Electrospun poly(vinylidene fluoride-trifluoroethylene)/zinc oxide nanocomposite tissue engineering scaffolds with enhanced cell adhesion and blood vessel formation

Augustine, Robin; Dan, Pan; Sosnik, Alejandro; Kalarikkal, Nandakumar; Tran, Nguyen; Vincent, Brice; Thomas, Sabu; Menu, Patrick; Rouxel, Didier

doi:10.1007/s12274-017-1549-8

Cited by 160 publications

(111 citation statements)

References 64 publications

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“…As evident from the previous reports, ROS generated by TNR might have played a major role in angiogenesis; however, its underlying mechanisms remain not well understood. Studies using other metal oxide nanoparticles such as zinc oxide and europium hydroxide support our present findings. In addition, we performed C‐reactive protein (CRP) assay to determine systemic inflammatory response if any caused by implanted meshes.…”

Section: Resultssupporting

confidence: 90%

Titanium Nanorods Loaded PCL Meshes with Enhanced Blood Vessel Formation and Cell Migration for Wound Dressing Applications

Augustine

Hasan

Patan

et al. 2019

Macromolecular Bioscience

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Proper management of nonhealing wounds is an imperative clinical challenge. For the effective healing of chronic wounds, suitable wound coverage materials with the capability to accelerate cell migration, cell proliferation, angiogenesis, and wound healing are required to protect the healing wound bed. Biodegradable polymeric meshes are utilized as effective wound coverage materials to protect the wounds from the external environment and prevent infections. Among them, electrospun biopolymeric meshes have got much attention due to their extracellular matrix mimicking morphology, ability to support cell adhesion, and cell proliferation. Herein, electrospun nanocomposite meshes based on polycaprolactone (PCL) and titanium dioxide nanorods (TNR) are developed. TNR incorporated PCL meshes are fabricated by electrospinning technique and characterized by scanning electron microscopy, energy dispersive X‐ray spectroscopy, Fourier transform infrared spectroscopy (FTIR) analysis, and X‐Ray diffraction (XRD) analysis. In vitro cell culture studies, in ovo angiogenesis assay, in vivo implantation study, and in vivo wound healing study are performed. Interestingly, obtained in vitro and in vivo results demonstrated that the presence of TNR in the PCL meshes greatly improved the cell migration, proliferation, angiogenesis, and wound healing. Owing to the above superior properties, they can be used as excellent biomaterials in wound healing and tissue regeneration applications.

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Section: Resultssupporting

confidence: 90%

Titanium Nanorods Loaded PCL Meshes with Enhanced Blood Vessel Formation and Cell Migration for Wound Dressing Applications

Augustine

Hasan

Patan

et al. 2019

Macromolecular Bioscience

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“…Live/dead studies showed no differences between ZnO composite scaffolds and PCL control. Augustine et al () reported that 4% ZnO/PVDF‐TrFE was slightly cytotoxic for MSCs and endothelial cells. Different cytotoxicity levels could be due to the properties of the nanoparticles, particle size, purity and size distribution (Sahu, Kannan, Tailang, & Vijayaraghavan, ) in addition to the polymer (Sukhanova et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Cells on 10% ZnO demonstrated the highest VEGF gene expression in GM and OS media at Day 21. PVDF/ZnO scaffolds seeded with MSCs enhanced angiogenesis when implanted subcutaneously in the abdominal region rats (Augustine et al, ).…”

Section: Discussionmentioning

confidence: 99%

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Biodegradable zinc oxide composite scaffolds promote osteochondral differentiation of mesenchymal stem cells

Khader

Arinzeh

2019

Biotech & Bioengineering

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Osteoarthritis (OA) involves the degeneration of articular cartilage and subchondral bone. The capacity of articular cartilage to repair and regenerate is limited. A biodegradable, fibrous scaffold containing zinc oxide (ZnO) was fabricated and evaluated for osteochondral tissue engineering applications. ZnO has shown promise for a variety of biomedical applications but has had limited use in tissue engineering. Composite scaffolds consisted of ZnO nanoparticles embedded in slow degrading, polycaprolactone to allow for dissolution of zinc ions over time. Zinc has well‐known insulin‐mimetic properties and can be beneficial for cartilage and bone regeneration. Fibrous ZnO composite scaffolds, having varying concentrations of 1–10 wt.% ZnO, were fabricated using the electrospinning technique and evaluated for human mesenchymal stem cell (MSC) differentiation along chondrocyte and osteoblast lineages. Slow release of the zinc was observed for all ZnO composite scaffolds. MSC chondrogenic differentiation was promoted on low percentage ZnO composite scaffolds as indicated by the highest collagen type II production and expression of cartilage‐specific genes, while osteogenic differentiation was promoted on high percentage ZnO composite scaffolds as indicated by the highest alkaline phosphatase activity, collagen production, and expression of bone‐specific genes. This study demonstrates the feasibility of ZnO‐containing composites as a potential scaffold for osteochondral tissue engineering.

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“…A tissue engineering blood vessel should be biocompatible, having appropriate mechanical properties and its availability in a variety of size for grafting applications. It is now an actively investigated field for small diameter vascular graft . Recently, the vascular transplantation has been commonly used for the treatment of cardiovascular or vascular diseases by knitted tubular fabrication system and some scaffolds fabricated by the electrospinning of synthetic polymers such as polylactide, poly(lactide‐ co ‐glycolide), poly(ε‐caprolactone), collagen, and silk fibroin, but there was no good elongation, breaking strength, and dimensional stability .…”

Section: Introductionmentioning

confidence: 99%

In vitro assessment of dual‐network electrospun tubes from poly(1,4 cyclohexane dimethylene isosorbide terephthalate)/PVA hydrogel for blood vessel application

Khan

Kharaghani

Nishat

et al. 2018

J of Applied Polymer Sci

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The fabrication of artificial blood vessel remains an ongoing challenge for cardiovascular tissue engineering. Full biocompatibility, proper physiological, and immediate availability have emerged as central issues. To address these issues, the dual-network composite scaffolds were fabricated by coating the electrospun nanofibers-based tubes with poly(vinyl alcohol) (PVA) hydrogel, which could increase the cell viability and show the potential for controlling the composition, structure, and mechanical properties of scaffolds. Herein, the tubular scaffolds having an inner diameter of 2 mm, were composed with poly(1,4 cyclohexane dimethylene isosorbide terephthalate)/PVA. The morphology examination showed that tubular structure was dimensionally stable and suitable for an artificial blood vessel. Fourier transform infrared spectra, wetting behavior, stress-strain behavior, and Thiazolyl Blue Tetrazolium Bromide (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) analysis also showed that the composite scaffolds have good chemical interactions between poly(1,4 cyclohexane dimethylene isosorbide terephthalate) (PICT) and PVA, blended PICT/PVA tubes showed the appropriate wetting behavior, it achieved the appropriate breaking strength and adequate pliability up to 47.5% and in vitro assessment showed that blended PICT/PVA scaffolds have the appropriate cell viability and nontoxic, respectively. On the basis of characterizations results, it was concluded that resultant scaffolds would be addressed to fulfill the requirements such as biocompatibility, dimensional stability, adequate elongation, breaking strength, immediate availability, and proper for physiologically.

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Electrospun poly(vinylidene fluoride-trifluoroethylene)/zinc oxide nanocomposite tissue engineering scaffolds with enhanced cell adhesion and blood vessel formation

Cited by 160 publications

References 64 publications

Titanium Nanorods Loaded PCL Meshes with Enhanced Blood Vessel Formation and Cell Migration for Wound Dressing Applications

Titanium Nanorods Loaded PCL Meshes with Enhanced Blood Vessel Formation and Cell Migration for Wound Dressing Applications

Biodegradable zinc oxide composite scaffolds promote osteochondral differentiation of mesenchymal stem cells

In vitro assessment of dual‐network electrospun tubes from poly(1,4 cyclohexane dimethylene isosorbide terephthalate)/PVA hydrogel for blood vessel application

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