Electrospun plant mucilage nanofibers as biocompatible scaffolds for cell proliferation

Ureña-Saborío, Hilary; Alfaro‐Viquez, Emilia; Esquivel‐Alvarado, Daniel; Madrigal‐Carballo, Sergio; Gunasekaran, Sundaram

doi:10.1016/j.ijbiomac.2018.04.129

Cited by 31 publications

(26 citation statements)

References 26 publications

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“…In particular, a 40% increase in oligodendrocyte differentiation and a 20% increase in neuronal differentiation were found using fibers with average fiber diameter of 283 nm and 749 nm, respectively, in comparison to the controls of tissue culture polystyrene substrates. Others have demonstrated that large surface area ( i.e., small fiber diameter) of the blend fibers from mucilage and poly(vinyl alcohol) allowed proper adhering and proliferation of the L929 fibroblasts cells [92]. In another study, randomly oriented poly(vinyl alcohol) fibers with average diameters ranged from 70 nm to 1120 nm affects cell responses [93].…”

Section: Electrospun Fiber Scaffolds Mediated Stem Cell Therapymentioning

confidence: 99%

“…Polysaccharides have an excellent biocompatibility and reasonable biodegradation rates while they also possess a relatively low cost. Plant mucilage is also a good material for use as a biomaterial scaffold as it promotes cell growth as well as having antioxidant, anti-inflammatory, and antibacterial properties [92]. Spirulina, on the other hand, is a material best used in combination with another polymer, preferably one that is biocompatible and biodegradable.…”

Section: Electrospun Fiber Scaffolds Mediated Stem Cell Therapymentioning

confidence: 99%

“…Lastly, a very popular and widely used biocompatible polymer is poly(vinyl alcohol) (PVA), and it is particularly useful as an additive or used in a blended fiber. In a study, PVA was used as an aiding agent in order to promote biocompatibility for cell growth in fiber scaffolds made from mucilage [92]. The results of the study showed that the addition of PVA promoted the formation of fibers in electrospinning and improved biocompatibility.…”

Section: Electrospun Fiber Scaffolds Mediated Stem Cell Therapymentioning

confidence: 99%

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The Role of Electrospun Fiber Scaffolds in Stem Cell Therapy for Skin Tissue Regeneration

2019

Med One

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Stem cell therapy has emerged as one of the topics in tissue engineering where undifferentiated and multipotent cells are strategically placed/ injected in tissue structure for cell regeneration. Over the years, stem cells have shown promising results in skin repairs for non-healing and/or chronic wounds. The addition of the stem cells around the wound site promotes signaling pathways for growth factors that regulate tissue reconstruction. However, injecting stem cells around the wound site has its drawbacks, including cell death due to lack of microenvironment cues. This particular issue is resolved when biomaterial scaffolds are involved in the cultivation and mechanical support of the stem cells. In this review, we describe the current models of stem cell therapy by injections and those that are done through cell cultures using electrospun fiber scaffolds. Electrospun fibers are considered as an ideal candidate for cell cultures due to their surface properties. Through the control of fiber morphology and fiber structure, cells are able to proliferate and differentiate into keratinocytes for skin tissue regeneration. Furthermore, we provide another perspective of using electrospun fibers and stem cells in a layer-by-layer structure for skin substitutes (dressing). Finally, electrospun fibers have the potential to incorporate bioactive agents to achieve controlled release properties, which is beneficial to the survival of the delivered stem cells or the recruitment of the cells. Overall, our work illustrates that electrospun fibers are ideal for stem cell cultures while serving as cell carriers for wound dressing materials.

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Section: Electrospun Fiber Scaffolds Mediated Stem Cell Therapymentioning

confidence: 99%

Section: Electrospun Fiber Scaffolds Mediated Stem Cell Therapymentioning

confidence: 99%

Section: Electrospun Fiber Scaffolds Mediated Stem Cell Therapymentioning

confidence: 99%

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The Role of Electrospun Fiber Scaffolds in Stem Cell Therapy for Skin Tissue Regeneration

2019

Med One

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“…More recently, agave bagasse is being explored for the production of cellulose nanofibers and nanocrystals [7,8,20,21] to further diversify the utilization of this biowaste. On the other hand, electro-spinning of biopolymers has become popular in the manufacture of nanofibers for a variety of added value applications, such as tissue engineering, drug release, sensors, and air and liquid filtration systems [22][23][24][25][26].Our main interest in electro-spinning agave cellulose nanofibers was to produce membranes that can retain suspended particles in water (due to the diameter of the fibers), a membrane with high porosity and good surface area properties. However, the main challenge with the preparation of electro-spun agave cellulose was the poor solubility of cellulose that was to be dispersed into an electro-spinnable liquid [24].…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Nanocellulose and Polycaprolactone Nanospun Composite Membranes and Their Potential for the Removal of Pollutants from Water

et al. 2020

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A composite membrane based on polycaprolactone (PCL) and cellulose nanofibers (CNF) with different compositions was prepared using the electro-spinning method, with the objective of developing organic membranes with good mechanical properties to remove contaminants from water. Water is a resource of primary importance for life and human activities. In this sense, cellulose obtained from agave bagasse and polycaprolactone nanofibers was used to prepare membranes that were tested by filtering tap water. The membranes obtained presented a porosity and structure on a nanometric scale. The water quality variables evaluated after filtration with the PCL/CNF membranes showed 100% turbidity removal, 100% conductivity, and heavy metal removal of the order of 75% to 99% for iron and chromium. CNF comprises biowaste derived from tequila production, and it has added value. Electro-spun CNF and PCL membranes can be applied as a "green" and eco-friendly filtration system for water purification.Molecules 2020, 25, 683 2 of 13 about 40% (wet basis) of over 1000 tons of agave grown in Mexico [11]. The availability of such large quantities of agave bagasse [9] poses serious disposal problems, since most of the biomass ends up in clandestine dumps (due to little environmental regulation), causing adverse effects on the fertility of farmland [12], contamination by leachates, and phytosanitary risks due to the inadequate incorporation of this material into the soil [13]. Chemically, agave bagasse is 44.5% cellulose, 25.3% hemicellulose, and 20.1% lignin [7]. Taking advantage of these constituents, there have been many efforts to use agave bagasse for various applications, e.g., the production of biopolymers [14,15], composting [16], animal nutrition [17], generation of biofuels [10,18], reinforcement materials [19], etc. More recently, agave bagasse is being explored for the production of cellulose nanofibers and nanocrystals [7,8,20,21] to further diversify the utilization of this biowaste. On the other hand, electro-spinning of biopolymers has become popular in the manufacture of nanofibers for a variety of added value applications, such as tissue engineering, drug release, sensors, and air and liquid filtration systems [22][23][24][25][26].Our main interest in electro-spinning agave cellulose nanofibers was to produce membranes that can retain suspended particles in water (due to the diameter of the fibers), a membrane with high porosity and good surface area properties. However, the main challenge with the preparation of electro-spun agave cellulose was the poor solubility of cellulose that was to be dispersed into an electro-spinnable liquid [24]. We overcame the poor solubility challenge by dissolving agave cellulose in dimethylformamide (DMF) and blending with polycaprolactone to prepare a composite electro-spun blend, subsequently producing electro-spun nanocellulose and polycaprolactone membranes. The main objective of using a copolymer blend was to improve technical properties, such as contaminant retention capacity, therma...

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Cranberry proanthocyanidins composite electrospun nanofibers as a potential alternative for bacterial entrapment applications

et al. 2022

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The interaction between A‐type interflavan bonds from cranberry proanthocyanidins (PAC) and surface virulence factors of extra‐intestinal pathogenic Escherichia coli (ExPEC) was studied. Electrospun nanofibers (ESNF) were fabricated using PAC and polycaprolactone (PCL) solutions and their physical and chemical properties were characterized. The ability of PAC:PCL composite ESNF to interact with and entrap ExPEC strain 5011 (ExPEC‐5011) was evaluated in vitro by plate culturing and when formulated as a biofilter and nanocoating. As a biofilter, the PAC:PCL ESNF exhibited a dose‐dependent ability to entrap ExPEC‐5011. Images from scanning electron and fluorescent microscopies revealed that ESNF sections with higher amounts of PAC led to higher bacterial entrapment. The effectiveness PAC:PCL ESNF to bind ExPEC when applied as a nanocoating was studied using ESNF‐coated polyvinyl chloride intermittent catheter. Results indicate that ExPEC‐5011 was entrapped well into the PAC:PCL ESNF coating on the catheter. Overall, our results suggest that incorporating the biomolecule PAC in ESNF is a potential means for applications requiring bacterial entrapment, such as biofunctionalization, biofiltration, and surface coating, among others.

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Electrospun plant mucilage nanofibers as biocompatible scaffolds for cell proliferation

Cited by 31 publications

References 26 publications

The Role of Electrospun Fiber Scaffolds in Stem Cell Therapy for Skin Tissue Regeneration

The Role of Electrospun Fiber Scaffolds in Stem Cell Therapy for Skin Tissue Regeneration

Nanocellulose and Polycaprolactone Nanospun Composite Membranes and Their Potential for the Removal of Pollutants from Water

Cranberry proanthocyanidins composite electrospun nanofibers as a potential alternative for bacterial entrapment applications

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