Electroporation in rotating electric fields

Gimsa, Jan; Marszałek, Piotr E.; Loewe, Ulrike; Tsong, Tian Yow

doi:10.1016/0302-4598(92)80055-l

Cited by 11 publications

(7 citation statements)

References 6 publications

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“…The electric field strength (E) is given by a Fourier series for a square-wave AC-pulse with a key ratio of 1:1 [36]: d, V ss , j, ω and t stand for the distance of a pair of plane-parallel electrodes, the voltage difference between the electrodes, ffiffiffiffiffi ffi −1 p , the circular frequency, and time, respectively. The Fourier series contains only odd harmonics.…”

Section: Electric Field In the Chambermentioning

confidence: 99%

“…Cells were suspended in 5 ml measuring solution containing 10 µM PI [36]. 7 µl of this suspension with a cell concentration of 0.03% v/v were transferred to the measuring chamber that was sealed by a cover slip.…”

Section: Ep Experimentsmentioning

confidence: 99%

“…Capacitive membrane bridging causes the Δϕ to decrease with increasing field frequency [4,22,[27][28][29][30][31][32][33][34]. Clearly, rotating AC-fields generated by the superposition of two perpendicularly oriented fields are even more effective than the linear AC-fields [28,35,36].…”

Section: Introductionmentioning

confidence: 99%

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Effects of cell orientation and electric field frequency on the transmembrane potential induced in ellipsoidal cells

Maswiwat

Wachner

Gimsa

2008

Bioelectrochemistry

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Section: Electric Field In the Chambermentioning

confidence: 99%

Section: Ep Experimentsmentioning

confidence: 99%

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Effects of cell orientation and electric field frequency on the transmembrane potential induced in ellipsoidal cells

Maswiwat

Wachner

Gimsa

2008

Bioelectrochemistry

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“…Electroporation, in which cells and DNA are subjected to brief high voltage pulses, is thought to involve the transient appearance of pores (3,4), through which the DNA is driven into the cell by the electric field (5,6). Many techniques for producing the electric field have been applied (7)(8)(9)(10)(11). The most common method is capacitor discharge, which produces a field that decays exponentially over time (12).…”

Section: Introductionmentioning

confidence: 99%

Transformation ofEscherichia coliwith large DNA molecules by electroporation

1995

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We have examined bacterial electroporation with a specific interest in the transformation of large DNA, i.e. molecules > 100 kb. We have used DNA from bacterial artificial chromosomes (BACs) ranging from 7 to 240 kb, as well as BAC ligation mixes containing a range o different sized molecules. The efficiency of electroporation with large DNA is strongly dependent on the strain of Escherichia coli used; strains which offer comparable efficiencies for 7 kb molecules differ in their uptake of 240 kb DNA by as much as 30-fold. Even with a host strain that transforms relatively well with large DNA, transformation efficiency drops dramatically with increasing size of the DNA. Molecules of 240 kb transform approximately 30-fold less well, on a molar basis, than molecules of 80 kb. Maximum transformation of large DNA occurs with different voltage gradients and with different time constants than are optimal for smaller DNA. This provides the opportunity to increase the yield of transformants which have taken up large DNA relative to the number incorporating smaller molecules. We have demonstrated that conditions may be selected which increase the average size of BAC clones generated by electroporation and compare the overall efficiency of each of the conditions tested.

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“…High electrode voltages, in combination with small interelectrode gaps, result in high electric field strengths, which may damage the integrity of biological membranes and induce membrane pores by dielectric breakdown at frequencies below the capacitive membrane bridging . Field parameters that permit pore resealing are used for the electrotransfection or electrofusion of vesicles or cells .…”

Section: Introductionmentioning

confidence: 99%

Experimental verification of an equivalent circuit for the characterization of electrothermal micropumps: High pumping velocities induced by the external inductance at driving voltages below 5 V

2013

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Electrothermal micropumps (ETμPs) use local heating to create conductivity and permittivity gradients in the pump medium. In the presence of such gradients, an external AC electric field influences smeared spatial charges in the bulk of the medium. When there is also a symmetry break, the field-charge interaction results in an effective volumetric force resulting in medium pumping. The advantages of the ETμP principle are the absence of moving parts, the opportunity to passivate all the pump structures, homogeneous pump-channel cross-sections, as well as force plateaus in broad frequency ranges. The ETμPs consisted of a DC-heating element and AC field electrodes arranged in a 1000 μm × 250 μm × 60 μm (length × width × height) channel. They were processed as platinum structures on glass carriers. An equivalent-circuit diagram allowed us to model the frequency-dependent pumping velocities of passivated and nonpassivated ETμPs, which were measured at medium conductivities up to 1.0 S/m in the 300 kHz to 52 MHz frequency range. The temperature distributions within the pumps were controlled by thermochromic beads. Under resonance conditions, an additional inductance induced a tenfold pump-velocity increase to more than 50 μm/s at driving voltages of 5 V(rms). A further miniaturization of the pumps is viewed as quite feasible.

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Electroporation in rotating electric fields

Cited by 11 publications

References 6 publications

Effects of cell orientation and electric field frequency on the transmembrane potential induced in ellipsoidal cells

Effects of cell orientation and electric field frequency on the transmembrane potential induced in ellipsoidal cells

Transformation ofEscherichia coliwith large DNA molecules by electroporation

Experimental verification of an equivalent circuit for the characterization of electrothermal micropumps: High pumping velocities induced by the external inductance at driving voltages below 5 V

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