Electrospun silk fibroin scaffolds coated with reduced graphene promote neurite outgrowth of PC-12 cells under electrical stimulation

Aznar-Cervantes, Salvador D.; Pagán, Ana; Martínez, José G.; Bernabeu-Esclapez, A.; Otero, Toribio F.; Meseguer‐Olmo, Luis; Paredes, J.I.; Cenis, J. L.

doi:10.1016/j.msec.2017.05.055

Cited by 76 publications

(71 citation statements)

References 66 publications

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“…Graphene-based nanomaterials, including graphene, graphene oxide (GO) and reduced graphene oxide (rGO), have attracted increasing attention because of their excellent conductivity 16,17 , making them promising candidates for fabricating highly conductive composite biomaterials in a low concentration 18,19 . Recent studies have also revealed that the direct absorption of GO onto biomaterials enabled the realization of high conductivity with the extremely low mass of GO [20][21][22] . In these studies, microscaled fibrous matrices (4-170 μm) were employed as substrates, whose high porosities and large surface areas allowed GO platelets to fully infiltrate into pores and absorb onto fiber surfaces to form an intact and conductive GO nanolayer.…”

Section: Introductionmentioning

confidence: 99%

Reduced graphene oxide functionalized nanofibrous silk fibroin matrices for engineering excitable tissues

et al. 2018

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Tissue engineering has provided an alternative strategy for the regeneration of functional tissues for drug screening and disease intervention. The central challenge in the development of mature and functional excitable tissues is to design and construct advanced conductive biomaterials that can guide cells to form electrically interconnected networks. The objective of this study was to develop reduced graphene oxide modified silk nanofibrous biomaterials with controllable surface deposition on the nanoscale. A vacuum filtration system was applied to attain reduced graphene oxide nanolayer deposition. The results demonstrate that with this method, a uniform and compact reduced graphene oxide nanolayer was formed, and the conductivity and nanofibrous morphology of the materials was well controlled. The composite nanofibrous scaffolds were applied for the engineering of cardiac tissues and demonstrated a great ability to promote tissue formation and functions, including the expression of cardiac-specific proteins, the formation of sarcomeric structures and gap junctions, and tissue contraction. External electrical stimulation further enhanced the maturation level of cardiac tissues cultured on these conductive scaffolds. All these results demonstrated the great potential of reduced graphene oxide functionalized silk biomaterials fabricated using our method for recapitulating electrical microenvironments for the regeneration of functional excitable tissues.

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Section: Introductionmentioning

confidence: 99%

Reduced graphene oxide functionalized nanofibrous silk fibroin matrices for engineering excitable tissues

et al. 2018

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“…12 Additionally, SF electrospinning is commonly combined with additional functionalizing methods. Such as those involving the incorporation of molecules of interest (growth factors among others), 13 the utilization of different SF blends in combination with other polymers, 14 the adsorption of conductive polymers on its surface 15,16 or even the combination with nanoparticles designed to catalyze the degradation of chemical warfare agents. 6 Several research groups have also combined sericulture and TiO 2 nanoparticles in works of diverse nature.…”

Section: Introductionmentioning

confidence: 99%

Electrospun silk fibroin/TiO₂ mats. Preparation, characterization and efficiency for the photocatalytic solar treatment of pesticide polluted water

et al. 2020

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“…Graphene‐based materials (GBM) hold promise in drug delivery, gene delivery, photothermal therapy, and tissue engineering . This relates to unique properties including their single layer, 2D nature, conferring a high surface area, and mechanical strength.…”

Section: Introductionmentioning

confidence: 99%

Exfoliation in Endotoxin‐Free Albumin Generates Pristine Graphene with Reduced Inflammatory Properties

et al. 2018

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mechanical strength. Within the GBMs, pristine graphene (pG) presents key advantages; its virtually defect-free surface enhances its mechanical properties and the absence of oxygen groups increases its electrical and optical conductivity compared to functionalized graphene, [7] presenting opportunities to develop new biomedical devices. Moreover, since no additional chemical modifications are required, the use of pG would have a positive environmental and economic impact over functionalized graphenes, such as graphene oxide (GO). Nevertheless, pG has been less studied in a biological context, due to its intrinsic hydrophobicity, restricting dispersibility and stability in aqueous solution, unless potentially toxic stabilizers are added. Thus, the use of biocompatible dispersants is crucial.We currently lack a complete understanding of the toxic and inflammatory effects of GBMs and conflicting results have been reported. While the toxicity of other graphene-based materials (e.g., GO, rGO), has been extensively studied, [1,[8][9][10] limited work has been done on pristine graphene. [11][12][13] Studies highlight key roles for factors such as lateral size (small vs large), [10] surface functionalization (e.g., GO vs pG), [12,14] purity [15] and hydrophobicity [16] on GBM-cell interaction, thus it is vital to address the intrinsic toxicological effects of pG and elucidate its immunomodulatory properties in order to assess its specific potential as a material for biomedical applications. According Graphene-based materials are under consideration as a new generation of biomaterials for medical applications, owning to their distinctive physicochemical properties. A major obstacle to clinical translation of novel biomaterials is the presence of residual endotoxin contaminants that can trigger deleterious inflammatory responses and unrelated immunomodulatory effects. To overcome this challenge, a liquid exfoliation methodology to prepare endotoxin-free bovine serum albumin (BSA)-exfoliated pristine graphene (EF-Gr) was developed and compared with conventional BSA-exfoliated graphene (C-Gr) and sodium cholate-exfoliated graphene (SC-Gr). In contrast to SC-Gr, protein-exfoliated graphene exhibits low toxicity. Moreover, BSAexfoliated EF-Gr does not trigger cytokine secretion or increase costimulatory molecule expression in dendritic cells (DCs) or macrophages; however it retains the ability to activate the NLRP3 inflammasome in DCs primed withToll-like receptor (TLR) agonists. The results suggest that some reported immunostimulatory effects of graphene preparations may have resulted from contaminants in the formulations. When injected into mice, the inflammatory response induced is comparable in magnitude to that induced by the safe and effective vaccine adjuvant alum. Overall, the study demonstrates the feasibility of producing endotoxin-free pristine graphene that facilitates assessment of the potential of pristine graphene for novel biomedical applications without the confounding effects of toxic and inflammatory conta...

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Electrospun silk fibroin scaffolds coated with reduced graphene promote neurite outgrowth of PC-12 cells under electrical stimulation

Cited by 76 publications

References 66 publications

Reduced graphene oxide functionalized nanofibrous silk fibroin matrices for engineering excitable tissues

Reduced graphene oxide functionalized nanofibrous silk fibroin matrices for engineering excitable tissues

Electrospun silk fibroin/TiO₂ mats. Preparation, characterization and efficiency for the photocatalytic solar treatment of pesticide polluted water

Exfoliation in Endotoxin‐Free Albumin Generates Pristine Graphene with Reduced Inflammatory Properties

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Electrospun silk fibroin scaffolds coated with reduced graphene promote neurite outgrowth of PC-12 cells under electrical stimulation

Cited by 76 publications

References 66 publications

Reduced graphene oxide functionalized nanofibrous silk fibroin matrices for engineering excitable tissues

Reduced graphene oxide functionalized nanofibrous silk fibroin matrices for engineering excitable tissues

Electrospun silk fibroin/TiO2 mats. Preparation, characterization and efficiency for the photocatalytic solar treatment of pesticide polluted water

Exfoliation in Endotoxin‐Free Albumin Generates Pristine Graphene with Reduced Inflammatory Properties

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Product

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Electrospun silk fibroin/TiO₂ mats. Preparation, characterization and efficiency for the photocatalytic solar treatment of pesticide polluted water