Electrophysiology of dentate gyrus granule cells

Fricke, Russell A.; Prince, D. A.

doi:10.1152/jn.1984.51.2.195

Cited by 157 publications

(74 citation statements)

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“…5B). This was similar to granule cells (Brown et al, 1981;Fricke and Prince, 1984;Lambert and Jones, 1990;Spruston and Johnston, 1992). If larger currents (> −0.5 nA) were tested in pyramidal-shaped cells, rectification was observed in 12 of 17 cells (Fig.…”

Section: V-i Relationssupporting

confidence: 77%

“…This contrasted with many other fast-spiking cells, which do not appear to adapt during such periods of depolarization. Granule cells always adapted during such periods of current injection, and adapted strongly, as has been shown (Fricke and Prince, 1984;Han, 1994). Adaptation occurred in almost all (15 of 17) pyramidal-shaped cells if the duration of the current pulse was increased to 500 ms. Figure 6A shows the response of a strongly adapting cell to a 500-ms pulse, and Figure 6B shows an example of a weakly adapting cell.…”

Section: Discharge Patternssupporting

confidence: 54%

“…Since the membrane properties of granule cells that have been published vary (Brown et al, 1981;Turner and Schwartzkroin, 1983;Fricke and Prince, 1984;Lambert and Jones, 1990;Spruston and Johnston, 1992;Staley et al, 1992), 15 granule cells from the same slices in which the interneurons were impaled were examined for comparative purposes. The mean granule cell RMP was −77.5 ± 1.2 mV (Table 1).…”

Section: Membrane Propertiesmentioning

confidence: 99%

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Electrophysiological diversity of pyramidal‐shaped neurons at the granule cell layer/hilus border of the rat dentate gyrus recorded in vitro

Scharfman

1995

Hippocampus

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In the rat dentate gyrus, pyramidal-shaped cells located on the border of the granule cell layer and the hilus are one of the most common types of γ-aminobutyric acid (GABA)-immunoreactive neurons. This study describes their electrophysiological characteristics. Membrane properties, patterns of discharge, and synaptic responses were recorded intracellularly from these cells in hippocampal slices. Each cell was identified as pyramidal-shaped by injecting the marker Neurobiotin intracellularly (n = 17).In several respects the membrane properties of the sampled cells were similar to "fast-spiking" cells (putative inhibitory interneurons) that have been described in other areas of the hippocampus. For example, input resistance was high (mean 91.3 megohms), the membrane time constant was short (mean 7.7 ms), and there was a large afterhyperpolarization following a single action potential (mean 10.5 mV at resting potential). However, the action potentials of most pyramidalshaped cells were not as brief (mean 1.2 ms total duration) as those of most previously described fast-spiking cells. Many pyramidal-shaped neurons had strong spike frequency adaptation relative to other fast-spiking cells. Although these latter two characteristics were apparent in the majority of the sampled cells, there were exceptional pyramidal-shaped neurons with fast action potentials and weak adaptation, demonstrating the electrophysiological variability of pyramidal-shaped cells.Responses to outer molecular layer stimulation were composed primarily of excitatory postsynaptic potentials (EPSPs) rather than inhibitory postsynaptic potentials (IPSPs), and were usually small (EPSPs evoked at threshold were often less than 2 mV), and brief (less than 30 ms). There was variability, because in a few cells EPSPs evoked at threshold were much larger. However, regardless of EPSP amplitude, suprathreshold stimulation (up to 4 times the threshold stimulus strength) rarely evoked more than one action potential in any cell. The results suggest that stimulation of perforant path axons produces limited excitatory synaptic responses in pyramidal-shaped neurons. This may be one of the reasons why they are relatively resistant to prolonged perforant path stimulation.The pyramidal-shaped neurons located at the base of the granule cell layer have been associated historically with a basket plexus around granule cell somata, and have been called pyramidal "basket" cells. However, basket-like endings were rare and axon collaterals outside the granule cell layer were common. Many axon collaterals were as far from the granule cell layer as the outer molecular layer and the central hilus, and antidromic action potentials could be recorded in some

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Section: V-i Relationssupporting

confidence: 77%

Section: Discharge Patternssupporting

confidence: 54%

Section: Membrane Propertiesmentioning

confidence: 99%

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Electrophysiological diversity of pyramidal‐shaped neurons at the granule cell layer/hilus border of the rat dentate gyrus recorded in vitro

Scharfman

1995

Hippocampus

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“…The signals recorded in control solution consisted of inhibitory and excitatory components (Fricke and Prince 1984). Bicuculline (10 pM) reduced the current, indicating the contribution of a GABA receptor-mediated component.…”

Section: Stimulation Of Inhibitory and Excitatory Inputsmentioning

confidence: 95%

A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system

et al. 1989

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Abstract.(1) A preparation is described which allows patch clamp recordings to be made on mammalian central nervous system (CNS) neurones in situ. (2) A vibrating tissue slicer was used to cut thin slices in which individual neurones could be identified visually. Localized cleaning of cell somata with physiological saline freed the cell membrane, allowing the formation of a high resistance seal between the membrane and the patch pipette. (3) The various configurations of the patch clamp technique were used to demonstrate recording of membrane potential, whole cell currents and single channel currents from neurones and isolated patches. (4) The patch clamp technique was used to record from neurones filled with fluorescent dyes. Staining was achieved by filling cells during recording or by previous retrograde labelling. (5) Thin slice cleaning and patch clamp techniques were shown to be applicable to the spinal cord and almost any brain region and to various species. These techniques are also applicable to animals of a wide variety of postnatal ages, from newborn to adult.

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“…In order to obtain a better understanding of such mechanisms we determined the propagation velocity of epileptiform bursts in hippocampal in-vitro slices, using the 4-aminopyridine epilepsy model in which this activity is confined to the CAI-3 areas (Fricke and Prince 1984;Voskuyl and Albus 1985). Multielectrode recordings were made in the pyramidal layer of the CA1 area, and propagation velocities of spontaneous epileptiform bursts were compared with the values of evoked bursts and the velocities of the fibre systems in CA1.…”

Section: Introductionmentioning

confidence: 99%

Propagation velocity of epileptiform activity in the hippocampus

Holsheimer

Silva

1989

Exp Brain Res

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Summary. The propagation of epileptiform burst activity was investigated in the CA1 area of the in-vitro hippocampal slice preparation of the guinea pig. This activity was provoked by 0.1 mM 4-aminopyridine in the bathing medium and was recorded in the pyramidal layer with an array of eight electrodes. The delay between the first population spike of a burst recorded with different electrodes was calculated using the cross-correlation function. The propagation velocity was estimated from the delays and the electrode intervals. It was found that the velocity of spontaneous and evoked epileptiform bursts varies between 0.15 and 5 m/s and is not confined to the range of conduction velocities of the fibre systems in CA 1 (0.3-0.55 and 1.0-1.8 m/s). Different velocities can be present in different parts of the CA1 area and the initiation of spontaneous bursts is not confined to the CA2-3 areas, but can also occur in CA1. Burst activity also propagated in a low calcium-high magnesium medium. Different mechanisms of propagation are discussed and it is argued that the propagation velocity due to ephaptic interaction may vary largely. It is concluded that epileptiform activity can be propagated not only by synaptic connections at or near the pyramidal layer, but also by way of electrical field effects of population spikes.

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Electrophysiology of dentate gyrus granule cells

Cited by 157 publications

References 0 publications

Electrophysiological diversity of pyramidal‐shaped neurons at the granule cell layer/hilus border of the rat dentate gyrus recorded in vitro

Electrophysiological diversity of pyramidal‐shaped neurons at the granule cell layer/hilus border of the rat dentate gyrus recorded in vitro

A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system

Propagation velocity of epileptiform activity in the hippocampus

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