Electrospun elastin-like polypeptide enriched polyurethanes and their interactions with vascular smooth muscle cells

Blit, Patrick H.; Battiston, Kyle G.; Yang, Meilin; Santerre, J. Paul; Woodhouse, Kimberly A.

doi:10.1016/j.actbio.2012.03.032

Cited by 47 publications

(30 citation statements)

References 37 publications

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“…Also elastin‐derived peptides possess several biological activities as cell migration, differentiation, proliferation, chemotaxis, and upregulation of metalloproteinases (MMPs) and for this reason there are also known as “matrikines.” Literature reports the use of elastin‐like polypeptides, coated on synthetic polymers, for vascular applications. As expected, elastin surface modified materials demonstrated enhanced smooth muscle cells adhesion and exhibited a spindle‐shape morphology, actin filament organization, and smooth muscle myosin heavy chain (MHC) expression showing elastin‐like polypeptides as promising surface modifiers for scaffolds in the engineering of contractile vascular tissues . As a novelty with respect to the cited works, in our research the use of recombinant elastin‐like polypeptides, made by a short repetitive monomeric sequence (exons 23 and 24 of native tropoelastin), was not limited as surface coating but it is used as co‐polymer in a 3D matrix.…”

Section: Introductionmentioning

confidence: 59%

Human elastin polypeptides improve the biomechanical properties of three‐dimensional matrices through the regulation of elastogenesis

Boccafoschi

Ramella

Sibillano

et al. 2014

J Biomedical Materials Res

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The replacement of diseased tissues with biological substitutes with suitable biomechanical properties is one of the most important goal in tissue engineering. Collagen represents a satisfactory choice for scaffolds. Unfortunately, the lack of elasticity represents a restriction to a wide use of collagen for several applications. In this work, we studied the effect of human elastin-like polypeptide (HELP) as hybrid collagen-elastin matrices. In particular, we studied the biomechanical properties of collagen/HELP scaffolds considering several components involved in ECM remodeling (elastin, collagen, fibrillin, lectin-like receptor, metalloproteinases) and cell phenotype (myogenin, myosin heavy chain) with particular awareness for vascular tissue engineering applications. Elastin and collagen content resulted upregulated in collagen-HELP matrices, even showing an improved structural remodeling through the involvement of proteins to a ECM remodeling activity. Moreover, the hybrid matrices enhanced the contractile activity of C2C12 cells concurring to improve the mechanical properties of the scaffold. Finally, small-angle X-ray scattering analyses were performed to enable a very detailed analysis of the matrices at the nanoscale, comparing the scaffolds with native blood vessels. In conclusion, our work shows the use of recombinant HELP, as a very promising complement able to significantly improve the biomechanical properties of three-dimensional collagen matrices in terms of tensile stress and elastic modulus.

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Section: Introductionmentioning

confidence: 59%

Human elastin polypeptides improve the biomechanical properties of three‐dimensional matrices through the regulation of elastogenesis

Boccafoschi

Ramella

Sibillano

et al. 2014

J Biomedical Materials Res

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“…79 Their results showed that the surface-modi¯ed¯lms supported enhanced smooth muscle cell adhesion and functionality compared to the un-modi¯ed controls. These positive¯nds were attributed to the larger surface contact area achieved by the nano¯ber coatings for the cell attachment and nanoscale topographical architecture.…”

Section: Vascular Tissue Engineeringmentioning

confidence: 96%

Nano and Micro-Structures of Elastin-Like Polypeptide-Based Materials and Their Applications: Recent Developments

et al. 2013

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Elastin-like polypeptide (ELP) containing materials have spurred signi¯cant research interest for biomedical applications exploiting their biocompatible, biodegradable and nonimmunogenic nature while maintaining precise control over their chemical structure and functionality through genetic engineering. Physical, mechanical and biological properties of ELPs could be further manipulated using genetic engineering or through conjugation with a variety of chemical moieties. These chemical and physical modi¯cations also achieve interesting micro-and nanostructured ELPbased materials. Here, we review the recent developments during the past decade in the methods to engineer elastin-like materials, available genetic and chemical modi¯cation methods and applications of ELP micro and nanostructures in tissue engineering and drug delivery.

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“…36,37 In addition, the aligned fiber orientations also promote biological markers of a contractile phenotype including a spindle-like morphology, actin filament organization as well as SM-MHC expression. 38 For osteogenic differentiation, the aligned direction and topographic cues are reported to enhance proliferation and osteogenic differentiation of mesenchymal stem cells (MSC) compared to their random counterparts. [39][40][41] In addition, the osteogenic commitment of MSC is greatly dependent on the size scale of topographic cue.…”

Section: Uany Support Hadsc Viability and Alignmentmentioning

confidence: 99%

Fabrication of Aligned Nanofiber Polymer Yarn Networks for Anisotropic Soft Tissue Scaffolds

Duan

Liu

et al. 2016

ACS Appl. Mater. Interfaces

108

107

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Nanofibrous scaffolds with defined architectures and anisotropic mechanical properties are attractive for many tissue engineering and regenerative medicine applications. Here, a novel electrospinning system is developed and implemented to fabricate continuous processable uniaxially aligned nanofiber yarns (UANY). UANY were processed into fibrous tissue scaffolds with defined anisotropic material properties using various textile-forming technologies, i.e., braiding, weaving, and knitting techniques. UANY braiding dramatically increased overall stiffness and strength compared to the same number of UANY unbraided. Human adipose derived stem cells (HADSC) cultured on UANY or woven and knitted 3D scaffolds aligned along local fiber direction and were >90% viable throughout 21 days. Importantly, UANY supported biochemical induction of HADSC differentiation toward smooth muscle and osteogenic lineages. Moreover, we integrated an anisotropic woven fiber mesh within a bioactive hydrogel to mimic the complex microstructure and mechanical behavior of valve tissues. Human aortic valve interstitial cells (HAVIC) and human aortic root smooth muscle cells (HASMC) were separately encapsulated within hydrogel/woven fabric composite scaffolds for generating scaffolds with anisotropic biomechanics and valve ECM like microenvironment for heart valve tissue engineering. UANY have great potential as building blocks for generating fiber-shaped tissues or tissue microstructures with complex architectures.

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Electrospun elastin-like polypeptide enriched polyurethanes and their interactions with vascular smooth muscle cells

Cited by 47 publications

References 37 publications

Human elastin polypeptides improve the biomechanical properties of three‐dimensional matrices through the regulation of elastogenesis

Human elastin polypeptides improve the biomechanical properties of three‐dimensional matrices through the regulation of elastogenesis

Nano and Micro-Structures of Elastin-Like Polypeptide-Based Materials and Their Applications: Recent Developments

Fabrication of Aligned Nanofiber Polymer Yarn Networks for Anisotropic Soft Tissue Scaffolds

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