Electrospray liquid chromatography-mass spectrometry of the leaf extract of Rhamnus prinoides

Analysis of Flavonoids in Rhamnus davurica and Its Antiproliferative Activities

“…As for Peak 26 , the [M − H] − ion at m / z 299 was obtained, which further generated MS/MS fragment ions [M − H − CH 3 ] − at m / z 284, [M − H − CH 3 − CO] − at m / z 256, [M − H − CH 3 − CO 2 ] − at m / z 240 and [M − H − CH 3 − CO − CHO] − at m / z 227. Compared with those ESI-MS/MS spectra and fragmentation pathways, Peak 26 was thus identified as rhamnocitrin (calculated for C 16 H 12 O 6 , m / z 300) [11,31]. Peak 32 presented the [M − H] − ion at m / z 359, and its MS/MS fragment ions at m / z 285, 267, 241 and 223 were similar to that of Peak 18 , which suggested Peak 32 as a luteolin derivative.…”

Antioxidant, Anti-inflammatory Activities and Polyphenol Profile of Rhamnus prinoides

“…As shown in Figure 2 above, 65 peaks were detected in SPRE. Further structural identification and characterization of those compounds in Table 3 were carried out strictly by comparison with the chemical standards or the MS fragments reported in previous studies [12][13][14][15]23,37,38] On this basis, further studies on spectrum-effect should also be carried out to truly correlate its QC with its clinical efficacy, and help to clarify the mechanisms of action. For the relative quantitative analysis of chemicals in SPRE, the aforementioned flavonoids, phenols, naphthols, anthraquinones, phenylpropanoid, saponin, and their glycosides take up 69.54%, 2.39%, 17.87%, 6.39%, 0.53%, 0.98%, respectively.…”

“…As shown in Figure 2 above, 65 peaks were detected in SPRE. Further structural identification and characterization of those compounds in Table 3 were carried out strictly by comparison with the chemical standards or the MS fragments reported in previous studies [12][13][14][15]23,37,38]. As a result, several types of polyphenols, including flavonoids and their glycosides (peaks 3-9, 11-15, 18, 19, 21, 22, 24-26, 28-31, 34, 37, 38, 40-42, 44-50, 53, 54, 56-63, 65), phenols and their glycosides (peaks 1, 2, 20, 23), naphthols and their glycosides (peaks 16, 36, 39, 43, 51, 52), anthraquinones and their glycosides (peaks 17, 33, 35, 64), phenylpropanoid glycoside (peak 55), and saponin (peak 32), are revealed majorly in R. prinoides.…”

“…Except for the essential and non-essential metals, volatile oils, and saponins with lower content, present phytochemical studies indicated that R. prinoides mainly contains varieties of phenolic compounds, including flavonoids, anthraquinones, naphthols, and their glycosides [1,[12][13][14][15]. For example, the geshoidin, a naphthalene glycoside (β-sorigenin-8-O-β-D-glucoside), is one of the most important compounds in this plant, and partially responsible for the characteristic bitterness in the popular beverages of Tella.…”

Assessment and Determination of Bittering Agents, Essential Oils, and Antioxidants of Gesho (Rhamnus prinoides L. Herit) Collected from Amhara Region, Ethiopia

“…R. prinoides is a dense shrub or small tree which grows up to 6 meters tall. It is one of the two species that represent the genus Rhamnus and a widespread plant species in Africa [2]. e plant is native to Ethiopia, Eritrea, Botswana, Namibia, Lesotho, the treatment of atopic dermatitis [6].…”