Electrospun Fibers as Substrates for Peripheral Nerve Regeneration

Mey, Jörg; Brook, Gary; Hodde, Dorothée; Kriebel, Andreas

doi:10.1007/12_2011_122

Cited by 12 publications

(18 citation statements)

References 171 publications

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“…Increasingly sophisticated bioengineering techniques are being developed to generate spatially defined 3D scaffolds that can mimic the molecular and physical characteristics of nervous tissue for studying cell–substrate interactions and for the design of scaffolds that are capable of promoting functional tissue repair (Dalton & Mey, ; Hodde et al ., ; Mey et al ., ). In the present investigation, we modified a protocol of Yang et al .…”

Section: Discussionmentioning

confidence: 97%

“…In recent years, bioengineering‐based strategies have developed from simple hollow conduits to those containing microengineered, longitudinally orientated guidance substrates. More recent designs contain guidance cues embedded in 3D hydrogels (Dalton & Mey, ; Hodde et al ., ; Mey et al ., ). The successful design of scaffolds that provide biochemical and topographical support for nerve repair requires an understanding of how the main cell types interact with the individual components of the conduit, and with the entire ensemble.…”

Section: Introductionmentioning

confidence: 97%

“…More recent designs contain guidance cues embedded in 3D hydrogels Hodde et al, 2012;Mey et al, 2012). The successful design of scaffolds that provide biochemical and topographical support for nerve repair requires an understanding of how the main cell types interact with the individual components of the conduit, and with the entire ensemble.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, electrospun nanofibres have generated great interest in bioengineering strategies for peripheral nerve repair (Corey et al, 2007;Schnell et al, 2007;Kim et al, 2008;Clements et al, 2009). Such nanofibres have the advantage of presenting high surface area-to-volume topographical cues for the support of cell adhesion, and directional process extension and migration Mey et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of cell–substrate interactions between Schwann cells and three‐dimensional fibrin hydrogels containing orientated nanofibre topographical cues

Hodde

Gerardo‐Nava

Wöhlk

et al. 2015

Eur J of Neuroscience

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The generation of complex three-dimensional bioengineered scaffolds that are capable of mimicking the molecular and topographical cues of the extracellular matrix found in native tissues is a field of expanding research. The systematic development of such scaffolds requires the characterisation of cell behaviour in response to the individual components of the scaffold. In the present investigation, we studied cell-substrate interactions between purified populations of Schwann cells and three-dimensional fibrin hydrogel scaffolds, in the presence or absence of multiple layers of highly orientated electrospun polycaprolactone nanofibres. Embedded Schwann cells remained viable within the fibrin hydrogel for up to 7 days (the longest time studied); however, cell behaviour in the hydrogel was somewhat different to that observed on the two-dimensional fibrin substrate: Schwann cells failed to proliferate in the fibrin hydrogel, whereas cell numbers increased steadily on the two-dimensional fibrin substrate. Schwann cells within the fibrin hydrogel developed complex process branching patterns, but, when presented with orientated nanofibres, showed a strong tendency to redistribute themselves onto the nanofibres, where they extended long processes that followed the longitudinal orientation of the nanofibres. The process length along nanofibre-containing fibrin hydrogel reached near-maximal levels (for the present experimental conditions) as early as 1 day after culturing. The ability of this three-dimensional, extracellular matrix-mimicking scaffold to support Schwann cell survival and provide topographical cues for rapid process extension suggest that it may be an appropriate device design for the bridging of experimental lesions of the peripheral nervous system.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterisation of cell–substrate interactions between Schwann cells and three‐dimensional fibrin hydrogels containing orientated nanofibre topographical cues

Hodde

Gerardo‐Nava

Wöhlk

et al. 2015

Eur J of Neuroscience

Self Cite

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“…For instance, oligopeptides consist of alternating hydrophilic and hydrophobic amino acids forming stable β-sheet structures. When an aqueous peptide solution is added to a physiological salt-containing solution, β-sheets pack together to form double-layered β-sheet nanofibres, without the need for temperature changes [51]. Self-assembly is a simple process for nanofibre fabrication.…”

Section: Fabricationmentioning

confidence: 99%

Biodegradable polymeric nanomaterials

Rohman¹,

Spadavecchia²

2016

Nanomaterials and Regenerative Medicine

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Design Strategies for Reduced‐scale Surface Composition Gradients via CVD Copolymerization

Elkasabi

Ross

et al. 2013

Chemical Vapor Deposition

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A new method for generating and modeling reduced-scale copolymer gradients by CVD is reported. By exploiting diffusion through confined channels, functionalized [2.2]paracyclophanes are copolymerized into their poly(p-xylylene) (PPX) analogues as a composition gradient. Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) are used to verify the gradient composition profiles. Gradients are deposited on both flat substrates and 3-dimensional cylinders. Both the thickness and compositional profiles are fitted to a diffusion-based model using realistic physical parameters. The derived equation can be generalized and optimized for any copolymerization gradient through a confined geometry, thus allowing for broad applicability to other copolymer systems.

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Electrospun Fibers as Substrates for Peripheral Nerve Regeneration

Cited by 12 publications

References 171 publications

Characterisation of cell–substrate interactions between Schwann cells and three‐dimensional fibrin hydrogels containing orientated nanofibre topographical cues

Characterisation of cell–substrate interactions between Schwann cells and three‐dimensional fibrin hydrogels containing orientated nanofibre topographical cues

Biodegradable polymeric nanomaterials

Design Strategies for Reduced‐scale Surface Composition Gradients via CVD Copolymerization

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