Electrospun nano-fibre mats with antibacterial properties from quaternised chitosan and poly(vinyl alcohol)

Ignatova, Miléna; Starbova, K.; Маркова, Н.; Manolova, Nevena; Rashkov, Iliya

doi:10.1016/j.carres.2006.05.006

Cited by 322 publications

(193 citation statements)

References 28 publications

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“…Also, the nonvolatile nature of the cross-linking agent prevents the use of the vapor-phase crosslinking method (29)(30)(31). Several studies have described various other methods of creating cross-linked fibers; such as using heat (32)(33)(34)(35)(36) or UV radiation (20,(37)(38)(39) to initiate the cross-linking reaction during or after the synthesis of the fibers. Heat and UV light, however, have known microbicidal properties and thus are not suitable for use in this study.…”

Section: Resultsmentioning

confidence: 99%

Engineering of bio-hybrid materials by electrospinning polymer-microbe fibers

Liu

Rafailovich

Malal

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Although microbes have been used in industrial and niche applications for several decades, successful immobilization of microbes while maintaining their usefulness for any desired application has been elusive. Such a functionally bioactive system has distinct advantages over conventional batch and continuous-flow microbial reactor systems that are used in various biotechnological processes. This article describes the use of polyethylene oxide 99-polypropylene oxide 67-polyethylene oxide99 triblock polymer fibers, created via electrospinning, to encapsulate microbes of 3 industrially relevant genera, namely, Pseudomonas, Zymomonas, and Escherichia. The presence of bacteria inside the fibers was confirmed by fluorescence microscopy and SEM. Although the electrospinning process typically uses harsh organic solvents and extreme conditions that generally are harmful to bacteria, we describe techniques that overcome these limitations. The encapsulated microbes were viable for several months, and their metabolic activity was not affected by immobilization; thus they could be used in various applications. Furthermore, we have engineered a microbe-encapsulated cross-linked fibrous polymeric material that is insoluble. Also, the microbe-encapsulated active matrix permits efficient exchange of nutrients and metabolic products between the microorganism and the environment. The present results demonstrate the potential of the electrospinning technique for the encapsulation and immobilization of bacteria in the form of a synthetic biofilm, while retaining their metabolic activity. This study has wide-ranging implications in the engineering and use of novel bio-hybrid materials or biological thin-film catalysts.biofilm ͉ cross-linking ͉ immobilization ͉ microbial ͉ Zymomonas

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Section: Resultsmentioning

confidence: 99%

Engineering of bio-hybrid materials by electrospinning polymer-microbe fibers

Liu

Rafailovich

Malal

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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“…But a recent study by Ignatova [20] has been carried out using Chitosan to create electrospun nano-fiber mats that have antibacterial activity. The experiment involves creating fibers in the range of 60-200nm out of quaternized Chitosan (QCh) mixed with poly (vinyl alcohol) (PVA) and were stabilized successfully against dissolution in the aqueous environment using photomediated cross-linking.…”

Section: Chitosan Nanofibermentioning

confidence: 99%

“…The results highlight the potential of electrospun mats to be used for wound-dressings as they are able to reduce the threat of secondary infection by bacteria. Additional chitosan also provides all the desirable properties that are required for fast wound healing that is non-toxic, has hemostatic activity, biodegradability and the ability to affect macrophage function [20].…”

Section: Chitosan Nanofibermentioning

confidence: 99%

“…Yacoby [21] has devised an efficient targeted drug platform to address the requirements of targeted drug delivery, in that it is able to selectively bind to its target, provide large drug delivery payload and timely drug release. Their approach to such criteria was in the development of a drug-carrying phage nanoparticle that was successfully able to inhibit the growth of both gram-negative and gram-positive bacteria [20]. A phage was used as the drug-carrier as it has nanometric parameters that offer a unique and excellent drug-carrying ability, when compared to other particle drug-carrying devices that are commonly used such as liposomes or virus-like particles [19,20].…”

Section: Targeted Drug-carrying Phage Medicinesmentioning

confidence: 99%

“…Their approach to such criteria was in the development of a drug-carrying phage nanoparticle that was successfully able to inhibit the growth of both gram-negative and gram-positive bacteria [20]. A phage was used as the drug-carrier as it has nanometric parameters that offer a unique and excellent drug-carrying ability, when compared to other particle drug-carrying devices that are commonly used such as liposomes or virus-like particles [19,20]. This feature was also enhanced by use of clever chemistry through the introduction of amino sugar-based aminoglycosides as branched, hydrophilic linkers.…”

Section: Targeted Drug-carrying Phage Medicinesmentioning

confidence: 99%

See 2 more Smart Citations

Applications of Nanomedicine in Antibacterial Medical Therapeutics and Diagnostics~!2009-08-26~!2009-11-25~!2010-02-24~!

Matthews¹,

Kanwar²,

Zhou³

et al. 2010

TOTMJ

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Abstract:The need for new and effective/efficient antibacterial therapeutics and diagnostics is necessary if we want to be able to maintain and improve the protection against pathogenic bacteria. Bacteria are becoming increasingly resistant to traditionally used antibiotics and as a result are a major health concern. The number of deaths and hospitalizations due to bacteria is increasing. Current methods of bacterial diagnostics are inefficient as they lack speed and ultra sensitivity and cannot be performed on site. This is where nanomedicine is playing a vital role. The discovery of new and innovative materials through the improvement in fabrication techniques has seen the establishment of an influx of novel antibacterial therapeutics and diagnostics. The goal of this review is to highlight the research that has been done through the implementation of nanomaterials and nanotechnologies for antibacterial medical therapeutic and diagnostic.

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