Electrospray liquid chromatography-mass spectrometry of the leaf extract of Rhamnus prinoides

Nindi, Mathew Muzi; Kgarebe, Boitumelo; Wolfender, Jean‐Luc; Abegaz, Berhanu M.

doi:10.1002/(sici)1099-1565(199903/04)10:2<69::aid-pca447>3.0.co;2-4

Cited by 18 publications

(5 citation statements)

References 7 publications

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“…As for Peak 26 , the [M − H] − ion at m / z 299 was obtained, which further generated MS/MS fragment ions [M − H − CH 3 ] − at m / z 284, [M − H − CH 3 − CO] − at m / z 256, [M − H − CH 3 − CO 2 ] − at m / z 240 and [M − H − CH 3 − CO − CHO] − at m / z 227. Compared with those ESI-MS/MS spectra and fragmentation pathways, Peak 26 was thus identified as rhamnocitrin (calculated for C 16 H 12 O 6 , m / z 300) [11,31]. Peak 32 presented the [M − H] − ion at m / z 359, and its MS/MS fragment ions at m / z 285, 267, 241 and 223 were similar to that of Peak 18 , which suggested Peak 32 as a luteolin derivative.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Flavonoids in Rhamnus davurica and Its Antiproliferative Activities

et al. 2016

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Rhamnus davurica Pall. (R. davurica) has been used as a traditional medicinal herb for many years in China and abroad. It has been well documented as a rich source of flavonoids with diversified structures, which in turn results in far-ranging biological activities, such as anti-inflammation, anticancer, antibacterial and antioxidant activities. In order to further correlate their anticancer potentials with the phytochemical components, the fingerprint profile of R. davurica herb from Dongbei was firstly investigated using HPLC-ESI-MS/MS. Thirty two peaks were detected and identified, 14 of which were found in R. davurica for the first time in this work. Furthermore, a total of 23 peaks were resolved as flavonoids, which are the major components found in R. davurica. Meanwhile, the antiproliferative activities against human cancer cells of HT-29 and SGC-7901 in vitro exhibited distinct inhibitory effects with IC 50 values at 24.96 ± 0.74 and 89.53 ± 4.11 µg/mL, respectively. Finally, the general toxicity against L-O2 cells displayed a much higher IC 50 at 229.19 ± 8.52 µg/mL, which suggested very low or no toxicity on hepatic cell viability. The current study revealed for the first time the correlations between the flavonoids of R. davurica with their antiproliferative activities, which indicated that the fingerprint profile of flavonoids and their anticancer activities could provide valuable information on the quality control for herbal medicines and their derived natural remedies from this valuable medicinal plant.

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Section: Resultsmentioning

confidence: 99%

Analysis of Flavonoids in Rhamnus davurica and Its Antiproliferative Activities

et al. 2016

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“…As shown in Figure 2 above, 65 peaks were detected in SPRE. Further structural identification and characterization of those compounds in Table 3 were carried out strictly by comparison with the chemical standards or the MS fragments reported in previous studies [12][13][14][15]23,37,38] On this basis, further studies on spectrum-effect should also be carried out to truly correlate its QC with its clinical efficacy, and help to clarify the mechanisms of action. For the relative quantitative analysis of chemicals in SPRE, the aforementioned flavonoids, phenols, naphthols, anthraquinones, phenylpropanoid, saponin, and their glycosides take up 69.54%, 2.39%, 17.87%, 6.39%, 0.53%, 0.98%, respectively.…”

Section: Hplc-uv/esi-ms/ms Analysismentioning

confidence: 99%

“…As shown in Figure 2 above, 65 peaks were detected in SPRE. Further structural identification and characterization of those compounds in Table 3 were carried out strictly by comparison with the chemical standards or the MS fragments reported in previous studies [12][13][14][15]23,37,38]. As a result, several types of polyphenols, including flavonoids and their glycosides (peaks 3-9, 11-15, 18, 19, 21, 22, 24-26, 28-31, 34, 37, 38, 40-42, 44-50, 53, 54, 56-63, 65), phenols and their glycosides (peaks 1, 2, 20, 23), naphthols and their glycosides (peaks 16, 36, 39, 43, 51, 52), anthraquinones and their glycosides (peaks 17, 33, 35, 64), phenylpropanoid glycoside (peak 55), and saponin (peak 32), are revealed majorly in R. prinoides.…”

Section: Hplc-uv/esi-ms/ms Analysismentioning

confidence: 99%

“…Except for the essential and non-essential metals, volatile oils, and saponins with lower content, present phytochemical studies indicated that R. prinoides mainly contains varieties of phenolic compounds, including flavonoids, anthraquinones, naphthols, and their glycosides [1,[12][13][14][15]. For example, the geshoidin, a naphthalene glycoside (β-sorigenin-8-O-β-D-glucoside), is one of the most important compounds in this plant, and partially responsible for the characteristic bitterness in the popular beverages of Tella.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant, Anti-inflammatory Activities and Polyphenol Profile of Rhamnus prinoides

Chen

Mutie

et al. 2020

Pharmaceuticals

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Rhamnus prinoides L’Herit (R. prinoides) has long been widely consumed as folk medicine in Kenya and other Africa countries. Previous studies indicated that polyphenols were abundant in genus Rhamnus and exhibited outstanding antioxidant and anti-inflammatory activities. However, there are very few studies on such pharmacological activities and the polyphenol profile of this plant up to now. In the present study, the antioxidant activities of the crude R. prinoides extracts (CRE) and the semi-purified R. prinoides extracts (SPRE) of polyphenol enriched fractions were evaluated to show the strong radical scavenging effects against 1,1-diphenyl-2- picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) (0.510 ± 0.046 and 0.204 ± 0.005, mg/mL), and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) (0.596 ± 0.005 and 0.096 ± 0.004, mg/mL), respectively. Later, the SPRE with higher contents of polyphenols and flavonoids displayed obvious anti-inflammatory activities through reducing the NO production at the dosage of 11.11 − 100 μg/mL, and the COX-2 inhibitory activity with an IC50 value at 20.61 ± 0.13 μg/mL. Meanwhile, the HPLC-UV/ESI-MS/MS analysis of polyphenol profile of R. prinoides revealed that flavonoids and their glycosides were the major ingredients, and potentially responsible for its strong antioxidant and anti-inflammatory activities. For the first time, the present study comprehensively demonstrated the chemical profile of R. prinoides, as well as noteworthy antioxidant and anti-inflammatory activities, which confirmed that R. prinoides is a good natural source of polyphenols and flavonoids, and provided valuable information on this medicinal plant as folk medicine and with good potential for future healthcare practice.

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“…R. prinoides is a dense shrub or small tree which grows up to 6 meters tall. It is one of the two species that represent the genus Rhamnus and a widespread plant species in Africa [2]. e plant is native to Ethiopia, Eritrea, Botswana, Namibia, Lesotho, the treatment of atopic dermatitis [6].…”

Section: Introductionmentioning

confidence: 99%

Assessment and Determination of Bittering Agents, Essential Oils, and Antioxidants of Gesho (Rhamnus prinoides L. Herit) Collected from Amhara Region, Ethiopia

Negash

Tilahun

Tsehai

2021

Journal of Chemistry

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Rhamnus prinoides (Gesho) is an angiosperm dioecious plant, which is used for cash income, as a bittering agent for the preparation of the local alcoholic beverage and medicinal values. Although it has intense use, still its practical application is limited for local alcoholic beverage preparation, and there is no sufficient scientific report on the level and quantity of bittering agents, essential oils, and antioxidants of this plant. Therefore, the objective of this research was to assess and determine the bittering agents, essential oils, and antioxidants of R. prinoides. The leaves of R. prinoides were collected from eleven different sample sites and allowed to sun dry and then powdered. It was further dried in an oven at 60°C for 1 h, and then, total resin, essential oils, alpha acid, iso-alpha acid, beta acid, flavonoids, and polyphenols of R. prinoides were determined. All the data were analyzed using SPSS version 20.0. Based on this study finding, (16.03 ± 0.03–17.05 ± 0.04%) total resins, (10.00 ± 0.08–11.23 ± 0.07%) soft resins, (5.65 ± 0.11–6.40 ± 0.35%) hard resins, (1.82 ± 0.11–3.14 ± 0.02 mg/l) alpha acids, (2.26 ± 0.15–4.15 ± 0.10 mg/l) iso-alpha acids, (2.62 ± 0.04–4.29 ± 0.08 mg/l) beta acids, (7.74 ± 0.11–12.47 ± 0.13%) essential oils, (23.00 ± 0.14–25.01 ± 0.09%) flavonoids, and (2.11 ± 0.01–2.41 ± 0.02%) polyphenols were obtained in the leaves of R. prinoides. R. prinoides leaves taken from Tikledingay has shown statistically ( P ≤ 0.05 ) significant amount of soft resin (11.23 ± 0.07%), alpha acid (3.14 ± 0.02 mg/l), iso-alpha acid (4.15 ± 0.10 mg/l), essential oils (12.47 ± 0.13%), flavonoids (25.01 ± 0.09%), and polyphenols (2.41 ± 0.02%) in comparison with other samples. Generally, R. prinoides is a good source of bittering agents, essential oils, and antioxidants. However, the quantity of bittering agents, essential oils, and antioxidants in the leaves of R. prinoides was varied with sample collection sites. So R. prinoides can be used as a substitute for commercial hops used in the breweries, and promotion should be done to introduce the potential of Gesho as a bittering agent, source of aroma and flavor, and also antioxidant for beer and other alcoholic beverages.

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Electrospray liquid chromatography-mass spectrometry of the leaf extract of Rhamnus prinoides

Cited by 18 publications

References 7 publications

Analysis of Flavonoids in Rhamnus davurica and Its Antiproliferative Activities

Analysis of Flavonoids in Rhamnus davurica and Its Antiproliferative Activities

Antioxidant, Anti-inflammatory Activities and Polyphenol Profile of Rhamnus prinoides

Assessment and Determination of Bittering Agents, Essential Oils, and Antioxidants of Gesho (Rhamnus prinoides L. Herit) Collected from Amhara Region, Ethiopia

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