Electrospinning versus microfluidic spinning of functional fibers for biomedical applications

Cheng, Jie; Jun, Yesl; Qin, Jianhua; Lee, Sang Hoon

doi:10.1016/j.biomaterials.2016.10.040

Cited by 295 publications

(246 citation statements)

References 254 publications

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“…Current methods of preparing fibers mainly include melt spinning,14 wet spinning,15,16 and electrospinning 17–19. However, the presence of high temperatures and volatile solvents in the melt spinning and electrospinning processes limits the encapsulation of cells, microtissues, and other bioactive molecules in fibers 20. For example, electrospinning microfibers must be removed with organic solvents before they can be used as a substrate for cell culture, which limits the construction of more complex 3D tissues.…”

Section: Introductionmentioning

confidence: 99%

Simple Fabrication of Multicomponent Heterogeneous Fibers for Cell Co‐Culture via Microfluidic Spinning

Yao

et al. 2020

Macromolecular Bioscience

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Microfluidic spinning, as a combination of wet spinning and microfluidic technology, has been used to develop microfibers with special structures to facilitate cell 3D culture/co‐culture and microtissue formation in vitro. In this study, a simple microchip‐based microfluidic spinning strategy is presented for the fabrication of multicomponent heterogeneous calcium alginate microfibers. The use of two kinds of microchip enables the one‐step preparation of multicomponent heterogeneous microfibers with various arrangement patterns, including the preparation of one‐, two‐, and three‐component microfibers by a two‐layer microchip and preparation of four component microfibers with different arrangement by a membrane‐sandwiched three‐layer microchip. The obtained microfibers could be used to encapsulate various kinds of cells, such as the human non‐small cell lung cancer cell NCI‐H1650, the human fetal lung fibroblast HFL1, the normal pulmonary bronchial epithelial cell 16HBE, and human umbilical vein endothelial cells. By adding chitosan to the medium to keep the fibers stable, 3D long‐term in vitro cell co‐culture has been carried out up to 21 days. This method is very simple and easy to operate, continuously produces spatially well‐defined heterogeneous microfibers, has important applications for composite functional biomaterials, and shows great potential in organs‐on‐a‐chip and biomimetic systems.

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Section: Introductionmentioning

confidence: 99%

Simple Fabrication of Multicomponent Heterogeneous Fibers for Cell Co‐Culture via Microfluidic Spinning

Yao

et al. 2020

Macromolecular Bioscience

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“…[ 16,17,19,20 ] The first commonly used approach is the electrospinning technique that has been intensively applied due to the advantages of reproducibility, simplicity, and diversity in producing fibers with micro‐ or nanoscale diameters and different topographical features. [ 21,22 ] The fibers' membrane produced by electrospinning can provide large surface areas for cells to attach and proliferate, which could also enhance the penetration of nutrients and oxygen. [ 23,24 ] However, electrospinning is hard to fabricate 3D structure due to the nanoscale of fibers, which results in 2D structures in most cases.…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

Liu

Zhang

et al. 2020

Macro Materials & Eng

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Skin injuries are an urgent health issue, which raises a great concern in the clinic. Although numerous strategies have been proposed to fabricate skin substitutes for treatment of wounds over the past several decades, fabricating an ideal skin substitute to replace the damaged one can still be a problem. In this study, a novel biomimetic 3D composite skin scaffold is fabricated by combining electrohydrodynamic (EHD) jetting, electrospinning, and coating techniques. Here, the first polycaprolactone (PCL) porous structure is produced by the EHD jetting. Next, the second polylactic acid (PLA) membrane consisted of nanoscale fibers is prepared on the PCL porous structure via the electrospinning. The PCL porous structure and PLA fibers membrane can mimic the dermis and epidermis layer, respectively. Furthermore, gelatin is used as coating solution to enhance the biocompatibility of the scaffold. The structure and morphology of the fabricated scaffolds are analyzed, and the mechanical properties are investigated as well. Moreover, the in vitro and in vivo experiments demonstrate the biocompatibility of the materials and the fabrication process. In conclusion, these results demonstrate that the composite scaffold is effective and holds great potential for skin regeneration in the clinic.

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“…(MS2b) Localization of encapsulated cells in the outer layer of the DEM-MA matrix-core/shell capsule (cells nuclei: blue-4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI); F-actin filaments: red-phalloidin). [64,[67][68][69][70][71][72][73][74][75][76] Microfibers are continuously formed in a microchannel with a coaxial flow composed by the fiber precursor solution and sheathed by the crosslinking agent. Adapted with permission.…”

Section: Fiber-shaped Systemsmentioning

confidence: 99%

Design Principles and Multifunctionality in Cell Encapsulation Systems for Tissue Regeneration

Correia

Reis

Mano

2018

Adv Healthcare Materials

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Cell encapsulation systems are being increasingly applied as multifunctional strategies to regenerate tissues. Lessons afforded with encapsulation systems aiming to treat endocrine diseases seem to be highly valuable for the tissue engineering and regenerative medicine (TERM) systems of today, in which tissue regeneration and biomaterial integration are key components. Innumerous multifunctional systems for cell compartmentalization are being proposed to meet the specific needs required in the TERM field. Herein is reviewed the variable geometries proposed to produce cell encapsulation strategies toward tissue regeneration, including spherical and fiber-shaped systems, and other complex shapes and arrangements that better mimic the highly hierarchical organization of native tissues. The application of such principles in the TERM field brings new possibilities for the development of highly complex systems, which holds tremendous promise for tissue regeneration. The complex systems aim to recreate adequate environmental signals found in native tissue (in particular during the regenerative process) to control the cellular outcome, and conferring multifunctional properties, namely the incorporation of bioactive molecules and the ability to create smart and adaptative systems in response to different stimuli. The new multifunctional properties of such systems that are being employed to fulfill the requirements of the TERM field are also discussed.

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Electrospinning versus microfluidic spinning of functional fibers for biomedical applications

Cited by 295 publications

References 254 publications

Simple Fabrication of Multicomponent Heterogeneous Fibers for Cell Co‐Culture via Microfluidic Spinning

Simple Fabrication of Multicomponent Heterogeneous Fibers for Cell Co‐Culture via Microfluidic Spinning

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

Design Principles and Multifunctionality in Cell Encapsulation Systems for Tissue Regeneration

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