Electroporation of cells and tissues

Weaver, James C.

doi:10.1109/27.842820

Cited by 350 publications

(156 citation statements)

References 99 publications

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“…11 In addition, in some cases the effects of the field can damage biological materials and alter their properties. [12][13][14][15][16][17][18] These undesirable outcomes can be eliminated or greatly reduced if the sensing is performed at higher gigahertz frequencies, where capacitance sensitivity tends to increase. 19 Detection systems based on flow impedance measurements 20,21 have several advantages over the more traditional optical methods.…”

Section: Motivationmentioning

confidence: 99%

Microwave frequency sensor for detection of biological cells in microfluidic channels

Nikolic-Jaric¹,

Romanuik²,

Ferrier³

et al. 2009

Biomicrofluidics

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We present details of an apparatus for capacitive detection of biomaterials in microfluidic channels operating at microwave frequencies where dielectric effects due to interfacial polarization are minimal. A circuit model is presented, which can be used to adapt this detection system for use in other microfluidic applications and to identify ones where it would not be suitable. The detection system is based on a microwave coupled transmission line resonator integrated into an interferometer. At 1.5 GHz the system is capable of detecting changes in capacitance of 650 zF with a 50 Hz bandwidth. This system is well suited to the detection of biomaterials in a variety of suspending fluids, including phosphate-buffered saline. Applications involving both model particles ͑polystyrene microspheres͒ and living cells-baker's yeast ͑Saccharomyces cerevisiae͒ and Chinese hamster ovary cells-are presented.

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Section: Motivationmentioning

confidence: 99%

Microwave frequency sensor for detection of biological cells in microfluidic channels

Nikolic-Jaric¹,

Romanuik²,

Ferrier³

et al. 2009

Biomicrofluidics

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“…The external application of an electric Weld (·1 KV/cm) induces a potential diVerence of »0.3-1 V across the cytoplasmic membrane for a period long enough (microseconds to milliseconds) to induce pore formation in the plasma membrane (Weaver 2000). Depending on the cell properties (i.e.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Galindo

Dejmek

Lundgren

et al. 2009

Planta

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Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

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“…When the membrane potential reaches a critical value which is around 0.2 − 1.5 V 4 dielectric membrane breakdown occurs resulting in the formation of transient pores [5][6][7] . Electroporation is most frequently used to introduce charged, polar molecules such as DNA, dyes, drugs or proteins into the interior of the cell.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Maximization of Cell Permeabilization and Viability in Single-Cell Electroporation Using an Electrolyte-Filled Capillary

et al. 2006

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A549 cells were briefly exposed to Thioglo −1 which converts thiols to fluorescent adducts. The fluorescent cells were exposed to short (50 − 300 ms) electric field pulses (500 V across a 15 cm capillary) created at the tip of an electrolyte -filled capillary. Fluorescence microscopy revealed varying degrees of cell permeabilization depending on conditions. Longer pulses and shorter cellcapillary tip distance led to a greater decrease in a cell's fluorescence. Live/dead (calcein AM and propidium iodide) testing revealed that a certain fraction of cells died. Longer pulses and shorter cell -capillary tip distances were more deadly. An optimum condition exists at a cell -capillary tip distance of 3.5 μm − 4.5 μm and a pulse duration of 120 ms -150 ms. At these conditions > 90 % of the cells are permeabilized and 80 − 90% survive.

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Electroporation of cells and tissues

Cited by 350 publications

References 99 publications

Microwave frequency sensor for detection of biological cells in microfluidic channels

Microwave frequency sensor for detection of biological cells in microfluidic channels

Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Simultaneous Maximization of Cell Permeabilization and Viability in Single-Cell Electroporation Using an Electrolyte-Filled Capillary

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