Electrostatic assembly of a DNA superparamagnetic nano-tool for simultaneous intracellular delivery and in situ monitoring

Geinguenaud, Frédéric; Souissi, Inès; Fagard, Rémi; Motte, Laurence; Lalatonne, Yoann

doi:10.1016/j.nano.2011.12.010

Cited by 31 publications

(37 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The absorption maxima of dODN and FAM in the UV–Vis spectrum were 260 and 490 nm, respectively, and the absorption maxima at 980, 1129 and 1205 cm −1 in the FTIR spectrum indicate the presence of dODN. The observed absorption maxima are consistent with those reported in literature [24]. In addition to qualitative aspect, the spectral absorption maxima was used to estimate how many dODN molecules were grafted onto PBNPs.…”

Section: Resultssupporting

confidence: 85%

“…The 33-mer DNA drug (dODN) with a sequence 5′CAT TTC CCG TAA ATC GAA GAT TTA CGG GAA ATG 3′ [24], and two kinds of 3′ FAM-labeled dODNs with an 5′ amino and a 5′ thiol linker were synthesized by PURIGO biotechnology Co., Ltd. The human prostate carcinoma 22rv1 cells were obtained from the bioresource collection and research center (BCRC, Taiwan).…”

Section: Methodsmentioning

confidence: 99%

“…The DDS nanoparticles are also promising nuclease-resistance strategies in addition to their cargo roles [21–23] and are thus considered well suited to work as a counter part for DNA drugs. For examples, the Fe 3 O 4 and γ -Fe 2 O 3 nanoparticles are appropriate for DNA drug delivery [24]. Besides, magnetite and maghemite nanoparticles are known for their low cytotoxicity approved by FDA [25], intrinsic magnetic features [26], and facile cellular uptake.…”

Section: Introductionmentioning

confidence: 99%

“…The DNA drug used is a class of decoy oligodeoxynucleotides (dODN) that inhibits the gene expression of STAT3 [24], and two kinds of FAM-labeled dODNs with an amino and a thiol linker are grafted onto 11-mercaptoundecanoic acid (MUA)-functionalized PBNPs separately or together via covalent linkages that result in a DNA drug–PBNPs conjugate (abbreviated as dODN@MUA–PBNPs). The model cancer cells used are human prostate carcinoma 22rv1cells [19].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Prussian blue nanoparticles as nanocargoes for delivering DNA drugs to cancer cells

Wang

Chen

2013

Science and Technology of Advanced Materials

View full text Add to dashboard Cite

We studied the use of Prussian blue nanoparticles (PBNPs) as novel nanocarriers for sending DNA drugs into cancer cells. 11-mercaptoundecanoic acid (MUA) was used to functionalize the surfaces of PBNPs (nanocubes with an average dimension of 75 nm) for subsequent covalent grafting of a 33-mer DNA drug with a FAM reporter at the 3′ end. The PBNPs synthesis and DNA drug conjugation were characterized by transmission electron microscopy (TEM) and Fourier-transform infrared absorption (FTIR), respectively. The drug was a decoy oligodeoxynucleotide (dODN) that inhibits the signal transducer and activator of transcription 3 (STAT3). The DNA–PBNPs drug (dODN@MUA–PBNPs) was delivered into human prostate carcinoma 22rv1 cells by endocytosis in vitro as confirmed by confocal fluorescence microscopy. MTT cell viability assays were carried out to assess the effect of the DNA–PBNPs drug. The results showed that the dODN molecules were successfully conjugated to the MUA modified PBNPs via amide and/or disulfide bond formation and could thus be successfully delivered into the cancer cells. The control experiments showed that the unconjugated dODN molecules were not able to enter the cancer cells no matter whether non-functionalized PBNPs were present or not. It was also found that the DNA-PBNPs drugs were internalized and then distributed homogeneously throughout the cell, including cytoplasmic and nucleic regions, after endocytosis. The cancer cell-killing ability increased with the amount of dODN conjugated on PBNPs and the dosage of DNA–PBNPs drug internalized.

show abstract

Section: Resultssupporting

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prussian blue nanoparticles as nanocargoes for delivering DNA drugs to cancer cells

Wang

Chen

2013

Science and Technology of Advanced Materials

View full text Add to dashboard Cite

show abstract

“…A dODN for the signal transducer and activator of transcription 3 (STAT3) has been successfully adsorbed to the surface of a MNP. [32] The gene coated particles were taken up by the cells, as measured by the response of the cells to the application of magnetic fields, which can be linearly related to the MNP content. MNP uptake was also monitored by the fluorescence of FITC labeled dODNs.…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Theranostic agents for intracellular gene delivery with spatiotemporal imaging

2013

View full text Add to dashboard Cite

Gene therapy is the modification of gene expression to treat a disease. However, efficient intracellular delivery and monitoring of gene therapeutic agents is an ongoing challenge. Use of theranostic agents with suitable targeted, controlled delivery and imaging modalities has the potential to greatly advance gene therapy. Inorganic nanoparticles including magnetic nanoparticles, gold nanoparticles, and quantum dots have been shown to be effective theranostic agents for the delivery and spatiotemporal tracking of oligonucleotides in vitro and even a few cases in vivo. Major concerns remain to be addressed including cytotoxicity, particularly of quantum dots; effective dosage of nanoparticles for optimal theranostic effect; development of real-time in vivo imaging; and further improvement of gene therapy efficacy.

show abstract

Input of Microwaves for Nanocrystal Synthesis and Surface Functionalization Focus on Iron Oxide Nanoparticles

Guénin

Lalatonne

Benyettou

et al. 2013

Microwaves in Nanoparticle Synthesis

View full text Add to dashboard Cite

Electrostatic assembly of a DNA superparamagnetic nano-tool for simultaneous intracellular delivery and in situ monitoring

Cited by 31 publications

References 50 publications

Prussian blue nanoparticles as nanocargoes for delivering DNA drugs to cancer cells

Prussian blue nanoparticles as nanocargoes for delivering DNA drugs to cancer cells

Theranostic agents for intracellular gene delivery with spatiotemporal imaging

Input of Microwaves for Nanocrystal Synthesis and Surface Functionalization Focus on Iron Oxide Nanoparticles

Contact Info

Product

Resources

About