Electroporation of the photosynthetic membrane: structural changes in protein and lipid-protein domains

Rosemberg, Yosef; Rotenberg, Michal; Korenstein, Rafi

doi:10.1016/s0006-3495(94)80571-5

Cited by 9 publications

(2 citation statements)

References 38 publications

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“…Mathematical prediction of the three-state model for the lifetime of electropermeabilization (Teissié and Rols, 1994) The long-lived electropermeabilized state implies that proteins are involved in cell membrane electropermeabilization. This is supported by experiments (Teissié and Tsong, 1980;Rosemberg et al, 1994). The field effect was modeled by a three-state scheme: the intact membrane (state A) is brought to a highly unstable state B by the pulse; B is spontaneously converted to the metastable state C:…”

Section: Appendixmentioning

confidence: 72%

Electropermeabilization of Mammalian Cells to Macromolecules: Control by Pulse Duration

Rols

Teissié

1998

Biophysical Journal

309

242

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Membrane electropermeabilization to small molecules depends on several physical parameters (pulse intensity, number, and duration). In agreement with a previous study quantifying this phenomenon in terms of flow (Rols and Teissié, Biophys. J. 58:1089-1098, 1990), we report here that electric field intensity is the deciding parameter inducing membrane permeabilization and controls the extent of the cell surface where the transfer can take place. An increase in the number of pulses enhances the rate of permeabilization. The pulse duration parameter is shown to be crucial for the penetration of macromolecules into Chinese hamster ovary cells under conditions where cell viability is preserved. Cumulative effects are observed when repeated pulses are applied. At a constant number of pulses/pulse duration product, transfer of molecules is strongly affected by the time between pulses. The resealing process appears to be first-order with a decay time linearly related to the pulse duration. Transfer of macromolecules to the cytoplasm can take place only if they are present during the pulse. No direct transfer is observed with a postpulse addition. The mechanism of transfer of macromolecules into cells by electric field treatment is much more complex than the simple diffusion of small molecules through the electropermeabilized plasma membrane.

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Section: Appendixmentioning

confidence: 72%

Electropermeabilization of Mammalian Cells to Macromolecules: Control by Pulse Duration

Rols

Teissié

1998

Biophysical Journal

309

242

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“…Such changes may be caused by lipid-protein interactions (Woolf and Roux, 1994). These interactions could stabilize or destabilize the membrane, and the nature of the interaction may be changed if the proteins undergo electric field-induced changes (Chen and Lee, 1994;Rosemberg et al, 1994). Membrane proteins could also alter the electric field across the bilayer.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Gramicidin on Electroporation of Lipid Bilayers

Troiano

Stebe

Raphael

et al. 1999

Biophysical Journal

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The effects of the channel-forming peptide gramicidin D (gD) on the conductance and electroporation thresholds of planar bilayer lipid membranes, made of the synthetic lipid 1-palmitoyl 2-oleoyl phosphatidylcholine (POPC), was studied. High-amplitude ( approximately 200-900 mV) rectangular voltage pulses of 15 ms duration were used to perturb the bilayers and monitor the transmembrane conductance. Electroporation voltage thresholds were found, and conductance was recorded before and after electroporation. Gramicidin was added to the system in peptide/lipid ratios of 1:10, 000, 1:500, and 1:15. The addition of gD in a ratio of 1:10,000 had no effect on electroporation, but ratios of 1:500 and 1:15 significantly increased the thresholds by 16% (p < 0.0001) and 40% (p < 0.0001), respectively. Membrane conductance before electroporation was measurable only after the addition of gD and increased monotonically as the peptide/lipid ratio increased. The effect of gD on the membrane area expansivity modulus (K) was tested using giant unilamellar vesicles (GUVs). When gD was incorporated into the vesicles in a 1:15 ratio, K increased by 110%, consistent with the increase in thresholds predicted by an electromechanical model. These findings suggest that the presence of membrane proteins may affect the electroporation of lipid bilayers by changing their mechanical properties.

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Electroluminescence

Gorkom

1996

Photosynth Res

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An overview is presented of research based on the observation by Arnold and Azzi (1971) (Photochem Photobiol 14: 233-240), that an electric field induces charge-recombination luminescence in a suspension of photosynthetic membrane vesicles. The 'electroluminescence' signals from Photosystems I and II are discussed in relation to the shape of the vesicles and the membrane potentials generated by the externally applied electric field. The use of the electroluminescence amplitude as a probe to study the kinetics and energetics of charge separation, and of its kinetics to monitor the electric-field induced charge recombination process are reviewed. Currently unresolved issues regarding the emission yield of electroluminescence are briefly discussed and the properties are summarized of the unexplained Photosystem II luminescence which is not sensitive to the membrane potential.

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Electroporation of the photosynthetic membrane: structural changes in protein and lipid-protein domains

Cited by 9 publications

References 38 publications

Electropermeabilization of Mammalian Cells to Macromolecules: Control by Pulse Duration

Electropermeabilization of Mammalian Cells to Macromolecules: Control by Pulse Duration

The Effects of Gramicidin on Electroporation of Lipid Bilayers

Electroluminescence

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