Electrospun silk nanofibers improve differentiation potential of human induced pluripotent stem cells to insulin producing cells

Enderami, Seyed Ehsan; Ahmadi, Seyedeh Fatemeh; Mansour, Reyhaneh Nassiri; Abediankenari, Saeid; Ranjbaran, Hossein; Mossahebi-Mohammadi, Majid; Salarinia, Reza; Mahboudi, Hossein

doi:10.1016/j.msec.2019.110398

Cited by 15 publications

(5 citation statements)

References 34 publications

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“…As other NF scaffold, SF was constructed to simulate the pancreatic microenvironment for differentiation of iPSCs into IPCs which significantly evoked proliferation of the hiPSCs and its differentiation potential into IPCs [122]. Furthermore, IPC differentiation potency of hAD-SCs was examined on a hybrid NF scaffold composed of SF and PES polymers.…”

Section: Cell Delivery and Tissue Engineeringmentioning

confidence: 99%

Nanofiber-based systems intended for diabetes

et al. 2021

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Diabetic mellitus (DM) is the most communal metabolic disease resulting from a defect in insulin secretion, causing hyperglycemia by promoting the progressive destruction of pancreatic β cells. This autoimmune disease causes many severe disorders leading to organ failure, lower extremity amputations, and ultimately death. Modern delivery systems e.g., nanofiber (NF)-based systems fabricated by natural and synthetic or both materials to deliver therapeutics agents and cells, could be the harbinger of a new era to obviate DM complications. Such delivery systems can effectively deliver macromolecules (insulin) and small molecules. Besides, NF scaffolds can provide an ideal microenvironment to cell therapy for pancreatic β cell transplantation and pancreatic tissue engineering. Numerous studies indicated the potential usage of therapeutics/cells-incorporated NF mats to proliferate/regenerate/remodeling the structural and functional properties of diabetic skin ulcers. Thus, we intended to discuss the aforementioned features of the NF system for DM complications in detail. Graphic abstract

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Section: Cell Delivery and Tissue Engineeringmentioning

confidence: 99%

Nanofiber-based systems intended for diabetes

et al. 2021

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“…They can significantly improve the function of target organs by regulating apoptosis and inflammation and promoting proliferation. 8,9 Adult stem cells can be auto-grafted without ethical or legal issues, and they can overcome mutations and other adverse effects. 10 The commonly used adult stem cells include bone marrow mesenchymal stem cells (BMSCs), endometrial mesenchymal stem cells (EMSCs), and adipose mesenchymal stem cells (ADSCs).…”

Section: Introductionmentioning

confidence: 99%

Adipose mesenchymal stem cell-based tissue engineering mesh with sustained bFGF release to enhance tissue repair

Guo

Jia

et al. 2022

Biomater. Sci.

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“…Besides, the osteogenic differentiation capacity of mesenchymal stem cells (MSCs) was promoted when MSCs were cultured as spheroids or in various 3D environments[ 5 , 6 ]. Therefore, the 3D cell culture methods are potential tools for anti-cancer drug screening[ 7 ], tissue engineering[ 8 ], and regenerative medicine[ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Changes of cell membrane fluidity for mesenchymal stem cell spheroids on biomaterial surfaces

Wong¹,

Han²,

Hsu³

2022

WJSC

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BACKGROUND The therapeutic potential of mesenchymal stem cells (MSCs) in the form of three-dimensional spheroids has been extensively demonstrated. The underlying mechanisms for the altered cellular behavior of spheroids have also been investigated. Cell membrane fluidity is a critically important physical property for the regulation of cell behavior, but it has not been studied for the spheroid-forming cells to date. AIM To explore the association between cell membrane fluidity and the morphological changes of MSC spheroids on the surface of biomaterials to elucidate the role of membrane fluidity during the spheroid-forming process of MSCs. METHODS We generated three-dimensional (3D) MSC spheroids on the surface of various culture substrates including chitosan (CS), CS-hyaluronan (CS-HA), and polyvinyl alcohol (PVA) substrates. The cell membrane fluidity and cell morphological change were examined by a time-lapse recording system as well as a high-resolution 3D cellular image explorer. MSCs and normal/cancer cells were pre-stained with fluorescent dyes and co-cultured on the biomaterials to investigate the exchange of cell membrane during the formation of heterogeneous cellular spheroids. RESULTS We discovered that vesicle-like bubbles randomly appeared on the outer layer of MSC spheroids cultured on different biomaterial surfaces. The average diameter of the vesicle-like bubbles of MSC spheroids on CS-HA at 37 °C was approximately 10 μm, smaller than that on PVA substrates (approximately 27 μm). Based on time-lapse images, these unique bubbles originated from the dynamic movement of the cell membrane during spheroid formation, which indicated an increment of membrane fluidity for MSCs cultured on these substrates. Moreover, the membrane interaction in two different types of cells with similar membrane fluidity may further induce a higher level of membrane translocation during the formation of heterogeneous spheroids. CONCLUSION Changes in cell membrane fluidity may be a novel path to elucidate the complicated physiological alterations in 3D spheroid-forming cells.

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Electrospun silk nanofibers improve differentiation potential of human induced pluripotent stem cells to insulin producing cells

Cited by 15 publications

References 34 publications

Nanofiber-based systems intended for diabetes

Nanofiber-based systems intended for diabetes

Adipose mesenchymal stem cell-based tissue engineering mesh with sustained bFGF release to enhance tissue repair

Changes of cell membrane fluidity for mesenchymal stem cell spheroids on biomaterial surfaces

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