Electrospinning of collagen nanofibers: Effects on the behavior of normal human keratinocytes and early-stage wound healing

Rho, Kyong Su; Jeong, Lim; Lee, Gene; Seo, Byoung‐Moo; Park, Yoon Jeong; Hong, Seong‐Doo; Roh, Sangho; Cho, Jae Jin; Park, Won Ho; Min, Byung‐Moo

doi:10.1016/j.biomaterials.2005.08.004

Cited by 792 publications

(521 citation statements)

References 21 publications

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“…The PLLA mesh gradually detached from the wound bed accompanied with infiltration of inflammatory cells and maturation of epidermis. Some researchers also reported the inflammatory responses and separation of implanted materials from regenerated skin, which is a common phenomenon in the wound healing [22][23][24][25][26].…”

Section: Discussionmentioning

confidence: 99%

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

Naoki

et al. 2012

Science and Technology of Advanced Materials

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In skin tissue engineering, a three-dimensional porous scaffold is necessary to support cell adhesion and proliferation and to guide cells moving into the repair area in the wound healing process. Structurally, the porous scaffold should have an open and interconnected porous architecture to facilitate homogenous cell distribution. Moreover, the scaffolds should be mechanically strong to protect deformation during the formation of new skin. In this study, the hybrid scaffolds were prepared by forming funnel-like collagen or gelatin sponge on a woven poly(l-lactic acid) (PLLA) mesh. The hybrid scaffolds combined the advantages of both collagen or gelatin (good cell-interactions) and PLLA mesh (high mechanical strength). The hybrid scaffolds were used to culture dermal fibroblasts for dermal tissue engineering. The funnel-like porous structure promoted homogeneous cell distribution and extracellular matrix production. The PLLA mesh reinforced the scaffold to avoid deformation. Subcutaneous implantation showed that the PLLA-collagen and PLLA-gelatin scaffolds promoted the regeneration of dermal tissue and epidermis and reduced contraction during the formation of new tissue. These results indicate that funnel-like hybrid scaffolds can be used for skin tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

Naoki

et al. 2012

Science and Technology of Advanced Materials

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“…Moreover, cell growth on electrospun scaffolds was better than that on the film scaffolds (Figure 4, PLA vs. PLAF, SHPXL, XL-L, XL-M, and XL-H vs. ZFXL), since the porous structure of electrospun fiber mats was able to facilitate the transportation of oxygen, nutrients, and the metabolic waste of cells, and the migration of cells and communication between them, all of which contribute to better cell growth [15,[39][40][41]. Furthermore, after 24h fewer cells were growing on the electrospun zein fibers cross-linked using SHP (SHPXL) than on samples XL-L, XL-M, and XL-H cross-linked without SHP.…”

Section: Cell Growthmentioning

confidence: 99%

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

2010

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“…However, POM is soluble in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), which is frequently used in electrospinning of natural biopolymers such as collagen and chitin. [10][11][12][13] In this work, electrospinning of POM solutions in HFIP is investigated, and to our surprise, a very high elongation to break was observed with the POM electrospun fiber mats. Figure 1 shows the morphology of the electrospun POM mat obtained from a 5 wt % solution at 30°C with a relative humidity of 40%.…”

mentioning

confidence: 94%

High-Elongation Fiber Mats by Electrospinning of Polyoxymethylene

Zhang

Xiang-zhong

et al. 2008

Macromolecules

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Electrospinning is a recently explored simple and versatile fabrication technique for producing nano-to microscale fibers. The electrospun fiber mats possess a number of characteristics such as high specific surface area, high aspect ratio, and high porosity as a result of random deposition of the fibers, which allow a wide range of potential applications such as optoelectronics, sensor technology, catalysis, filtration, and medicine. 1,2 The mechanical properties of the electrospun fibers or mats are usually different from those of the corresponding bulk materials. Sometimes, the difference is enormous and surprising. [3][4][5] For example, Gu et al. 4 reported a Young's modulus of 50 GPa for polyacrylonitrile single nanofibers, which is much greater than that of the corresponding cast film (1.2 GPa). Kim et al. 5 observed the phenomenon of "necking" with poly(methyl methacrylate) (PMMA)/montmorillonite nanocomposite electrospun single fibers, whereas PMMA is known as a brittle plastic.Polyoxymethylene (POM) is a versatile engineering plastic. 6,7 It is widely used in automobile and electronic industries due to good properties such as good strength, stiffness, abrasion, and chemical resistance. 8,9 Although almost 100 polymer solutions or melts have been electrospun into fibers, 4 to the best of our knowledge, electrospinning of POM has not yet been reported due to presumably poor solubility in common solvents. However, POM is soluble in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), which is frequently used in electrospinning of natural biopolymers such as collagen and chitin. [10][11][12][13] In this work, electrospinning of POM solutions in HFIP is investigated, and to our surprise, a very high elongation to break was observed with the POM electrospun fiber mats. Figure 1 shows the morphology of the electrospun POM mat obtained from a 5 wt % solution at 30°C with a relative humidity of 40%. The average diameter is 940 nm, and microporous structure was observed with an average pore size of 150 nm. It is well-known that microporous structure is frequently obtained when volatile solvents are used for electrospinning, 14,15 and that high relative humidity also favors formation of pores. 1,16 HFIP is a volatile solvent with a boiling point of 58°C. The formation of microporous structure in the current case is understandable, although the electrospun fibers of biopolymers reported so far have no obvious pores. [10][11][12][13] The mechanical properties of the nonwoven POM electrospun mats were measured by tensile tests after thorough drying at 40°C under vacuum, and a typical load-elongation curve is shown in Figure 2a. Elongation of 460% was observed. For comparison, the elongation at break of bulk POM such as a tensile bar obtained from injection molding is known to be 40-50%, 9 and that of the thin film cast from the same solution used for electrospinning is only 1%. Therefore, the elongation at break of the electrospun nonwoven mat is about 10-fold of that of the bulk tensile bar and about 460-fold of that of the...

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Electrospinning of collagen nanofibers: Effects on the behavior of normal human keratinocytes and early-stage wound healing

Cited by 792 publications

References 21 publications

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

High-Elongation Fiber Mats by Electrospinning of Polyoxymethylene

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