Electrospun decellularized extracellular matrix scaffolds promote the regeneration of injured neurons

Mungenast, Lena; Nieminen, Riina; Gaiser, Carine; Faia-Torres, Ana Bela; Rühe, Jürgen; Suter‐Dick, Laura

doi:10.1016/j.bbiosy.2023.100081

Cited by 6 publications

(3 citation statements)

References 81 publications

(105 reference statements)

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“…Soft surfaces have been shown to reduce the activity of stress actin filaments in cells [ 40 , 50 – 52 ]. Moreover, ES fibers have a structure resembling skin´s own extracellular matrix structure and the bioactivity of the cells changes when grown on ES fiber material [ 53 , 54 ]. Because fewer activated actin filaments can be seen in the confocal microscopy images (Figs 6 and 7 ), we can conclude that the surface of the tested ES fiber mat is more suitable for cell growth than glass.…”

Section: Discussionmentioning

confidence: 99%

In vitro experimental conditions and tools can influence the safety and biocompatibility results of antimicrobial electrospun biomaterials for wound healing

Põhako-Palu,

Lorenz,

Randmäe

et al. 2024

PLoS ONE

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Electrospun (ES) fibrous nanomaterials have been widely investigated as novel biomaterials. These biomaterials have to be safe and biocompatible; hence, they need to be tested for cytotoxicity before being administered to patients. The aim of this study was to develop a suitable and biorelevant in vitro cytotoxicity assay for ES biomaterials (e.g. wound dressings). We compared different in vitro cytotoxicity assays, and our model wound dressing was made from polycaprolactone and polyethylene oxide and contained chloramphenicol as the active pharmaceutical ingredient. Baby Hamster Kidney cells (BHK-21), human primary fibroblasts and MTS assays together with real-time cell analysis were selected. The extract exposure and direct contact safety evaluation setups were tested together with microscopic techniques. We found that while extract exposure assays are suitable for the initial testing, the biocompatibility of the biomaterial is revealed in in vitro direct contact assays where cell interactions with the ES wound dressing are evaluated. We observed significant differences in the experimental outcome, caused by the experimental set up modification such as cell line choice, cell medium and controls used, conducting the phosphate buffer washing step or not. A more detailed technical protocol for the in vitro cytotoxicity assessment of ES wound dressings was developed.

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Section: Discussionmentioning

confidence: 99%

In vitro experimental conditions and tools can influence the safety and biocompatibility results of antimicrobial electrospun biomaterials for wound healing

Põhako-Palu,

Lorenz,

Randmäe

et al. 2024

PLoS ONE

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“…Among these examples, precision engineering of dECM is emerging as a key area of focus, with advanced biofabrication techniques, including 3D bioprinting (Liu, Gong, et al, 2023; Tang et al, 2021; Wang et al, 2022) and electrospinning (Mungenast et al, 2023), on track to accelerate the development of dECM therapeutic solutions. These methods will enable the creation of patient‐specific dECM scaffolds that precisely replicate the complex architecture and composition of individual CNS tissues.…”

Section: Matrices For Neural Applicationsmentioning

confidence: 99%

Exploring the properties and potential of the neural extracellular matrix for next‐generation regenerative therapies

Ortega,

Soares de Aguiar,

Chandravanshi

et al. 2024

WIREs Nanomed Nanobiotechnol

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The extracellular matrix (ECM) is a dynamic and complex network of proteins and molecules that surrounds cells and tissues in the nervous system and orchestrates a myriad of biological functions. This review carefully examines the diverse interactions between cells and the ECM, as well as the transformative chemical and physical changes that the ECM undergoes during neural development, aging, and disease. These transformations play a pivotal role in shaping tissue morphogenesis and neural activity, thereby influencing the functionality of the central nervous system (CNS). In our comprehensive review, we describe the diverse behaviors of the CNS ECM in different physiological and pathological scenarios and explore the unique properties that make ECM‐based strategies attractive for CNS repair and regeneration. Addressing the challenges of scalability, variability, and integration with host tissues, we review how advanced natural, synthetic, and combinatorial matrix approaches enhance biocompatibility, mechanical properties, and functional recovery. Overall, this review highlights the potential of decellularized ECM as a powerful tool for CNS modeling and regenerative purposes and sets the stage for future research in this exciting field.This article is categorized under: Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement Therapeutic Approaches and Drug Discovery > Nanomedicine for Neurological Disease Implantable Materials and Surgical Technologies > Nanomaterials and Implants

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“…Thanks to the recent progress registered in the fields of materials science and TE, it is possible to modulate the physicochemical and biological properties to generate a biobased scaffold for various applications, from antibacterial surfaces [60] and engineered bacteria [61] to tissue regeneration [62][63][64][65][66] and wound healing [17,67,68]. Particularly, cellbiomaterial interactions are crucial to determine the cellular fate in terms of adhesion [69,70], proliferation [71], differentiation [72,73], morphology [74,75], migration [76,77], and for the ECM-mimetic scaffold fabrication [78,79].…”

Section: Modulation Of Cell Fate By Cell-biomaterials Interactionsmentioning

confidence: 99%

Cell-Tissue Interaction: The Biomimetic Approach to Design Tissue Engineered Biomaterials

Nitti,

Narayanan,

Pellegrino

et al. 2023

Bioengineering

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The advancement achieved in Tissue Engineering is based on a careful and in-depth study of cell–tissue interactions. The choice of a specific biomaterial in Tissue Engineering is fundamental, as it represents an interface for adherent cells in the creation of a microenvironment suitable for cell growth and differentiation. The knowledge of the biochemical and biophysical properties of the extracellular matrix is a useful tool for the optimization of polymeric scaffolds. This review aims to analyse the chemical, physical, and biological parameters on which are possible to act in Tissue Engineering for the optimization of polymeric scaffolds and the most recent progress presented in this field, including the novelty in the modification of the scaffolds’ bulk and surface from a chemical and physical point of view to improve cell–biomaterial interaction. Moreover, we underline how understanding the impact of scaffolds on cell fate is of paramount importance for the successful advancement of Tissue Engineering. Finally, we conclude by reporting the future perspectives in this field in continuous development.

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Electrospun decellularized extracellular matrix scaffolds promote the regeneration of injured neurons

Cited by 6 publications

References 81 publications

In vitro experimental conditions and tools can influence the safety and biocompatibility results of antimicrobial electrospun biomaterials for wound healing

In vitro experimental conditions and tools can influence the safety and biocompatibility results of antimicrobial electrospun biomaterials for wound healing

Exploring the properties and potential of the neural extracellular matrix for next‐generation regenerative therapies

Cell-Tissue Interaction: The Biomimetic Approach to Design Tissue Engineered Biomaterials

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