Electrospun nanofibers for extraction of thymoquinone from Nigella-Stevia prior to detection using electrochemical biosensor based on GCE/rGO/CuO

Nejabati, Fatemeh; Ebrahimzadeh, Homeira

doi:10.1016/j.microc.2023.108545

Cited by 6 publications

(2 citation statements)

References 48 publications

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“…However, the reported yield from this process was low [31]. Recently, the use of electrospun nanofibers as a sorbent for TQ extraction has been proposed by Nejabati et al [32].…”

Section: Characterization Pharmacognostic Isolation and Purification ...mentioning

confidence: 99%

Thymoquinone: A Promising Therapeutic Agent for the Treatment of Colorectal Cancer

Kurowska,

Madej,

Strzalka-Mrozik

2023

CIMB

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Colorectal cancer (CRC) is one of the most commonly diagnosed cancers and is responsible for approximately one million deaths each year. The current standard of care is surgical resection of the lesion and chemotherapy with 5-fluorouracil (5-FU). However, of concern is the increasing incidence in an increasingly younger patient population and the ability of CRC cells to develop resistance to 5-FU. In this review, we discuss the effects of thymoquinone (TQ), one of the main bioactive components of Nigella sativa seeds, on CRC, with a particular focus on the use of TQ in combination therapy with other chemotherapeutic agents. TQ exhibits anti-CRC activity by inducing a proapoptotic effect and inhibiting proliferation, primarily through its effect on the regulation of signaling pathways crucial for tumor progression and oxidative stress. TQ can be used synergistically with chemotherapeutic agents to enhance their anticancer effects and to influence the expression of signaling pathways and other genes important in cancer development. These data appear to be most relevant for co-treatment with 5-FU. We believe that TQ is a suitable candidate for consideration in the chemoprevention and adjuvant therapy for CRC, but further studies, including clinical trials, are needed to confirm its safety and efficacy in the treatment of cancer.

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“…However, the reported yield from this process was low [31]. Recently, the use of electrospun nanofibers as a sorbent for TQ extraction has been proposed by Nejabati et al [32].…”

Section: Characterization Pharmacognostic Isolation and Purification ...mentioning

confidence: 99%

Thymoquinone: A Promising Therapeutic Agent for the Treatment of Colorectal Cancer

Kurowska,

Madej,

Strzalka-Mrozik

2023

CIMB

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“…Electrospinning is one of the most widely used techniques to prepare flexible polymeric fibers, , the main advantage being the ability to control the physical and chemical properties of the fibers as well as their dimensions, which can reach the nanometer scale under the proper conditions . However, the conductivity of the polymeric scaffold represents a challenge, which can be overcome by direct metallization of the fiber surface with transition/noble metals. − Several metals have been applied to functionalize electrospun fibers in different nanoscaled morphologies. , Palladium represents an alternative for biosensing applications, particularly for hydrogen peroxide reduction, which occurs close to 0.0 V vs Ag/AgCl, thus with reduced interferences. − In this context, several palladium nanoscaled materials have been used to develop electrochemical biosensors, , however, most of them lack flexibility, which is critical for wearable sensing devices.…”

Section: Introductionmentioning

confidence: 99%

Palladium-Coated Submicron Electrospun Polymeric Fibers with Immobilized Uricase for Uric Acid Determination in Body Fluids

Leote,

Crisan,

Matei

et al. 2024

ACS Appl. Polym. Mater.

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This work describes the development of a flexible uric acid (UA) biosensor based on palladium-coated submicrometer electrospun poly(methylmethacrylate) (PMMA) fibers metalized with gold and attached to polyethylene terephthalate substrate (Pd/Au/PMMA/PET). The morphological characterization conducted by scanning electron microscopy revealed nanoscale Pd dendritic structures. Electrochemical investigations in the absence and in the presence of redox probes demonstrated that these Pd nanostructures are responsible for a six-fold increase in the electroactive area and enhanced electron transfer kinetics when compared to the gold-coated electrospun fibers. The UA biosensor obtained by immobilizing the uricase enzyme (UrOx) onto the Pd/Au/PMMA/PET electrode surface, allowed UA detection with a sensitivity of 431 μA cm −2 mM −1 and a limit of detection of 12 μM. Investigation of the redox reactions of hydrogen peroxide (a product of the enzymatic oxidation of UA by UrOx) at the Pd/Au/PMMA/PET electrode demonstrated that the working principle of the biosensor is based on the reduction of PdO produced at the electrode surface during the spontaneous reduction of hydrogen peroxide on Pd. This allows a biosensor operating potential of −0.05 V (vs Ag/AgCl) with high selectivity. The UrOx/Pd/Au/PMMA/PET biosensor was applied for UA detection in body fluids (sweat, urine, and blood serum) with recovery values between 98 and 105%, which were validated by high-performance liquid chromatography analysis. The stability of the device was evaluated over a period of 3 months, retaining 78% of the initial sensitivity, and reproducibility with RSD = 4.9% was achieved. The analytical performance of the biosensor under harsh mechanical deformations and at physiological temperatures demonstrated the potential applications of the device to wearable sensing platforms.

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A novel sorbent based on electrospun for electrically-assisted solid phase microextraction of six non-steroid anti-inflammatory drugs, followed by quantitation with HPLC-UV in human plasma samples

Nejabati,

Ebrahimzadeh

2024

Analytica Chimica Acta

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Electrospun nanofibers for extraction of thymoquinone from Nigella-Stevia prior to detection using electrochemical biosensor based on GCE/rGO/CuO

Cited by 6 publications

References 48 publications

Thymoquinone: A Promising Therapeutic Agent for the Treatment of Colorectal Cancer

Thymoquinone: A Promising Therapeutic Agent for the Treatment of Colorectal Cancer

Palladium-Coated Submicron Electrospun Polymeric Fibers with Immobilized Uricase for Uric Acid Determination in Body Fluids

A novel sorbent based on electrospun for electrically-assisted solid phase microextraction of six non-steroid anti-inflammatory drugs, followed by quantitation with HPLC-UV in human plasma samples

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