Electroretinographic signs of retinal remodeling after experimental induction of retinal pigment epithelium atrophy

Нероев, В. В.; Нероева, Н. В.; Mv, Zueva; Катаргина, Л. А.; Цапенко, И. В.; Ilyukhin, P. A.; Лосанова, О. А.; Karmokova, A. G.; Rogov, S.V.

doi:10.17116/oftalma202113704124

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…This indicates the inhibition of the functional activity of the second-order neurons in the peripheral parts of the retina and the activation of Müller cells in response to retinal hypoxia. 20 , 21 Taking into account that Cg in patients with progression of NPDR was initially higher than in patients without progression of NPDR, this indicator, in combination with the results of other instrumental (OCT, Doppler ultrasonography, and FAG) and laboratory studies (HIF1-α and EPO), can serve as a criterion for predicting the development of PDR, which is also the novelty of this study.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Hypoxia and Microcirculation Factors in the Progression of Diabetic Retinopathy

Majidova

2024

Invest. Ophthalmol. Vis. Sci.

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Purpose The purpose of this study was to evaluate comparatively the changes in HIF-1ɑ, EPO, sICAM-1, hemodynamic, and electrophysiological parameters during the progression of non-proliferative diabetic retinopathy (NPDR). Methods This retrospective longitudinal study included 82 patients with NPDR, who were divided into 2 groups: group I ( n = 40) consisted of patients without progression of NPDR after 1 year and group II ( n = 42) included patients with the transition of NPDR to proliferative diabetic retinopathy (PDR). The hemodynamics of the eye was assessed by Doppler ultrasonography. The glial hypoxia index Cg was calculated using ERG. The serum levels of hypoxia-inducible factor 1-a (HIF1-α), soluble intercellular adhesion molecule-1 (sICAM-1), and erythropoietin (EPO) were determined by ELISA method. Results In group II, resistive index (RI), short posterior ciliary arteries (SPCAs) increased significantly from 0.62 ± 0.005 to 0.65 ± 0.007 ( P = 0.003), being higher than the corresponding parameter in group I ( P = 0.013). In group II, there was an increase in the hypoxia index Cg (5.56 ± 0.05) relative to the primary indicators and in group I ( P < 0.001). In group II, HIF1-ɑ, EPO, and sICAM-1 levels after a year significantly increased (0.213 ± 0.02 ng/mL, 37.7 ± 2.4 mIU/mL, and 576.3 ± 11.9 ng/mL, respectively) both relative to the main indicators and the values in group I ( P < 0.001). When EPO exceeds 27.5 mIU/mL, a high risk of progression of NPDR to the initial stages of PDR is predicted. Conclusions The glial Cg index and the level of HIF1-a, EPO in the serum of patients with progression of NPDR were initially higher than in patients without progression of NPDR and have increased during the year, indicating the development of PDR due to more severe hypoxia.

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Section: Discussionmentioning

confidence: 99%

“…The nature of changes in this index can provide information about the functional activity of Müller cells and the presence of retinal hypoxia in the initial stages of circulatory disorders in the retina. 20 , 21 …”

Section: Methodsmentioning

confidence: 99%

Evaluation of Hypoxia and Microcirculation Factors in the Progression of Diabetic Retinopathy

Majidova

2024

Invest. Ophthalmol. Vis. Sci.

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Characteristic vitreous changes in congenital cataracts

et al. 2015

Vestn. oftal'mol.

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Changes of alpha-2-macroglobulin activity in tear fluid in experimental retinal pigment epithelium atrophy of rabbits

Нероева

Чеснокова

Катаргина

et al. 2022

Rossijskij oftalʹmologičeskij žurnal

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Purpose. To assess the validity of alpha-2-macroglobulin ( 2-MG) activity and endothelin-1 (ET-1) concentration for the characterization of local metabolic disorders in experimental retinal pigment epithelium atrophy (RPE).Material and methods. To reproduce RPE atrophy, 22 New Zealand Albino rabbits were given a subretinal injection of bevacizumab or saline. Tear fluid was collected before the injection and 3 months after it. In the second series of the experiment, tear fluid was also collected on the 3rd, 7th, 14th, 21st and 28th days after bevacizumab injection. Tear fluid was analyzed for the activity of 2-MG using the fermentation method and for ET-1 concentration by the immunoenzymatic method.Results. 3 months after bevacizumab injection, 2-MG activity in the tear remained normal, while after saline injection it was, on average, twice as high as the initial one. ET-1 concentration showed a significant increase of over 1.5 times on the 3rd day after bevacizumab injection both in the tear of the operated and the contralateral eyes.Conclusion. Subretinal bevacizumab injection had no significant lasting damaging effect on the retina, as opposed to saline injection that led to an increase of 2-MG activity in the tear. A transitory increase of ET-1 concentration in the tears after bevacizumab injection may indicate vascular tone elevation in the eye during this period. The study of 2-MG activity and ET-1 concentration in the tear may be used to monitor local metabolic shifts in experimental RPE atrophy development, as well as to assess the post-transplantation process and therapy adequacy.

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Electroretinographic signs of retinal remodeling after experimental induction of retinal pigment epithelium atrophy

Cited by 3 publications

References 15 publications

Evaluation of Hypoxia and Microcirculation Factors in the Progression of Diabetic Retinopathy

Evaluation of Hypoxia and Microcirculation Factors in the Progression of Diabetic Retinopathy

Characteristic vitreous changes in congenital cataracts

Changes of alpha-2-macroglobulin activity in tear fluid in experimental retinal pigment epithelium atrophy of rabbits

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