Electrospray ionization LC–MS/MS validated method to quantify griseofulvin in human plasma and its application to bioequivalence study

Mistri, Hiren N.; Jangid, Arvind G.; Sanyal, Mallika; Shrivastav, Pranav S.

doi:10.1016/j.jchromb.2006.12.003

Cited by 15 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method involves a single step acetonitrile extraction of griseofulvin from rat plasma. The limits of detection (LOD) and quantification (LOQ) are greatly improved compared to other reported HPLC methods for griseofulvin in biological media [7–10] and are comparable to the LC/MS/MS method of Mistri and coworkers [11]. …”

Section: Introductionmentioning

confidence: 94%

Development and Validation of a HPLC Method to Determine Griseofulvin in Rat Plasma: Application to Pharmacokinetic Studies

2008

View full text Add to dashboard Cite

A simple, specific, sensitive, and rapid high performance liquid chromatography (HPLC) method for the determination of griseofulvin in small volumes of rat plasma was developed and validated using warfarin as an internal standard. Biological sample preparation involved simple extraction with acetonitrile, followed by dilution with aqueous mobile phase buffer (20 mM sodium dihydrogen phosphate, pH 3.5) to eliminate any chromatographic solvent effects. Griseofulvin and warfarin were baseline separated and quantitated on a C18 reversed phase column (4.6 × 150 mm, 3.5 μm), using a mobile phase composed of a 20 mM aqueous solution of sodium dihydrogen phosphate-acetonitrile (55:45, v/v, pH 3.5) delivered at a flow rate of 1.0 mL/min, and with fluorescence detection (λexcitation = 300 nm, λemission = 418 nm). The method was proven to be linear over a plasma griseofulvin concentration range of 10 to 2500 ng/mL with a mean correlation coefficient of 0.9996. The intra-day and inter-day accuracy (relative error) were in the range of 0.89% to 9.26% and 0.71% to 7.68%, respectively. The within-day precision (coefficient of variation) was less than 3.0% and the between-day precision was less than 7.5%. The mean recovery of griseofulvin from rat plasma was found to be 99.2%. The limit of detection (LOD) and the limit of quantification (LOQ) of griseofulvin were determined to be 1 ng/mL and 10 ng/mL, respectively. The developed method was successfully applied to quantitatively assess the pharmacokinetics of griseofulvin in rats following a single 50 mg/kg oral dose of the drug.

show abstract

Section: Introductionmentioning

confidence: 94%

Development and Validation of a HPLC Method to Determine Griseofulvin in Rat Plasma: Application to Pharmacokinetic Studies

2008

View full text Add to dashboard Cite

show abstract

“…As part of the investigation to elucidate the structures of the two candidate markers (M2a and M2c), the fragmentation of the griseofulvin [M + H] + ion was studied at varying collision cell energies. A fragmentation pathway for protonated griseofulvin had previously been suggested as part of an LC/MS/MS study . In this previous study five product ions were identified and formulae and structures postulated (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…In liver four metabolites, M2a and M2c Table 2. Literature and proposed product ion formulae for griseofulvin Literature [10] Proposed Mass accuracy/ ppm Metabolite profiling using LC/qToFMS wileyonlinelibrary.com/journal/rcm (both demethylated), M3b (demethylated + glucuronide) and M9 (demethylated + chlorine replaced), were detected at 3 DALA. M2a was still detected at 10 DALA, albeit at low response.…”

Section: Response Depletionmentioning

confidence: 99%

“…A fragmentation pathway for protonated griseofulvin had previously been suggested as part of an LC/MS/MS study. [10] In this previous study five product ions were identified and formulae and structures postulated ( Table 2). Of these five ions, two (m/z 321, 257) were not observed in the current study.…”

Section: Structural Elucidation From Fragmentation Experimentsmentioning

confidence: 99%

See 1 more Smart Citation

Metabolite profiling using liquid chromatography/quadrupole time‐of‐flight mass spectrometry for the identification of a suitable marker and target matrix of griseofulvin use in bovines

Tarbin

Fussell

2013

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…[11] The LC-MS=MS method was also applied to quantify other drugs, using propranolol as internal standard, in clinical pharmacokinetic studies. [12,13] The aim of the present work was to develop and validate a simple, fast, precise, and accurate LC-MS=MS method, using liquid-liquid extraction, for the pharmacokinetic analysis of propranolol in human plasma supporting the bioequivalence study of two pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Propranolol in Human Plasma and its Application to a Bioequivalence Study

Borges

Rigato

Oliveira

et al. 2008

Journal of Liquid Chromatography & Related Technologies

View full text Add to dashboard Cite

A rapid, sensitive, and specific method based on liquid chromatography= tandem mass spectrometry (LC-MS=MS) was developed and validated for the determination of propranolol in human plasma using metoprolol as internal standard. The drugs were extracted from plasma by liquid-liquid extraction and separated isocratically on a Phenomenex Synergi Fusion-RP C 18 analytical column, 4 mm (150 mm Â 4.6 mm i.d.) maintained at 30 C, with acetonitrile=water (95=5, v=v): 100 mM ammonium acetate: 100 mM acetic acid (65:15:20 v=v=v) as mobile phase, run at a flow rate of 1 mL min À1 (split 1:3). Detection was carried out by positive electrospray ionization (ESIþ) in selected reaction monitoring (SRM) mode. The chromatographic separation was obtained within 3.0 min and was linear in the concentration range of 2-150 ng mL À1 (r 2 ¼ 0.9969). The method was successfully applied for the bioequivalence study of two tablet Correspondence: Sérgio Luiz Dalmora, formulations (test and reference) of propranolol 80 mg after single oral dose administration to 36 healthy human volunteers, using an open, randomized, two period crossover design with one week wash out interval.The geometric means ratios of C max and AUC (0-1) were 99.77 and 103.70%, respectively, with both the confidence intervals between 90.12-112.92% demonstrating the bioequivalence of the two formulations.

show abstract

Electrospray ionization LC–MS/MS validated method to quantify griseofulvin in human plasma and its application to bioequivalence study

Cited by 15 publications

References 20 publications

Development and Validation of a HPLC Method to Determine Griseofulvin in Rat Plasma: Application to Pharmacokinetic Studies

Development and Validation of a HPLC Method to Determine Griseofulvin in Rat Plasma: Application to Pharmacokinetic Studies

Metabolite profiling using liquid chromatography/quadrupole time‐of‐flight mass spectrometry for the identification of a suitable marker and target matrix of griseofulvin use in bovines

Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Propranolol in Human Plasma and its Application to a Bioequivalence Study

Contact Info

Product

Resources

About