Electrospray mass spectrometry: a tool for elemental speciation

Stewart, Ian I.

doi:10.1016/s0584-8547(99)00110-x

Cited by 106 publications

(79 citation statements)

References 342 publications

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“…Organic mass spectrometry (such as ESI-MS, MALDI-TOF-MS) will be useful supplement for the speciation analysis and protein quantification purposes. 32,33 The unknown peaks will be studied with organic mass spectrometry in our further work.…”

Section: Resultsmentioning

confidence: 99%

Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Jeong¹,

Lee²,

Pak

2013

Bulletin of the Korean Chemical Society

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Various separation modes in HPLC, such as anion exchange (AE), reversed-phase (RP), and affinity (AF) chromatography were examined for the separation of selenium species in human blood serum and urine. While RP-and AE-HPLC were mainly used for the separation of small molecular selenium species, double column AF-HPLC achieved the separation of selenoproteins in blood serum efficiently. Further, the effluent of AF-HPLC was enzymatically hydrolyzed and then analyzed with RP HPLC for selenoamino acid study. The versatility of the hybrid technique makes the in-depth study of selenium species possible. For quantification, post column isotope dilution (ID) with 78Se spike was performed. ORC ICP/MS (octapole reaction cell inductively coupled plasma/mass spectrometry) was used with 4 mL min −1 Hydrogen as reaction gas. In urine sample, inorganic selenium and SeCys were identified. In blood serum, selenoproteins GPx, SelP and SeAlb were detected and quantified. The concentration for GPx, SelP and SeAlb was 22.8 ± 3.4 ng g Enzymatic hydrolysis of each selenium proteins revealed that SeCys is the major amino acid for all three proteins and SeMet is contained in SeAlb only.

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Section: Resultsmentioning

confidence: 99%

Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Jeong¹,

Lee²,

Pak

2013

Bulletin of the Korean Chemical Society

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“…Extremely advantageous is the ability of this technique to allow for the conversion of a liquid sample into its gaseous form, thereby ensuring that little of the original species information is lost [100]. It is especially useful for organic solvents which often pose problems in ICP-MS, and is used in the identification of unfragmented molecules, where further fragmentation can be induced and controlled through collisions (Collision Induced Dissociation; CID) [1].…”

Section: Electrospray Ionization Mass Spectrometry (Esi-ms)mentioning

confidence: 99%

“…An undesirable effect in this type of analysis is the formation of ion-solvent clusters, where a single species may be split into multiple signals due to the solvent forming a gas and expanding in the vacuum [100]. This, along with electrolytic processes occurring at the tip of the ESI needle, may result in the transformation of chemical species [1].…”

Section: Electrospray Ionization Mass Spectrometry (Esi-ms)mentioning

confidence: 99%

Metal and metalloid speciation in plants: Overview, instrumentation, approaches and commonly assessed elements

Kroukamp

Wondimu

Forbes

2016

TrAC Trends in Analytical Chemistry

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The ability of plants to hyper-accumulate metals and metalloids from the surrounding environment may pose a significant health risk to both humans and animals since plants form a substantial component of diet. This attribute, however, has also been identified as a useful tool in bioremediation and biomonitoring studies; where assimilated metal(loid)s in plants often correlate to environmental exposure. Since the bioavailability and toxicity of these elements depends upon their chemical form, speciation studies are essential in determining mobility and metabolic pathways. This can be done in a number of ways where sampling, pre-treatment and storage are all important factors affecting speciation. Appropriate analytical techniques for speciation studies can either be direct methods such as XAS, or indirect methods which require species separation prior to analysis. Separation techniques can be in the form of sequential extractions or column separation and analyte detection often utilises instrumentation such as ESI-MS, ICP-MS and ICP-OES.

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“…Therefore, interference can potentially occur with Mn (m/z 55) from 40 [26]. The polyatomic ions which can interfere in the ICP-MS detection of these elements can be partly reduced by the manipulation of parameters such as the carrier gas flow rate and the forward power.…”

Section: Icp-ms Detection Of Metal -Edta Complexesmentioning

confidence: 99%

Speciation of metal–EDTA complexes by flow injection analysis with electrospray ionization mass spectrometry and ion chromatography with inductively coupled plasma mass spectrometry

Chen¹,

Sun

et al. 2008

J of Separation Science

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Original PaperSpeciation of metal-EDTA complexes by flow injection analysis with electrospray ionization mass spectrometry and ion chromatography with inductively coupled plasma mass spectrometry Flow injection analysis (FIA) with ESI-MS and ion chromatography (IC) with inductively coupled plasma-MS (ICP-MS) and their MS spectral showed that these metal -EDTA complexes were present in solution. Based on the ESI-MS, ion chromatographic separation and ICP-MS detection of these complexes are possible because IC-ICP-MS requires stable metal -EDTA complex during the chromatographic separation. The separation of these metal -EDTA complexes was achieved on an anion-exchange column with a mobile phase containing 30 mM NH 4 (HPO 4 ) 2 at pH 7.5 within 7 min with ICP-MS providing element specific detection. The ICP-MS LODs for the metal -EDTA were in the range of 0.1 -0.5 lg/L with the exception of Fe (15 lg/L). The proposed method was a simple procedure for sample processing, using direct injection of sample without removal of sample matrix and was successfully applied to the determination of metal -EDTA complexes in real samples. IntroductionEDTA is a chelating agent which has the potential to perturb the natural speciation of metals, to influence metal bioavailability [1], and to remobilize metals from sediments and aquifers [2]. Chelating agents occur in natural waters predominantly as metal complexes. There are many studies emphasizing the importance of chelation on metal bioavailability, plant uptake, toxicity, transport, adsorption, distribution, and fate [3]. However, the identification and detection of these metal complexes in environmental sample is still a significant analytical challenge because of their structural similarities and presence at low concentrations in environmental matrices [4]. Separation of these complexes is therefore an important step in the development of an analytical method suitable for the detection of metal -EDTA complexes in environmental samples.CE and ion chromatography (IC) are the main separation methods used for the determination of metal -aminopolycarboxylic complexes. Using the CE approach, metal complexes with nitrilotriacetic acid (NTA), hydroxyethylethylenediaminetriacetic acid (HEDTA), EDTA, and diethylenetriaminepentaacetic acid (DTPA) were all successfully separated using either CZE [5 -7] or MEKC [8] with UV detection. LODs of 2 -500 lM were obtained using these CE methods, which are usually not sensitive enough for the analysis of trace levels of these complexes in environmental samples. As an alternative to CE, IC is often used to determine metal -EDTA complexes, based on the precolumn complexation of the chelating agents with metals. The separation of these complexes by ionpairing [9 -12] and ion-exchange chromatography [13 -15] has been accomplished with conductivity or UV/Visible detection, but these detectors are nonselective and it is therefore often impossible to identify all metal -EDTA complexes in complex matrices. To address these problems, the separation...

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Electrospray mass spectrometry: a tool for elemental speciation

Cited by 106 publications

References 342 publications

Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Metal and metalloid speciation in plants: Overview, instrumentation, approaches and commonly assessed elements

Speciation of metal–EDTA complexes by flow injection analysis with electrospray ionization mass spectrometry and ion chromatography with inductively coupled plasma mass spectrometry

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