Electrospun fine-textured scaffolds for heart tissue constructs

Zong, Xinhua; Bien, Harold; Chung, Chiung-Yin; Yin, Lihong; Fang, Dufei; Hsiao, Benjamin S.; Chu, Benjamin; Entcheva, Emilia

doi:10.1016/j.biomaterials.2005.01.052

Cited by 591 publications

(386 citation statements)

References 27 publications

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“…Such scaffolds hold the promise to provide the topographic cues to the seeded cells and may potentially enhance tissue regeneration. A few studies have shown that aligned electrospun scaffolds are able to provide contact guidance to cultured cells, resulting in an elongation and alignment of cells along the axes of the fibers [2][3][4]. The majority of the studies revolve around the evaluation of cell morphological changes, whilst some assess the preservation of cell phenotype via gene or protein expression analysis [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…The majority of the studies revolve around the evaluation of cell morphological changes, whilst some assess the preservation of cell phenotype via gene or protein expression analysis [5,6]. Quantitative evaluation of changes in cellular function under the influence of topographic cues provided by electrospun fibers is still limited [3,7,8].…”

Section: Introductionmentioning

confidence: 99%

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The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

et al. 2008

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Peripheral nerve regeneration can be enhanced by the stimulation of formation of bands of Büngner prior to implantation. Aligned electrospun poly(ε-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to human Schwann cells. After 7 days of culture, cell cytoskeleton and nuclei were observed to align and elongate along the fiber axes, emulating the structure of bands of Büngner. Microarray analysis revealed a general down-regulation in expression of neurotrophin and neurotrophic receptors in aligned cells as compared to cells seeded on twodimensional PCL film. Real-time-PCR analyses confirmed the up-regulation of early myelination marker, MAG, and the down-regulation of NCAM-1, a marker of immature Schwann cells. Similar gene expression changes were also observed on cells cultured on randomly-oriented PCL electrospun fibers. However, up-regulation of the myelin-specific gene, P0, was observed only on aligned electrospun fibers, suggesting the propensity of aligned fibers in promoting Schwann cell maturation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

et al. 2008

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“…In vitro evaluations underpinned the suitability of electrospun membranes for cardiomyocyte guidance and differentiation as well as maintenance of their contractile function [20][21][22]. The fibrous, anisotropic architecture of electrospun scaffolds compared to solid substrates proved superior for cardiomyocyte maturation and, in particular, cell infiltration and multi-layered tissue formation [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Plasma-functionalized electrospun matrix for biograft development and cardiac function stabilization

et al. 2014

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Cardiac tissue engineering approaches can deliver large numbers of cells to the damaged myocardium and have thus increasingly been considered as a possible curative treatment to counteract the high prevalence of progressive heart failure after myocardial infarction (MI). Optimal scaffold architecture and mechanical and chemical properties, as well as immune-and bio-compatibility, need to be addressed. We demonstrated that radio-frequency plasma surface functionalized electrospun poly (e-caprolactone) (PCL) fibres provide a suitable matrix for bone-marrow-derived mesenchymal stem cell (MSC) cardiac implantation. Using a rat model of chronic MI, we showed that MSC-seeded plasma-coated PCL grafts stabilized cardiac function and attenuated dilatation. Significant relative decreases of 13% of the ejection fraction (EF) and 15% of the fractional shortening (FS) were observed in sham treated animals; respective decreases of 20% and 25% were measured 4 weeks after acellular patch implantation, whereas a steadied function was observed 4 weeks after MSC-patch implantation (relative decreases of 6% for both EF and FS).

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“…In vitro studies of biodegradable non-woven PLGA-based scaffolds using primary CMs from rats have shown a dose-response effect of the PGA concentration on the degradation rate and the pH value changes [87]. It was found that CMs had a preference for relatively hydrophobic surfaces such as PLLA.…”

Section: Scaffolds Based On Plla Plga and Their Copolymersmentioning

confidence: 99%

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

et al. 2017

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Please cite this article as: Kitsara, M., Agbulut, O., Kontziampasis, D., Chen, Y., Menasché, P., Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering, Acta Biomaterialia (2016), doi: http://dx.doi.org/ 10. 1016/j.actbio.2016.11.014 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractCardiac cell therapy holds a real promise for improving heart function and especially of the chronically failing myocardium. Embedding cells into 3D biodegradable scaffolds may better preserve cell survival and enhance cell engraftment after transplantation, consequently improving cardiac cell therapy compared with direct intramyocardial injection of isolated cells.The primary objective of a scaffold used in tissue engineering is the recreation of the natural 3D environment most suitable for an adequate tissue growth. An important aspect of this commitment is to mimic the fibrillar structure of the extracellular matrix, which provides essential guidance for cell organization, survival, and function. Recent advances in nanotechnology have significantly improved our capacities to mimic the extracellular matrix. Among them, electrospinning is well known for being easy to process and cost effective. Consequently, it is becoming increasingly popular for biomedical applications and it is most definitely the cutting edge technique to make scaffolds that mimic the extracellular matrix for industrial applications.Here, the desirable physico-chemical properties of the electrospun scaffolds for cardiac therapy are described, and polymers are categorized to natural and synthetic. Moreover, the methods used for improving functionalities by providing cells with the necessary chemical cues and a more in vivo-like environment are reported.

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Electrospun fine-textured scaffolds for heart tissue constructs

Cited by 591 publications

References 27 publications

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

Plasma-functionalized electrospun matrix for biograft development and cardiac function stabilization

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

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