Electrospinning of Collagen Nanofibers

Matthews, Janice R.; Wnek, Gary E.; Simpson, David G.; Bowlin, Gary L.

doi:10.1021/bm015533u

Cited by 2,089 publications

(1,385 citation statements)

References 17 publications

Supporting

Mentioning

1,305

Contrasting

Unclassified

Order By: Relevance

“…Exposing the electrospun scaffold to glutaraldehyde intermolecularly cross-linked the scaffolds, making cell culturing possible; however, cross-linking reduced the porosity dramatically. Currently, only glutaraldehyde has been investigated as a cross-linking agent for electrospun collagen based structures [3,16,42,43]. However, glutaraldehyde-treated materials can be cytotoxic [44].…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

View full text Add to dashboard Cite

Electrospinning using natural proteins or synthetic polymers is a promising technique for the fabrication of fibrous scaffolds for various tissue engineering applications. However, one limitation of scaffolds electrospun from natural proteins is the need to cross-link with glutaraldehyde for stability, which has been postulated to lead to many complications in vivo including graft failure. In this study, we determined the characteristics of hybrid scaffolds composed of natural proteins including collagen and elastin, as well as gelatin, and the synthetic polymer poly(ε-caprolactone) (PCL), so to avoid chemical cross-linking. Fiber size increased proportionally with increasing protein and polymer concentrations, whereas pore size decreased. Electrospun gelatin/PCL scaffolds showed a higher tensile strength when compared to collagen/elastin/PCL constructs. To determine the effects of pore size on cell attachment and migration, both hybrid scaffolds were seeded with adipose-derived stem cells. Scanning electron microscopy and nuclei staining of cell-seeded scaffolds demonstrated complete cell attachment to the surfaces of both hybrid scaffolds, although cell migration into the scaffold was predominantly seen in the gelatin/PCL hybrid. The combination of natural proteins and synthetic polymers to create electrospun fibrous structures resulted in scaffolds with favorable mechanical and biological properties.

show abstract

Section: Discussionmentioning

confidence: 99%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Once soluble, the proteins can be dialyzed, lyophilized and resolubilized in organic solvents such as hexafluoroisopropanol or in water for limited periods of time depending on concentration. In the case of fiber formation, silks [65,66] and collagens [67] have been electrospun to form nanometer-scale diameter fibers. Thus, modulation in fiber diameters from the native 10's of microns in the case of B. mori to submicron by electrospinning offers a range of morphologies for cell interactions.…”

Section: Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Silk-based biomaterials

et al. 2003

View full text Add to dashboard Cite

Silk from the silkworm, Bombyx mori, has been used as biomedical suture material for centuries. The unique mechanical properties of these fibers provided important clinical repair options for many applications. During the past 20 years, some biocompatibility problems have been reported for silkworm silk; however, contamination from residual sericin (glue-like proteins) was the likely cause. More recent studies with well-defined silkworm silk fibers and films suggest that the core silk fibroin fibers exhibit comparable biocompatibility in vitro and in vivo with other commonly used biomaterials such as polylactic acid and collagen. Furthermore, the unique mechanical properties of the silk fibers, the diversity of side chain chemistries for 'decoration' with growth and adhesion factors, and the ability to genetically tailor the protein provide additional rationale for the exploration of this family of fibrous proteins for biomaterial applications. For example, in designing scaffolds for tissue engineering these properties are particularly relevant and recent results with bone and ligament formation in vitro support the potential role for this biomaterial in future applications. To date, studies with silks to address biomaterial and matrix scaffold needs have focused on silkworm silk. With the diversity of silk-like fibrous proteins from spiders and insects, a range of native or bioengineered variants can be expected for application to a diverse set of clinical needs. r

show abstract

“…Although this technique is a good approach to mimic the ECM, it has so far failed to control fibre orientation. The distribution and arrangement of the ECM has a crucial role in controlling cell shape, regulating physiological function and defining organ architecture and it is believed that controlling the fibre orientation will increase biomimetics [33].…”

Section: Phase Separationmentioning

confidence: 99%