Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Fu, Wei; Liu, Zhenling; Feng, Bei; Hu, Renjie; He, Xiaomin; Wang, Hao; Yin, Meng; Huang, Huimin; Zhang, Haibo; Wang, Wei

doi:10.2147/ijn.s61375

Cited by 208 publications

(125 citation statements)

References 38 publications

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“…In fact, 10 s after the water drop (i.e. time set to allow the drop to accommodate and interact with the surface [44]), the membranes containing low (5 wt.%) and medium (15 wt.%) ZnO contents could not maintain a convex shape of the water drops, thus precluding the contact angle measurement. This, perhaps, demonstrates that the latter membranes were very hydrophilic, and therefore, they can be considered promising for GTR/GBR applications.…”

Section: Discussionmentioning

confidence: 99%

Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration

et al. 2015

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Objectives This study reports on the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel ZnO-loaded membranes for guided tissue/bone regeneration (GTR/GBR). Methods Poly(ε-caprolactone) (PCL) and PCL/gelatin (PCL/GEL) were dissolved in hexafluoropropanol and loaded with ZnO at distinct concentrations: 0 (control), 5, 15, and 30 wt.%. Electrospinning was performed using optimized parameters and the fibres were characterized via scanning and transmission electron microscopies (SEM/TEM), energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), contact angle (CA), mechanical testing, antimicrobial activity against periodontopathogens, and cytotoxicity test using human dental pulp stem cells (hDPSCs). Data were analyzed using ANOVA and Tukey (α=5%). Results ZnO nanoparticles were successfully incorporated into the overall submicron fibres, which showed fairly good morphology and microstructure. Upon ZnO nanoparticles’ incorporation, the PCL and PCL/GEL fibres became thicker and thinner, respectively. All GEL-containing membranes showed lower CA than the PCL-based membranes, which were highly hydrophobic. Overall, the mechanical properties of the membranes were reduced upon ZnO incorporation, except for PCL-based membranes containing ZnO at the 30 wt.% concentration. The presence of GEL enhanced the stretching ability of membranes under wet conditions. All ZnO-containing membranes displayed antibacterial activity against the bacteria tested, which was generally more pronounced with increased ZnO content. All membranes synthesized in this study demonstrated satisfactory cytocompatibility, although the presence of 30 wt.% ZnO led to decreased viability. Significance Collectively, this study suggests that PCL- and PCL/GEL-based membranes containing a low content of ZnO nanoparticles can potentially function as a biologically safe antimicrobial GTR/GBR membrane.

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Section: Discussionmentioning

confidence: 99%

Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration

et al. 2015

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“…Numerous methods now exist to electrospin nanofibers, including traditional needle arrays and needle-free techniques [25, 26] developed to improve fiber formation and production rates: these include bubble electrospinning [180] and microfluidic electrospinning [181]. Synthetic polymers and biological proteins are electrospun using these techniques independently [182, 183] or in combination [184, 185]. The diversity of electrospinning techniques and electrospun materials has translated to numerous fibrous tissue engineering applications including ligament, tendon, skeletal muscle, skin, blood vessel, and neural scaffolding [186].…”

Section: Fibrous Scaffold Production Techniquesmentioning

confidence: 99%

Fibrous scaffolds for building hearts and heart parts

Capulli

MacQueen

Sheehy

et al. 2016

Advanced Drug Delivery Reviews

110

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Extracellular matrix (ECM) structure and biochemistry provide cell-instructive cues that promote and regulate tissue growth, function, and repair. From a structural perspective, the ECM is a scaffold that guides the self-assembly of cells into distinct functional tissues. The ECM promotes the interaction between individual cells and between different cell types, and increases the strength and resilience of the tissue in mechanically dynamic environments. From a biochemical perspective, factors regulating cell-ECM adhesion have been described and diverse aspects of cell-ECM interactions in health and disease continue to be clarified. Natural ECMs therefore provide excellent design rules for tissue engineering scaffolds. The design of regenerative three-dimensional (3D) engineered scaffolds is informed by the target ECM structure, chemistry, and mechanics, to encourage cell infiltration and tissue genesis. This can be achieved using nanofibrous scaffolds composed of polymers that simultaneously recapitulate 3D ECM architecture, high-fidelity nanoscale topography, and bio-activity. Their high porosity, structural anisotropy, and bio-activity present unique advantages for engineering 3D anisotropic tissues. Here, we use the heart as a case study and examine the potential of ECM-inspired nanofibrous scaffolds for cardiac tissue engineering. We asked: Do we know enough to build a heart? To answer this question, we tabulated structural and functional properties of myocardial and valvular tissues for use as design criteria, reviewed nanofiber manufacturing platforms and assessed their capabilities to produce scaffolds that meet our design criteria. Our knowledge of the anatomy and physiology of the heart, as well as our ability to create synthetic ECM scaffolds have advanced to the point that valve replacement with nanofibrous scaffolds may be achieved in the short term, while myocardial repair requires further study in vitro and in vivo.

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“…19–21 In spite of the high mechanical stability of these polymers, they lack the innate reactive sites for cell adhesion. The hydrophobic nature of PCL, PU, and PLA also tends to attract platelet and plasma protein adhesion, leading to the aggregation and intimal hyperplasia of the artificial blood vessels.…”

Section: Introductionmentioning

confidence: 99%

Highly Compliant Vascular Grafts with Gelatin-Sheathed Coaxially Structured Nanofibers

et al. 2015

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We have developed three types of materials composed of polyurethane–gelatin, polycaprolactone–gelatin, or polylactic acid–gelatin nanofibers by coaxially electro-spinning the hydrophobic core and gelatin sheath with a ratio of 1:5 at fixed concentrations. Results from attenuated total reflection-Fourier transformed infrared spectroscopy demonstrated the gelatin coating around nanofibers in all of the materials. Transmission electron microscopy images further displayed the core–sheath structures showing the core-to-sheath thickness ratio varied greatly with the highest ratio found in polyurethane-gelatin nanofibers. Scanning electron microscopy images revealed similar, uniform fibrous structures in all of the materials, which changed with genipin cross-linking due to interfiber interactions. Thermal analyses revealed varied interactions between the hydrophilic sheath and hydrophobic core among the three materials, which likely caused different core–sheath structures, and thus physicomechanical properties. The addition of gelatin around the hydrophobic polymer and their interactions led to the formation of graft scaffolds with tissue-like viscoelasticity, high compliance, excellent swelling capability, and absence of water permeability while maintaining competent tensile modulus, burst pressure, and suture retention. The hydrogel-like characteristics are advantageous for vascular grafting use, because of the capability of bypassing preclotting prior to implantation, retaining vascular fluid volume, and facilitating molecular transport across the graft wall, as shown by coculturing vascular cells sandwiched over a thick-wall scaffold. Varied core–sheath interactions within scaffolding nanofibers led to differences in graft functional properties such as water swelling ratio, compliance, and supporting growth of cocultured vascular cells. The PCL–gelatin scaffold with thick gelatin-sheathed nanofibers demonstrated a more compliant structure, elastic mechanics, and high water swelling property. Our results demonstrate a feasible approach to produce new hybrid, biodegradable nanofibrous scaffold biomaterials with interactive core–sheath structure, good biocompatibility, and tissue-like viscoelasticity, which may reduce potential problems with the use of individual polymers for vascular grafts.

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Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Cited by 208 publications

References 38 publications

Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration

Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration

Fibrous scaffolds for building hearts and heart parts

Highly Compliant Vascular Grafts with Gelatin-Sheathed Coaxially Structured Nanofibers

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