Electrospun nanofibers as versatile interfaces for efficient gene delivery

Lee, Slgirim; Jin, Gyuhyung; Jang, Jae Won

doi:10.1186/1754-1611-8-30

Cited by 58 publications

(52 citation statements)

References 116 publications

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“…Direct adsorption relies on non-covalent chemical interactions, such as electrostatic and van der Waals interactions, between the drug and the polymer to facilitate binding of the drug to the scaffold. Drug diffusion from the polymer surface is facilitated due to the high porosity and surface area offered by the use of nanofibers, often times leading to a high initial release of drug upon contact with release medium, referred to as “burst release.” This method is particularly desirable for drugs and gene constructs that may be sensitive to the organic solvents or high voltage used in electrospinning [16]. …”

Section: Introductionmentioning

confidence: 99%

“…Another less common method of drug loading includes immobilization of drug onto the polymer surface through methods such as layer-by-layer deposition. This strategy is particularly useful for charged entities such as negatively changed DNA for gene delivery applications, where a positively charged macromolecule such as polyethylenimine can be used as a countercharge [16, 26]. …”

Section: Introductionmentioning

confidence: 99%

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Poly(lactic acid) nanofibrous scaffolds for tissue engineering

Santoro

Shah

Walker

et al. 2016

Advanced Drug Delivery Reviews

358

182

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Poly(lactic acid) (PLA) is a synthetic polyester that has shown extensive utility in tissue engineering. Synthesized either by ring opening polymerization or polycondensation, PLA hydrolytically degrades into lactic acid, a metabolic byproduct, making it suitable for medical applications. Specifically, PLA nanofibers have widened the possible uses of PLA scaffolds for regenerative medicine and drug delivery applications. The use of nanofibrous scaffolds imparts a host of desirable properties, including high surface area, biomimicry of native extracellular matrix architecture, and tuning of mechanical properties, all of which are important facets of designing scaffolds for a particular organ system. Additionally, nanofibrous PLA scaffolds hold great promise as drug delivery carriers, where fabrication parameters and drug-PLA compatibility greatly affect the drug release kinetics. In this review, we present the latest advances in the use of PLA nanofibrous scaffolds for musculoskeletal, nervous, cardiovascular, and cutaneous tissue engineering and offer perspectives on their future use.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Poly(lactic acid) nanofibrous scaffolds for tissue engineering

Santoro

Shah

Walker

et al. 2016

Advanced Drug Delivery Reviews

358

182

View full text Add to dashboard Cite

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“…A variety of polymeric matrices have been employed to provide sustained and localized viral vector delivery including from formats such as microparticles [32] and electrospun scaffolds [33,34]. In terms of electrospun materials, the generation of coaxial fibers for the delivery of bioactive agents has been used to overcome some of the limitations associated with conventional single stream electrospinning [35,36]. The inclusion of bioactive agents in hydrophilic cores encapsulated by a hydrophobic sheath can protect the agents from direct exposure to harsh organic solvents necessary for structural polymer dissolution.…”

Section: Discussionmentioning

confidence: 99%

Sustained viral gene delivery from a micro-fibrous, elastomeric cardiac patch to the ischemic rat heart

Matsumura

Tang

et al. 2017

Biomaterials

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Biodegradable and elastomeric patches have been applied to the surface of infarcted hearts as temporary mechanical supports to effectively alter adverse left ventricular remodeling processes. In this report, recombinant adeno-associated virus (AAV), known for its persistent transgene expression and low pathogenicity, was incorporated into elastomeric polyester urethane urea (PEUU) and polyester ether urethane urea (PEEUU) and processed by electrospinning into two formats (solid fibers and core-sheath fibers) designed to influence the controlled release behavior. The extended release of AAV encoding green fluorescent protein (GFP) was assessed in vitro. Sustained and localized viral particle delivery was achieved over 2 months in vitro. The biodegradable cardiac patches with or without AAV-GFP were implanted over rat left ventricular lesions three days following myocardial infarction to evaluate the transduction effect of released viral vectors. AAV particles were directly injected into the infarcted hearts as a control. Cardiac function and remodeling were significantly improved for 12 weeks after patch implantation compared to AAV injection. More GFP genes was expressed in the AAV patch group than AAV injection group, with both α-SMA positive cells and cardiac troponin T positive cells transduced in the patch group. Overall, the extended release behavior, prolonged transgene expression, and elastomeric mechanical properties make the AAV-loaded scaffold an attractive option for cardiac tissue engineering where both gene delivery and appropriate mechanical support are desired.

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“…The second is the direct injection of genes to disease target tissues . Third is the controlled release of genes from biomaterials such as hydrogels, polyester scaffolds, or metallic stents either by encapsulation or surface immobilization of gene‐containing vectors. In particular, surface immobilization of gene vectors has been studied to achieve both an efficient transfection rate and controlling of immune responses .…”

Section: Introductionmentioning

confidence: 99%

Functional Polysaccharide Sutures Prepared by Wet Fusion of Interfacial Polyelectrolyte Complexation Fibers

Park

et al. 2017

Adv Funct Materials

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This study reports polysaccharide-based fibers that can be utilized as biocompatible functional sutures. Fibers are spontaneously formed by spinning at the interface between two oppositely charged polysaccharide solutions. Unlike the common belief that polysaccharide fibers prepared by electrostatic interactions would exhibit weak mechanical strength, it is demonstrated that fibers spun at the interface between two droplets of positively charged chitosan and negatively charged heparin can exhibit high mechanical strength through spontaneous wet-state fusion of interfiber strands at a spinning wheel. Dry solidification results in multistranded fibers that were ≈100 µm in diameter with a tensile strength of ≈220 MPa. Post fibrous manipulation yields various morphology with straight or twisted fibers, fabrics, or springs. To demonstrate application of the fiber, it is applied as a medical suture. As heparin has a unique ability to bind adeno-associated virus (AAV), a therapeutic, biocompatible suture exhibiting localized AAV-mediated gene delivery function can be prepared. This study shows that multistrand fusion of fibers, formed by weak, electrostatic interactions and followed by drying solidification counterintuitively results in mechanically strong, functional fibers with various potential applications.

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Electrospun nanofibers as versatile interfaces for efficient gene delivery

Cited by 58 publications

References 116 publications

Poly(lactic acid) nanofibrous scaffolds for tissue engineering

Poly(lactic acid) nanofibrous scaffolds for tissue engineering

Sustained viral gene delivery from a micro-fibrous, elastomeric cardiac patch to the ischemic rat heart

Functional Polysaccharide Sutures Prepared by Wet Fusion of Interfacial Polyelectrolyte Complexation Fibers

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