“…Techniques for incorporating recombinant DNA into a genome is a key feature for a model organism. For Thalassiosira pseudonana , this has so far been achieved by biolistic particle delivery (Poulsen et al, 2006), electroporation (Bugge, 2015), and bacterial conjugation (Karas et al, 2015). These methods rely on positive selection based on resistance to the antibiotics nourseothricin ( nat gene; Poulsen et al, 2006), zeocin ( ble gene; Scheffel et al, 2011), and blasticidin ( bsr gene; Heintze et al, 2022) with expression of the antibiotic resistance gene under control of endogenous promoters.…”