Electrospray Ionization Mass Spectrometry: A Complementary Source for Trace Element Speciation Analysis

Hansen, Hanna E.; Pergantis, Spiros A.

doi:10.1002/9780470988794.ch8

Cited by 1 publication

(2 citation statements)

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“…However, restrictions in the analytical accessibility constitute another reason. So far, advances within the area of antimony speciation have been limited, compared to advances in speciation of other metalloids such as arsenic and selenium . Understanding the detailed chemical structure of a drug is essential in order to establish its mechanism of efficiency and to device further improvements in drug development, with reduced side effects.…”

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confidence: 99%

Characterization of Sodium Stibogluconate by Online Liquid Separation Cell Technology Monitored by ICPMS and ESMS and Computational Chemistry

Hansen

Jensen

et al. 2008

Anal. Chem.

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High-performance liquid chromatography (HPLC), mass spectrometry (MS), and computational chemistry has been applied to resolve the composition and structure of the Sb species present in dilutions of Pentostam, a first-line treatment drug against Leishmania parasites. Using HPLC-inductively coupled plasma-MS and electrospray-MS, it was shown that the original drug consists of large Sb(V)-glyconate complexes of polymeric nature that degrade upon dilution. In dilution solution, the drug is a mixture of noncomplexed Sb(V), large polymeric complexes as well as several low molecular mass Sb(V)-glyconate complexes of various stoichiometry (1:1, 1:2, 1:3, 2:2, 2:3, 2:4, 3:3, 3:4). The 1:1 complex became the most abundant low molecular mass Sb(V) complex with dilution time. A novel mixed-mode chromatographic system was applied in order to separate complexes of various stoichiometry and isomers. Density functional theory was used to study the structure of the 1:1 Sb-gluconate complex with three or four solvent molecules bound. By computing the structures and the free energies of the various possible isomers in aqueous solvation models, the most likely structures of the species were deduced. Importantly, 6-coordination is always preferred over 5-coordination, and the species commonly adopt conformations involving tris-coordination of deprotonated hydroxyl groups from gluconate.

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confidence: 99%

Characterization of Sodium Stibogluconate by Online Liquid Separation Cell Technology Monitored by ICPMS and ESMS and Computational Chemistry

Hansen

Jensen

et al. 2008

Anal. Chem.

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“…Techniques including fastatom bombardment, thermospray and desorption chemical ionization MS have been used with varying degrees of success for speciation analysis of non-volatile species. 194 Electrospray ionization MS (ESMS) was shown to be superior to other ionization sources due to its direct and efficient generation of gas-phase ions of inorganic, organometallic and organometalloid compounds, 195 large biomolecules and non-covalent biomolecular complexes; thus allowing for the study of non-covalent metal-biomolecule interactions. 196 Since the mid 90's ESMS has been applied with great success for element speciation analyses within the research areas of environmental chemistry, health and nutrition and bioinorganic chemistry 197 and has contributed to the identification and characterisation of a large number of metallothioneins, phytochelatins and anthropogenic contaminants 198 and other elemental species in biochemistry.…”

Section: The Role Of Molecular Mass Spectrometrymentioning

confidence: 99%