Electrospun PLGA–silk fibroin–collagen nanofibrous scaffolds for nerve tissue engineering

Abstract

Electrospun nanofibrous scaffolds varying different materials are fabricated for tissue engineering. PLGA, silk fibroin, and collagen-derived scaffolds have been proved on good biocompatibility with neurons. However, no systematic studies have been performed to examine the PLGA-silk fibroin-collagen (PLGA-SF-COL) biocomposite fiber matrices for nerve tissue engineering. In this study, different weight ratio PLGA-SF-COL (50:25:25, 30:35:35) scaffolds were produced via electrospinning. The physical and mechanical properties were tested. The average fiber diameter ranged from 280 + 26 to 168 + 21 nm with high porosity and hydrophilicity; the tensile strength was 1.76 ± 0.32 and 1.25 ± 0.20 Mpa, respectively. The results demonstrated that electrospinning polymer blending is a simple and effective approach for fabricating novel biocomposite nanofibrous scaffolds. The properties of the scaffolds can be strongly influenced by the concentration of collagen and silk fibroin in the biocomposite. To assay the cytocompatibility, Schwann cells were seeded on the scaffolds; cell attachment, growth morphology, and proliferation were studied. SEM and MTT results confirmed that PLGA-SF-COL scaffolds particularly the one that contains 50% PLGA, 25% silk fibroin, and 25% collagen is more suitable for nerve tissue engineering compared to PLGA nanofibrous scaffolds.

A biocomposite of collagen nanofibers and nanohydroxyapatite for bone regeneration

“…For that reason, synthetic polymers such poly-glycolic acid (PGA), polyL-lactic acid (PLLA), polylactic-coglycolic acid (PLGA) or polycaprolactone (PCL) are often added to the collagen solution [20][21][22]. However, the che-micals (additives, traces of catalysts, inhibitors) or mono-mers (glycolic acid, lactic acid) released from polymer degradation may induce local and systemic host reactions that may cause clinical problems [23,24].…”

Functional nanofibers for tissue engineering applications

“…Randomly oriented fi brous scaffolds have been modifi ed via surface functionalization, encapsulation of growth factors and blends of natural polymers to construct scaffolds more suitable for nerve regeneration compared to their respective synthetic polymer controls (Koh et al , 2008; © Woodhead Publishing Limited, 2012 Nisbet et al , 2008;Prabhakaran et al , 2008Prabhakaran et al , , 2009aAlvarez-Perez et al , 2010;Liu et al , 2011;Wang et al , 2011b). However, since axonal guidance is a key factor in nerve regeneration, the majority of electrospun scaffolds intended for nerve tissue engineering consist of aligned nanofi bers to direct axonal growth.…”

Electrospun Poly(lactide-co-glycolide-co-3(S)-methyl-morpholine-2,5-dione) Nanofibrous Scaffolds for Tissue Engineering

“…The ideal materials for vascular tissue engineering should have excellent biocompatibility, good mechanical properties, and appropriate degradation behavior [23]. Biodegradable polymers, such as poly(L-lactic acid) (PLLA), poly(glycolic acid) (PGA) and poly(lactic-co-glycolic acid) (PLGA), have drawn a lot of attention in research because of their well-characterized biodegradable properties [24][25][26][27][28].…”