Electropotentials of tumor tissue

Schauble, Muriel K.; Habal, Mutaz B.

doi:10.1016/0022-4804(69)90127-9

Cited by 11 publications

(9 citation statements)

References 4 publications

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“…Thus, physiological electric fields may contribute to the regulation and steering of endothelial migration, supplementing or modulating the effects of the well-known chemical signals that promote angiogenesis. Interestingly, solid tumors have also been shown to generate electric fields with the tumor more negative than the surrounding tissue [28]. The magnitude of these field-associated changes is not precisely known, but the direction is such that endothelial cells would be induced to move toward the lesion as in surface wounds.…”

Section: Locomotive Response Of Endothelial Cells To Electric Fieldsmentioning

confidence: 99%

Effects of Direct Current Electric Fields on Cell Migration and Actin Filament Distribution in Bovine Vascular Endothelial Cells

Kolega²

2002

J Vasc Res

165

119

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Electric fields exceeding 1 V/cm occur during wound healing, morphogenesis, and tumor growth, and such fields have been shown to induce directional migration of a variety of different cells. However, the mechanism by which electric fields direct cell movement is not yet understood, and the effects on vascular endothelial cells are entirely unknown. We demonstrate that cultured bovine aortic endothelial cells migrate toward the cathode of an applied electric field. Time-lapse microscopic imaging shows that the field suppresses protrusive activity from anode-facing surfaces of the cells while stimulating protrusions from surfaces that face the cathode. The threshold for this response is 1–2 V/cm, similar to field strengths measured in vivo. In addition, fluorescence microscopy shows that lamellipodia projecting toward the cathode are rich in actin filaments. Using quantitative image analysis, we show that the electric field induces a transient 80% increase in the amount of filamentous actin in the cell. Comparison of the distribution of F-actin with total protein distribution indicates that F-actin is asymmetrically distributed in the cytoplasm, being selectively enriched toward the cathode. We propose that physiological electric fields direct cell migration by eliciting an intracellular signal that creates new sites for actin assembly in the cathodal cytoplasm.

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Section: Locomotive Response Of Endothelial Cells To Electric Fieldsmentioning

confidence: 99%

Effects of Direct Current Electric Fields on Cell Migration and Actin Filament Distribution in Bovine Vascular Endothelial Cells

Kolega²

2002

J Vasc Res

165

119

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“…Tumor tissues possess a more negative surface electropotential than normal tissue in both experimental animals and humans, and the degree of electronegativity may be directly correlated with the degree of biological malignancy and differentiation of the tumor [5,6,7,8,9].…”

Section: Introductionmentioning

confidence: 99%

Pharmacodynamics induced by direct electric current for the treatment of 5-fluorouracil resistant tumor: an animal experiment

Ito

Wong

Ando

et al. 2001

Int J Colorectal Dis

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The repeated use of chemotherapy to treat patients with colorectal carcinoma may be limited by the fact it creates resistance cells. However, we have observed a remarkable decrease in certain types of drug resistance in patients treated with direct electric current. Experimental studies were therefore performed in animals to determine the differences in pharmacodynamics between chemotherapy with and that without electric treatment. Tumors were created in BALB/c mice by intradermal injection of 0.25 ml 4 x 10(6) Colon 26 cells/ml in the abdomen. Seven days later the mice were divided into two groups: controls and those that underwent electric treatment. Direct electric current (1,000 V, 0.2-0.8 microA) was passed between a platinum electrode inserted intradermally and the earth during and for 1 h after a single intravenous injection of 5-fluorouracil (5-FU; 12.5 mg ml(-1) kg(-1)). Peripheral blood samples were collected before and 5, 10, 20, 30, 45, and 60 min after the injection of 5-FU. Concentrations of 5-FU in the sera and tissues were measured by HPLC. The intratumoral concentrations of 5-FU in the electric treatment group were higher than those in the controls (P<0.05, two-factor analysis of variance), but the serum concentrations were not statistically different between the two groups. Pharmacodynamic changes were thus observed as a result of electrostatic treatment during chemotherapy. This elevated 5-FU concentration in the tumor tissue is considered one of the reasons for the effective inhibition of 5-FU resistance in clinical cases.

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“…We observed that the biopotential distribution seemed to be particularly affected by the growth direction, the growth rate, and the existence of a nodular structure within some tumors. The relatively few puncture wounds (3)(4)(5) per measurement session probably resulted in a considerable spatial undersampling of the biopotential gradients in tumor tissue. In two cases there appeared to be bioelectric gradients of about 1 mV/mm near a rapidly growing tumor edge.…”

Section: Discussionmentioning

confidence: 99%

“…The literature reports that biopotential electrodes placed in tumors show a net electropotential relative to surrounding healthy tissue and that the growth and/or malignancy of the tumor is related to bioelectrical changes (1)(2)(3)(4)(5). Electrical anomalies in skin surface potentials have been detected over tumors by several investigators suggesting that the underlying tumors are electroactive (2,s).…”

Section: Introductionmentioning

confidence: 99%

“…There has been considerable interest in the apparent association of biopotentials with growing cancer tumors (1)(2)(3)(4)(5). The literature reports that biopotential electrodes placed in tumors show a net electropotential relative to surrounding healthy tissue and that the growth and/or malignancy of the tumor is related to bioelectrical changes (1)(2)(3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of Cancer Tumor Biopotential in Relation to Tumor Ph

Towe

Shirazi

Kakavand

1996

Electro- and Magnetobiology

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Biopotentials associated with adenocarcinomas in mice and hamsters were measured using a tumor-penetrating microelectrode system. Tumor ionic compositions and pH were determined by microsampling and by pH microelectrodes. We observed that the pH gradient through the tumor mass approximately follows the tumor biopotential gradient. We show that the magnitude of the observed tumor biopotentials can be explained in terms of Nemst-Planck diffusion potentials arising from tumor pH gradients.

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Electropotentials of tumor tissue

Cited by 11 publications

References 4 publications

Effects of Direct Current Electric Fields on Cell Migration and Actin Filament Distribution in Bovine Vascular Endothelial Cells

Effects of Direct Current Electric Fields on Cell Migration and Actin Filament Distribution in Bovine Vascular Endothelial Cells

Pharmacodynamics induced by direct electric current for the treatment of 5-fluorouracil resistant tumor: an animal experiment

Investigation of Cancer Tumor Biopotential in Relation to Tumor Ph

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