt c) in aqueous solutions. This high sensitivity is attributed to the high surface-area-to-volume ratio of the electrospun membranes and efficient interaction between the fluorescent conjugated polymer and the analytes.

Eletrofiação de polímeros em solução: parte II: aplicações e perspectivas

“…Em 2004, Liu et al [56] demonstraram a construção de um sensor para amônia com sensibilidade melhorada a partir da manipulação de uma única fibra de poli(anilina) (PANI)/poli(óxido de etileno) (PEO), preparada por eletrofiação. Wang apresentou mais uma inovação na área, com o relato de um sensor fluorimétrico baseado em uma manta de acetato de celulose eletrofiada recoberta com um filme automontado de (PAH/H-PURET) [57] . A sensibilidade neste caso foi menor que o valor anteriormente obtido, porém demonstra a viabilidade da automontagem sobre nanofibras para obtenção de dispositivos.…”

Gallic Acid-loaded Cellulose Acetate Electrospun Nanofibers: Thermal Properties, Mechanical Properties, and Drug Release Behavior

“…[4,5]. These unique properties have triggered a broad range of potential applications, including nanocomposites [6], scaffolds for tissue engineering [7], sensors [8], protective clothing and filtration membranes [9], magneto-responsive fibers [10], superhydrophobic membranes [11], and especially drug delivery [12].…”

Study of Thermally Stimulated Current in Fibrous Poly(DL-Lactic Acid) Films Exhibiting Piezoelectric-Like Behavior

“…The fibers that are ejected from a syringe toward a collector electrode in the presence of a strong electric field are generally gathered in the form of randomly oriented mats or films. These films are viable choices for use in various applications, such as in composite reinforcements [10] , sensors [11] , bio-scaffolds [12] , and in electrochemical photovoltaic electrodes [13] . In the case of our study, it was assumed that the strong electric field that was applied to the PDLLA solution during the fiber formation process resulted in a unique polarization of the PDLLA-fibrous film, which led to a piezoelectric-like effect.…”