Electrophysiology of Salivary and Pancreatic Acinar Cells

Maruyama, Yoshio

doi:10.1002/cphy.cp060302

Cited by 14 publications

(13 citation statements)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The autonomic sympathetic and parasympathetic nerves release the neurotransmitters noradrenaline (NA) and ACh, respectively. Both ACh and NA trigger mainly a sequence of cellular signal transduction events resulting in intracellular cascades to generate second messengers such as calcium (Ca 2+ ) and adenosine 3,5 cyclic monophosphate (cyclic AMP) which in turn activate ion transport pathways, water, and protein secretion a process known as stimulus secretion coupling (Baum, 1987; Petersen and Gallacher, 1998). Therefore, it seemed logical to investigate the effects of LFN on nerve‐mediated stimulation of amylase secretion in the rat parotid glands.…”

Section: Discussionmentioning

confidence: 99%

Low‐frequency noise effects on the parotid gland of the Wistar rat

et al. 2007

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Low‐frequency noise effects on the parotid gland of the Wistar rat

et al. 2007

View full text Add to dashboard Cite

show abstract

“…IP 3 gates the release of Ca 2+ from intracellular Ca 2+ stores, resulting in Ca 2+ entry from the external medium and a sustained elevation of the intracellular Ca 2+ concentration ([Ca 2+ ] i ) (Hughes et al ., 1988; Baum & Ambudkar, 1988; Mertz et al ., 1990). The resulting increase in [Ca 2+ ] i regulates, directly or indirectly, a number of Ca 2+ ‐dependent ion channels, e.g., the Ca 2+ ‐activated potassium channel in the basolateral membrane and the Ca 2+ ‐activated chloride channel in the luminal membrane, which are intricately involved in salivary secretion in the salivary glands (Putney, 1986; Ambudkar et al ., 1988; Petersen & Gallacher, 1988; Ambudkar, 2000). Thus, phosphoinositide turnover in the salivary glands plays an important role in the regulation of salivary secretion.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of diazepam on muscarinic receptor‐stimulated inositol 1,4,5‐trisphosphate production in rat parotid acinar cells

Kujirai

Sawaki

Kawaguchi

2002

British J Pharmacology

View full text Add to dashboard Cite

1 This study examined the e ect of diazepam (DZP) on phosphoinositide turnover, which plays an important role in the regulation of salivary secretion, in rat parotid acinar cells. 2 DZP (10 79 M to 10 75 M), a potent agonist of both central-and peripheral-type benzodiazepine receptors, dose-dependently decreased inositol 1,4,5-trisphosphate (IP 3 ) production stimulated by carbachol, a muscarinic receptor agonist, in the cells. 3 DZP produced a maximum inhibitory response at a concentration of 10 75 M, with IP 3 production decreased to 63% of maximal levels. The concentration inducing half maximal inhibition of IP 3 production was approximately 3.5610 78 M. 4 An inhibitory response to DZP was produced by a short-term pretreatment (53 min) of the cells and prevented by antagonist and competing ligand for the central-and peripheral-type benzodiazepine receptors,¯umazenil and PK 11195, respectively. 5 DZP showed a non-competitive inhibition of carbachol-stimulated IP 3 production. It did not directly inhibit the activities of GTP-binding regulatory proteins and phosphatidylinositol 4,5-bisphosphate-speci®c phospholipase C (PLC) in the parotid gland membranes, though choline chloride inhibited PLC activity. 6 DZP (10 75 M) attenuated the increase in the intracellular Ca 2+ concentration ([Ca 2+ ] i ) in the cells following stimulation of the muscarinic and a 1 -adrenoceptors. 7 These results suggest that in the parotid acinar cells, DZP inhibits muscarinic receptor-stimulated IP 3 production through benzodiazepine receptors and that PLC activity which produces IP 3 is inhibited by chloride. The decreases in IP 3 and [Ca 2+ ] i in the cells may be connected with the suppression of salivary secretion induced by DZP.

show abstract

“…Calcium is an important second messenger for fluid secretion and exocytosis of secretory cells (Petersen 1992;Petersen and Gallacher 1988). Merritt and Rubin (1985) and Case et al (1988) found that increasing intracellular calcium concentrations and secretory processes are closely related in the pancreas and in large salivary glands.…”

Section: Introductionmentioning

confidence: 99%

Distribution of S-100 protein and its subunits in bovine exocrine glands

Lauboeck

Egerbacher²

1997

Histochemistry and Cell Biology

View full text Add to dashboard Cite

The distribution of S-100 protein and its alpha- and beta-subunits in bovine exocrine glands was studied by indirect immunohistochemistry. The entire spectrum of salivary glands, glands of the respiratory tract, intestinal glands, male and female genital glands, and skin glands was examined. S-100 and its beta-subunit were identified in most serous secretory cells of mixed salivary glands, although secretory acini in some serous glands remained unreactive for these antigens. Mucous cells were constantly negative; mucoid cells were positive in the lacrimal and Harderian gland. The alpha-subunit of S-100 protein was identified in serous cells but the staining reaction was faint. Subunits of S-100 showed a characteristic distribution along the excretory duct systems of compound glands: S-100 and the beta-subunit were present in intercalated duct epithelium, while striated duct epithelium stained for S100-alpha. Therefore, it is suggested that S100-alpha is related to resorption and secretion in striated ducts, while S100-beta may govern acinar exocytosis and probably regulates proliferation and differentiation of glandular cells. Differing staining intensities for S-100 and its subunits in secretory cells of exocrine glands most probably indicate functional differences with regard to secretory activity and the cell cycle.

show abstract

Electrophysiology of Salivary and Pancreatic Acinar Cells

Cited by 14 publications

References 110 publications

Low‐frequency noise effects on the parotid gland of the Wistar rat

Low‐frequency noise effects on the parotid gland of the Wistar rat

Inhibitory effect of diazepam on muscarinic receptor‐stimulated inositol 1,4,5‐trisphosphate production in rat parotid acinar cells

Distribution of S-100 protein and its subunits in bovine exocrine glands

Contact Info

Product

Resources

About