Electrospun Scaffolds in Tendons Regeneration: a review

Gesù, Roberto Di; Amato, Giovanni; Gottardi, Riccardo

doi:10.32098/mltj.04.2019.02

Cited by 6 publications

(4 citation statements)

References 98 publications

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“…Electrospinning, one of the most suitable and promising techniques available to produce scaffolds for tissue engineering applications [ 10 , 11 , 12 , 13 , 14 ], aims at obtaining fibrous matrices with biomimetic micro- to nanoscale diameters resembling the architecture of the native extracellular matrix (ECM) [ 14 , 15 , 16 ]. Scaffolds with adjustable geometry, surface chemistry, and mechanical properties can be obtained by controlling the electrospinning process parameters making it a versatile technique in this field [ 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential

et al. 2020

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Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct.

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Section: Introductionmentioning

confidence: 99%

Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential

et al. 2020

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“…Chemically, hydrogels are polymeric compounds composed of a main chain (backbone) that can be used as an anchorage point for several bioactive molecules or secondary polymer molecules (side chains) characterized by different chemical–physical properties. Hydrogels are classified as natural, if polymers derive from a natural source (e.g., collagen, fibrin, hyaluronic acid), or synthetic/semi-synthetic, if they derive, completely or partially, from chemical reactions [ 98 ]. Such chemical heterogeneity enables a wide range of structural modifications, and thus modulation of biological responses from embedded cells.…”

Section: Toward Improved Human Ipsc-based Hepatobiliary Modelsmentioning

confidence: 99%

Generation of Hepatobiliary Cell Lineages from Human Induced Pluripotent Stem Cells: Applications in Disease Modeling and Drug Screening

Pasqua

Gesù

Chinnici

et al. 2021

IJMS

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The possibility to reproduce key tissue functions in vitro from induced pluripotent stem cells (iPSCs) is offering an incredible opportunity to gain better insight into biological mechanisms underlying development and disease, and a tool for the rapid screening of drug candidates. This review attempts to summarize recent strategies for specification of iPSCs towards hepatobiliary lineages —hepatocytes and cholangiocytes— and their use as platforms for disease modeling and drug testing. The application of different tissue-engineering methods to promote accurate and reliable readouts is discussed. Space is given to open questions, including to what extent these novel systems can be informative. Potential pathways for improvement are finally suggested.

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“…10–20 kV) electrostatic field, which are randomly collected on the “ground” electrode substrate (usually stainless steel or aluminum plate) [ 20 ]. The resulting non-woven fibrous and biocompatible structure has a high surface to volume ratio with porosity up to 97% [ 21 ], promoting effective cell adhesion and proliferation [ 22 ]. During electrospinning, intra- and intermolecular bonds between fibers are practically not formed, which leads to a loss of structural stability of the scaffold in an aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Natural and Synthetic Polymer Scaffolds Comprising Upconversion Nanoparticles as a Bioimaging Platform for Tissue Engineering

Trifanova

Khvorostina²,

Mariyanats³

et al. 2022

Molecules

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Modern biocompatible materials of both natural and synthetic origin, in combination with advanced techniques for their processing and functionalization, provide the basis for tissue engineering constructs (TECs) for the effective replacement of specific body defects and guided tissue regeneration. Here we describe TECs fabricated using electrospinning and 3D printing techniques on a base of synthetic (polylactic-co-glycolic acids, PLGA) and natural (collagen, COL, and hyaluronic acid, HA) polymers impregnated with core/shell β-NaYF4:Yb3+,Er3+/NaYF4 upconversion nanoparticles (UCNPs) for in vitro control of the tissue/scaffold interaction. Polymeric structures impregnated with core/shell β-NaYF4:Yb3+,Er3+/NaYF4 nanoparticles were visualized with high optical contrast using laser irradiation at 976 nm. We found that the photoluminescence spectra of impregnated scaffolds differ from the spectrum of free UCNPs that could be used to control the scaffold microenvironment, polymer biodegradation, and cargo release. We proved the absence of UCNP-impregnated scaffold cytotoxicity and demonstrated their high efficiency for cell attachment, proliferation, and colonization. We also modified the COL-based scaffold fabrication technology to increase their tensile strength and structural stability within the living body. The proposed approach is a technological platform for “smart scaffold” development and fabrication based on bioresorbable polymer structures impregnated with UCNPs, providing the desired photoluminescent, biochemical, and mechanical properties for intravital visualization and monitoring of their behavior and tissue/scaffold interaction in real time.

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Electrospun Scaffolds in Tendons Regeneration: a review

Cited by 6 publications

References 98 publications

Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential

Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential

Generation of Hepatobiliary Cell Lineages from Human Induced Pluripotent Stem Cells: Applications in Disease Modeling and Drug Screening

Natural and Synthetic Polymer Scaffolds Comprising Upconversion Nanoparticles as a Bioimaging Platform for Tissue Engineering

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