Electrospun nerve guide scaffold of poly(ε‐caprolactone)/collagen/nanobioglass: an in vitro study in peripheral nerve tissue engineering

Abstract

Among various methods, nerve tissue engineering (NTE) is one of the applicable methods to reconstruct damaged nerve tissues. Electrospinning technique and biomaterials are often considered to fabricate fibrous tissue engineered conduits which have great similarity to the extracellular matrix on fiber structure. Polymer blending is one of the most effective methods for the production of new materials with outstanding features. In this study, conduit structures as main part of the peripheral nerve regeneration based on polymer blend nanocomposites poly(ε-caprolactone)/collagen/nanobioglass (PCL/collagen/NBG) were manufactured by electrospinning technique. Various properties of electrospun mats were investigated by using contact angle, tensile, degradation time, porosity, scanning electron microscopy (SEM), Fourier-transform infrared (FTIR), and wide-angle X-ray scattering (WAXS). The SEM analysis was shown that size range and average pore size of polymer blend nanocomposite nanofibers were about 250-400 nm and 0.7 µm, respectively, with an optimum porosity of 62.5%. The XRD result was shown that synthesized nanoparticles of NBG had amorphous structures. Also, FTIR analysis indicated that good interaction between polymer-polymer macromolecules and polymer particles. The contact angle and tensile tests were indicated that electrospun webs showed good hydrophilicity and toughness properties. According to SEM, MTT assay and DAPI staining technique, the ability to support cell attachment and viability of samples were characterized. In vitro study indicated electrospun collagen/PCL/NBG nanofibrous conduit promoted Human Endometrial Stem cells (hEnSCs) adhesion and proliferation. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1960-1972, 2017.

Design and fabrication of poly (glycerol sebacate)‐based fibers for neural tissue engineering: Synthesis, electrospinning, and characterization

“…The crosslinked scaffolds were cut into the square samples (10 × 10 mm 2 ) and then immersed in 5‐mL phosphate‐buffered saline solution (PBS; pH = 7.4) and incubated at 37°C for 2 months . Following by each degradation time, each scaffold was washed with distilled water and dried in a vacuum oven for 24 hours.…”

“…Peripheral nerve injury resulted from trauma, tumor resection, and reconstructive surgery is a common problem that might lead to morbidity and impairment in affected people . Although several approaches have been so far reported to repair peripheral nerve gaps, there is no definitive solution for repairing long gaps .…”

“…Beside many materials employed in neural tissue engineering such as poly(ε‐caprolactone) (PCL), poly(L‐lactic acid) (PLLA), collagen, poly(3‐hydroxybutyrate) (PHB), and gelatin, poly (glycerol sebacate) (PGS) is a thermoset biodegradable elastomer that has received a great attention in design and development of neural tissue engineering in recent years. This could be attributed to its tunable physical characteristics and biodegradable rates .…”

Control of cell growth on 3D‐printed cell culture platforms for tissue engineering

“…Engineering tissues are built over scaffolds, which can influence aspects such as cell adhesion, proliferation rate, morphology, and differentiation. 1 For example, the skin is the main protective physical barrier of the human body, with the main function of protecting the underlying organs and body tissues from physical, mechanical, and chemical damage and from pathogenic microbial invasion. In addition, it plays an important role in the repairs after trauma through hemostasis, deposition of connective tissues, and wound contraction among other functions.…”

Use new poly (ε-caprolactone/collagen/NBG) nerve conduits along with NGF for promoting peripheral (sciatic) nerve regeneration in a rat

“…Although, this method is in most cases ineffective because of the mismatch of sizes between the donor and recipient tissues, donor site morbidity, loss of function at the donor sites and complex surgical procedures [2]. Neural tissue engineering is one of the most promising ways of treating nerve damage [3]. Therefore, To seek alternatives for autografts, the development of artificial nerve guidance conduits (NGCs) is ideal as an alternative [4,5].…”