Electroretinographic study of the magnetic compass in European robins

Abstract

Migratory birds are known to be sensitive to external magnetic field (MF). Much indirect evidence suggests that the avian magnetic compass is localized in the retina. Previously, we showed that changes in the MF direction could modulate retinal responses in pigeons. In the present study, we performed similar experiments using the traditional model animal to study the magnetic compass, European robins. The photoresponses of isolated retina were recorded using ex vivo electroretinography (ERG). Blue- and red-light stimuli were applied under an MF with the natural intensity and two MF directions, when the angle between the plane of the retina and the field lines was 0° and 90°, respectively. The results were separately analysed for four quadrants of the retina. A comparison of the amplitudes of the a- and b-waves of the ERG responses to blue stimuli under the two MF directions revealed a small but significant difference in a- but not b-waves, and in only one (nasal) quadrant of the retina. The amplitudes of both the a- and b-waves of the ERG responses to red stimuli did not show significant effects of the MF direction. Thus, changes in the external MF modulate the European robin retinal responses to blue flashes, but not to red flashes. This result is in a good agreement with behavioural data showing the successful orientation of birds in an MF under blue, but not under red illumination.

The Magnetic Compass of Birds: The Role of Cryptochrome

“…Experimental evidence meanwhile supports the Radical Pair-Model. In an electro-retinographic study, differences in responses to varying magnetic directions were recorded in the retina under blue, but not under red light ( Astakhova et al, 2020 ). Radio-frequency fields in the MHz (MegaHertz)-range are a diagnostic test for the involvement of radical pair processes (see, e.g., Ritz, 2001 ; Henbest et al, 2004 ); such radio-frequency fields indeed cause disorientation in birds ( Figure 1 ; e.g., Ritz et al, 2004 , 2009 ; Thalau et al, 2005 ; Kavokin et al, 2014 ; Bojarinova et al, 2020 ).…”